Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing

Abstract Although the wound healing effects of nitric oxide (NO) are known, the mechanism by which NO modulates corneal wound healing remains unclear. In this study, we investigated the effect of exogenous NO donor (NaNO2) on corneal wound healing. We found that NaNO2 (0.1 μM to 100 μM) increased hu...

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Autores principales:	Joo-Hee Park, Ja-Yeon Kim, Dong Ju Kim, Martha Kim, Minwook Chang, Roy S. Chuck, Choul Yong Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d76c32beac364cada601b8d529653f64
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spelling	oai:doaj.org-article:d76c32beac364cada601b8d529653f642021-12-02T11:41:11ZEffect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing10.1038/s41598-017-08576-92045-2322https://doaj.org/article/d76c32beac364cada601b8d529653f642017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08576-9https://doaj.org/toc/2045-2322Abstract Although the wound healing effects of nitric oxide (NO) are known, the mechanism by which NO modulates corneal wound healing remains unclear. In this study, we investigated the effect of exogenous NO donor (NaNO2) on corneal wound healing. We found that NaNO2 (0.1 μM to 100 μM) increased human corneal epithelial cell (HCEC) viability and migration. It also modulated the phosphorylation of mitogen-activated protein kinases (MAPKs) in a time- dependent manner in those HCECs. Further, p38 MAPK phosphorylation increased at 6 h and normalized at 24 h, while the phosphorylation of extracellular signal regulated kinase (ERK) was increased both at 6 h and 24 h. Topical treatment with NaNO2 (10 μM) enhanced corneal epithelial healing and decreased corneal opacity in murine corneal alkali burn model by modulating inflammatory cytokines. Our findings suggest that NO increased HCEC proliferation and migration via time-dependent MAPK activation and eventually enhanced corneal recovery from the alkali burn.Joo-Hee ParkJa-Yeon KimDong Ju KimMartha KimMinwook ChangRoy S. ChuckChoul Yong ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joo-Hee Park Ja-Yeon Kim Dong Ju Kim Martha Kim Minwook Chang Roy S. Chuck Choul Yong Park Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
description	Abstract Although the wound healing effects of nitric oxide (NO) are known, the mechanism by which NO modulates corneal wound healing remains unclear. In this study, we investigated the effect of exogenous NO donor (NaNO2) on corneal wound healing. We found that NaNO2 (0.1 μM to 100 μM) increased human corneal epithelial cell (HCEC) viability and migration. It also modulated the phosphorylation of mitogen-activated protein kinases (MAPKs) in a time- dependent manner in those HCECs. Further, p38 MAPK phosphorylation increased at 6 h and normalized at 24 h, while the phosphorylation of extracellular signal regulated kinase (ERK) was increased both at 6 h and 24 h. Topical treatment with NaNO2 (10 μM) enhanced corneal epithelial healing and decreased corneal opacity in murine corneal alkali burn model by modulating inflammatory cytokines. Our findings suggest that NO increased HCEC proliferation and migration via time-dependent MAPK activation and eventually enhanced corneal recovery from the alkali burn.
format	article
author	Joo-Hee Park Ja-Yeon Kim Dong Ju Kim Martha Kim Minwook Chang Roy S. Chuck Choul Yong Park
author_facet	Joo-Hee Park Ja-Yeon Kim Dong Ju Kim Martha Kim Minwook Chang Roy S. Chuck Choul Yong Park
author_sort	Joo-Hee Park
title	Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
title_short	Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
title_full	Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
title_fullStr	Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
title_full_unstemmed	Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing
title_sort	effect of nitric oxide on human corneal epithelial cell viability and corneal wound healing
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d76c32beac364cada601b8d529653f64
work_keys_str_mv	AT jooheepark effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT jayeonkim effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT dongjukim effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT marthakim effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT minwookchang effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT royschuck effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing AT choulyongpark effectofnitricoxideonhumancornealepithelialcellviabilityandcornealwoundhealing
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Effect of Nitric Oxide on Human Corneal Epithelial Cell Viability and Corneal Wound Healing

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