Comparison of Molecular Method and Culture in Detection of Community Acquired Pneumonia in Under Five Year Children in Assam, India
Introduction: Pneumonia is the single largest infectious cause of death in children worldwide. Aetiology of pneumonia can be identified using multiple diagnostic tools including culture, serology and Polymerase Chain Reaction (PCR); common pathogens include Streptococcus pneumoniae, Haemophilus...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
JCDR Research and Publications Private Limited
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/d7640b2cc2d848149434eb980c12b791 |
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| Sumario: | Introduction: Pneumonia is the single largest infectious cause
of death in children worldwide. Aetiology of pneumonia can
be identified using multiple diagnostic tools including culture,
serology and Polymerase Chain Reaction (PCR); common
pathogens include Streptococcus pneumoniae, Haemophilus
influenzae, Staphylococcus aureus, some atypical bacteria like
Mycoplasma pneumoniae and Chlamydophila pneumoniae.
Aim: To find out the bacteriological agents causing Community
Acquired Pneumonia (CAP) in under five year children and to
compare the conventional culture and PCR in identifying the
pathogen.
Materials and Methods: This cross-sectional study was undertaken
in the Department of Microbiology and Department of Paediatrics
in a tertiary care centre of Assam, India, between March 2016 to
September 2018. The study was undertaken with 200 under five year
old children who were clinically diagnosed as CAP. Oropharyngeal
(OP) swabs and blood culture were processed for bacteriological
culture. PCR assay of OP swabs for Streptococcus pneumoniae,
Haemophilus influenzae, Staphylococcus aureus etc., including
atypical bacteria like Mycoplasma pneumoniae and Chlamydophila
pneumoniae were performed. Data entry, database management
and statistical analysis were performed using Epi-Info software
version 7.0.
Results: A total of 200 subjects were included in the present study
with a male:female ratio of 1.63:1. Most of the study subjects were
<11 months of age. Most common isolates were Streptococcus
pneumoniae (26.5%) and Staphylococcus aureus (25%) from OP
swabs; blood culture revealed mostly Staphylococcus aureus
(59%) and Streptococcus pneumoniae (25%). PCR assay of OP
swabs were found positive mostly for Staphylococcus aureus
(47%), Streptococcus pneumoniae (21.5%) while two children
were positive for Haemophilus influenzae; Bordetella pertussis
was detected in one child. On evaluation, PCR assay in detecting
the bacterial pathogen was found statistically more significant
than conventional culture of OP swabs (p<0.05).
Conclusion:Staphylococcus aureus and Streptococcus pneumoniae
were the most common bacterial organisms in the study. PCR assay
was found to be more useful in diagnosing the pathogen for bacterial
pneumonia including those difficult to grow in conventional culture. |
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