Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis

Abstract Background Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xuejie Qi, Xiao Geng, Juan Zhang, Binpeng Qu, Xin Zhang, Qiang Jia, Wenhui Yin, Cunxiang Bo, Yan Liu, Hao Li, Linlin Sai, Mingming Han, Cheng Peng
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	RNA methylation M6A LncRNA Amphibious Atrazine Ecology QH540-549.5 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/d76949bbe2674dc5bd8aadcb181c251b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d76949bbe2674dc5bd8aadcb181c251b
record_format	dspace
spelling	oai:doaj.org-article:d76949bbe2674dc5bd8aadcb181c251b2021-11-08T10:45:25ZComprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis10.1186/s41021-021-00223-01880-7062https://doaj.org/article/d76949bbe2674dc5bd8aadcb181c251b2021-11-01T00:00:00Zhttps://doi.org/10.1186/s41021-021-00223-0https://doaj.org/toc/1880-7062Abstract Background Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6A is a highly conserved modification across the species. Results The results of m6A sequences showed that m6A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs. Conclusions These findings suggested that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6A modifications in amphibian species.Xuejie QiXiao GengJuan ZhangBinpeng QuXin ZhangQiang JiaWenhui YinCunxiang BoYan LiuHao LiLinlin SaiMingming HanCheng PengBMCarticleRNA methylationM6ALncRNAAmphibiousAtrazineEcologyQH540-549.5GeneticsQH426-470ENGenes and Environment, Vol 43, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	RNA methylation M6A LncRNA Amphibious Atrazine Ecology QH540-549.5 Genetics QH426-470
spellingShingle	RNA methylation M6A LncRNA Amphibious Atrazine Ecology QH540-549.5 Genetics QH426-470 Xuejie Qi Xiao Geng Juan Zhang Binpeng Qu Xin Zhang Qiang Jia Wenhui Yin Cunxiang Bo Yan Liu Hao Li Linlin Sai Mingming Han Cheng Peng Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
description	Abstract Background Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6A is a highly conserved modification across the species. Results The results of m6A sequences showed that m6A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs. Conclusions These findings suggested that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6A modifications in amphibian species.
format	article
author	Xuejie Qi Xiao Geng Juan Zhang Binpeng Qu Xin Zhang Qiang Jia Wenhui Yin Cunxiang Bo Yan Liu Hao Li Linlin Sai Mingming Han Cheng Peng
author_facet	Xuejie Qi Xiao Geng Juan Zhang Binpeng Qu Xin Zhang Qiang Jia Wenhui Yin Cunxiang Bo Yan Liu Hao Li Linlin Sai Mingming Han Cheng Peng
author_sort	Xuejie Qi
title	Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
title_short	Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
title_full	Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
title_fullStr	Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
title_full_unstemmed	Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis
title_sort	comprehensive analysis of differences of n6-methyladenosine of lncrnas between atrazine-induced and normal xenopus laevis testis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d76949bbe2674dc5bd8aadcb181c251b
work_keys_str_mv	AT xuejieqi comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT xiaogeng comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT juanzhang comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT binpengqu comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT xinzhang comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT qiangjia comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT wenhuiyin comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT cunxiangbo comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT yanliu comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT haoli comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT linlinsai comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT mingminghan comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis AT chengpeng comprehensiveanalysisofdifferencesofn6methyladenosineoflncrnasbetweenatrazineinducedandnormalxenopuslaevistestis
_version_	1718442664887582720

Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis

Ejemplares similares