Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.

There is increasing evidence that breast and other cancers originate from and are maintained by a small fraction of stem/progenitor cells with self-renewal properties. Recent molecular profiling has identified six major subtypes of breast cancer: basal-like, ErbB2-overexpressing, normal breast epith...

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Autores principales:	Xiangshan Zhao, Gautam K Malhotra, Hamid Band, Vimla Band
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d76a11f8453a487d9475118317ee5719
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spelling	oai:doaj.org-article:d76a11f8453a487d9475118317ee57192021-11-18T07:22:10ZDerivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.1932-620310.1371/journal.pone.0035338https://doaj.org/article/d76a11f8453a487d9475118317ee57192012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22514728/?tool=EBIhttps://doaj.org/toc/1932-6203There is increasing evidence that breast and other cancers originate from and are maintained by a small fraction of stem/progenitor cells with self-renewal properties. Recent molecular profiling has identified six major subtypes of breast cancer: basal-like, ErbB2-overexpressing, normal breast epithelial-like, luminal A and B, and claudin-low subtypes. To help understand the relationship among mammary stem/progenitor cells and breast cancer subtypes, we have recently derived distinct hTERT-immortalized human mammary stem/progenitor cell lines: a K5(+)/K19(-) type, and a K5(+)/K19(+) type. Under specific culture conditions, bipotent K5(+)/K19(-) stem/progenitor cells differentiated into stable clonal populations that were K5(-)/K19(-) and exhibit self-renewal and unipotent myoepithelial differentiation potential in contrast to the parental K5(+)/K19(-) cells which are bipotent. These K5(-)/K19(-) cells function as myoepithelial progenitor cells and constitutively express markers of an epithelial to mesenchymal transition (EMT) and show high invasive and migratory abilities. In addition, these cells express a microarray signature of claudin-low breast cancers. The EMT characteristics of an un-transformed unipotent mammary myoepithelial progenitor cells together with claudin-low signature suggests that the claudin-low breast cancer subtype may arise from myoepithelial lineage committed progenitors. Availability of immortal MPCs should allow a more definitive analysis of their potential to give rise to claudin-low breast cancer subtype and facilitate biological and molecular/biochemical studies of this disease.Xiangshan ZhaoGautam K MalhotraHamid BandVimla BandPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35338 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangshan Zhao Gautam K Malhotra Hamid Band Vimla Band Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
description	There is increasing evidence that breast and other cancers originate from and are maintained by a small fraction of stem/progenitor cells with self-renewal properties. Recent molecular profiling has identified six major subtypes of breast cancer: basal-like, ErbB2-overexpressing, normal breast epithelial-like, luminal A and B, and claudin-low subtypes. To help understand the relationship among mammary stem/progenitor cells and breast cancer subtypes, we have recently derived distinct hTERT-immortalized human mammary stem/progenitor cell lines: a K5(+)/K19(-) type, and a K5(+)/K19(+) type. Under specific culture conditions, bipotent K5(+)/K19(-) stem/progenitor cells differentiated into stable clonal populations that were K5(-)/K19(-) and exhibit self-renewal and unipotent myoepithelial differentiation potential in contrast to the parental K5(+)/K19(-) cells which are bipotent. These K5(-)/K19(-) cells function as myoepithelial progenitor cells and constitutively express markers of an epithelial to mesenchymal transition (EMT) and show high invasive and migratory abilities. In addition, these cells express a microarray signature of claudin-low breast cancers. The EMT characteristics of an un-transformed unipotent mammary myoepithelial progenitor cells together with claudin-low signature suggests that the claudin-low breast cancer subtype may arise from myoepithelial lineage committed progenitors. Availability of immortal MPCs should allow a more definitive analysis of their potential to give rise to claudin-low breast cancer subtype and facilitate biological and molecular/biochemical studies of this disease.
format	article
author	Xiangshan Zhao Gautam K Malhotra Hamid Band Vimla Band
author_facet	Xiangshan Zhao Gautam K Malhotra Hamid Band Vimla Band
author_sort	Xiangshan Zhao
title	Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
title_short	Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
title_full	Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
title_fullStr	Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
title_full_unstemmed	Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
title_sort	derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d76a11f8453a487d9475118317ee5719
work_keys_str_mv	AT xiangshanzhao derivationofmyoepithelialprogenitorcellsfrombipotentmammarystemprogenitorcells AT gautamkmalhotra derivationofmyoepithelialprogenitorcellsfrombipotentmammarystemprogenitorcells AT hamidband derivationofmyoepithelialprogenitorcellsfrombipotentmammarystemprogenitorcells AT vimlaband derivationofmyoepithelialprogenitorcellsfrombipotentmammarystemprogenitorcells
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Derivation of myoepithelial progenitor cells from bipotent mammary stem/progenitor cells.

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