Characterization of micro-RNA in women with different ovarian reserve

Abstract Women undergoing infertility treatment are routinely subjected to one or more tests of ovarian reserve. Therefore, an adequate assessment of the ovarian reserve is necessary for the treatment. In this study, we aimed to characterize the potential role of microRNAs (miRNAs) as biomarkers for...

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Autores principales:	Masood Abu-Halima, Lea Simone Becker, Basim M. Ayesh, Simona Lucia Baus, Amer Hamza, Ulrike Fischer, Mohamad Hammadeh, Andreas Keller, Eckart Meese
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d75c7636124c4ae4b7dc7fae2934437b
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spelling	oai:doaj.org-article:d75c7636124c4ae4b7dc7fae2934437b2021-12-02T17:14:24ZCharacterization of micro-RNA in women with different ovarian reserve10.1038/s41598-021-92901-w2045-2322https://doaj.org/article/d75c7636124c4ae4b7dc7fae2934437b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92901-whttps://doaj.org/toc/2045-2322Abstract Women undergoing infertility treatment are routinely subjected to one or more tests of ovarian reserve. Therefore, an adequate assessment of the ovarian reserve is necessary for the treatment. In this study, we aimed to characterize the potential role of microRNAs (miRNAs) as biomarkers for women with different ovarian reserves. A total of 159 women were recruited in the study and classified according to their anti-Müllerian hormone (AMH) level into three groups: (1) low ovarian reserve (LAMH, n = 39), (2) normal ovarian reserve (NAMH, n = 80), and (3) high ovarian reserve (HAMH, n = 40). SurePrint Human miRNA array screening and reverse transcription-quantitative PCR (RT-qPCR) were respectively employed to screen and validate the miRNA abundance level in the three tested groups. Compared with NAMH, the abundance level of 34 and 98 miRNAs was found to be significantly altered in LAMH and HAMH, respectively. The abundance level of miRNAs was further validated by RT-qPCR in both, the screening samples as well as in an independent set of validation samples. The abundance levels of the validated miRNAs were significantly correlated with the AMH level. The best AUC value for the prediction of the increase and decrease in the AMH level was obtained for the miR-100-5p and miR-21-5p, respectively. The level of miRNAs abundance correlates with the level of AMH, which may serve as a tool for identifying women with a different ovarian reserve and may help to lay the ground for the development of novel diagnostic approaches.Masood Abu-HalimaLea Simone BeckerBasim M. AyeshSimona Lucia BausAmer HamzaUlrike FischerMohamad HammadehAndreas KellerEckart MeeseNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masood Abu-Halima Lea Simone Becker Basim M. Ayesh Simona Lucia Baus Amer Hamza Ulrike Fischer Mohamad Hammadeh Andreas Keller Eckart Meese Characterization of micro-RNA in women with different ovarian reserve
description	Abstract Women undergoing infertility treatment are routinely subjected to one or more tests of ovarian reserve. Therefore, an adequate assessment of the ovarian reserve is necessary for the treatment. In this study, we aimed to characterize the potential role of microRNAs (miRNAs) as biomarkers for women with different ovarian reserves. A total of 159 women were recruited in the study and classified according to their anti-Müllerian hormone (AMH) level into three groups: (1) low ovarian reserve (LAMH, n = 39), (2) normal ovarian reserve (NAMH, n = 80), and (3) high ovarian reserve (HAMH, n = 40). SurePrint Human miRNA array screening and reverse transcription-quantitative PCR (RT-qPCR) were respectively employed to screen and validate the miRNA abundance level in the three tested groups. Compared with NAMH, the abundance level of 34 and 98 miRNAs was found to be significantly altered in LAMH and HAMH, respectively. The abundance level of miRNAs was further validated by RT-qPCR in both, the screening samples as well as in an independent set of validation samples. The abundance levels of the validated miRNAs were significantly correlated with the AMH level. The best AUC value for the prediction of the increase and decrease in the AMH level was obtained for the miR-100-5p and miR-21-5p, respectively. The level of miRNAs abundance correlates with the level of AMH, which may serve as a tool for identifying women with a different ovarian reserve and may help to lay the ground for the development of novel diagnostic approaches.
format	article
author	Masood Abu-Halima Lea Simone Becker Basim M. Ayesh Simona Lucia Baus Amer Hamza Ulrike Fischer Mohamad Hammadeh Andreas Keller Eckart Meese
author_facet	Masood Abu-Halima Lea Simone Becker Basim M. Ayesh Simona Lucia Baus Amer Hamza Ulrike Fischer Mohamad Hammadeh Andreas Keller Eckart Meese
author_sort	Masood Abu-Halima
title	Characterization of micro-RNA in women with different ovarian reserve
title_short	Characterization of micro-RNA in women with different ovarian reserve
title_full	Characterization of micro-RNA in women with different ovarian reserve
title_fullStr	Characterization of micro-RNA in women with different ovarian reserve
title_full_unstemmed	Characterization of micro-RNA in women with different ovarian reserve
title_sort	characterization of micro-rna in women with different ovarian reserve
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d75c7636124c4ae4b7dc7fae2934437b
work_keys_str_mv	AT masoodabuhalima characterizationofmicrornainwomenwithdifferentovarianreserve AT leasimonebecker characterizationofmicrornainwomenwithdifferentovarianreserve AT basimmayesh characterizationofmicrornainwomenwithdifferentovarianreserve AT simonaluciabaus characterizationofmicrornainwomenwithdifferentovarianreserve AT amerhamza characterizationofmicrornainwomenwithdifferentovarianreserve AT ulrikefischer characterizationofmicrornainwomenwithdifferentovarianreserve AT mohamadhammadeh characterizationofmicrornainwomenwithdifferentovarianreserve AT andreaskeller characterizationofmicrornainwomenwithdifferentovarianreserve AT eckartmeese characterizationofmicrornainwomenwithdifferentovarianreserve
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Characterization of micro-RNA in women with different ovarian reserve

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