Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland

Abstract The development of ductal structures during branching morphogenesis relies on signals that specify ductal progenitors to set up a pattern for the ductal network. Here, we identify cellular asymmetries defined by the F-actin cytoskeleton and the cell adhesion protein ZO-1 as the earliest det...

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Autores principales:	Janice L. Walker, Weihao Wang, Edith Lin, Alison Romisher, Meghan P. Bouchie, Brigid Bleaken, A. Sue Menko, Maria A. Kukuruzinska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d770138b20474128aeea4295fea71d22
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spelling	oai:doaj.org-article:d770138b20474128aeea4295fea71d222021-12-02T14:01:24ZSpecification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland10.1038/s41598-020-79650-y2045-2322https://doaj.org/article/d770138b20474128aeea4295fea71d222021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79650-yhttps://doaj.org/toc/2045-2322Abstract The development of ductal structures during branching morphogenesis relies on signals that specify ductal progenitors to set up a pattern for the ductal network. Here, we identify cellular asymmetries defined by the F-actin cytoskeleton and the cell adhesion protein ZO-1 as the earliest determinants of duct specification in the embryonic submandibular gland (SMG). Apical polarity protein aPKCζ is then recruited to the sites of asymmetry in a ZO-1-dependent manner and collaborates with ROCK signaling to set up apical-basal polarity of ductal progenitors and further define the path of duct specification. Moreover, the motor protein myosin IIB, a mediator of mechanical force transmission along actin filaments, becomes localized to vertices linking the apical domains of multiple ductal epithelial cells during the formation of ductal lumens and drives duct maturation. These studies identify cytoskeletal, junctional and polarity proteins as the early determinants of duct specification and the patterning of a ductal tree during branching morphogenesis of the SMG.Janice L. WalkerWeihao WangEdith LinAlison RomisherMeghan P. BouchieBrigid BleakenA. Sue MenkoMaria A. KukuruzinskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janice L. Walker Weihao Wang Edith Lin Alison Romisher Meghan P. Bouchie Brigid Bleaken A. Sue Menko Maria A. Kukuruzinska Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
description	Abstract The development of ductal structures during branching morphogenesis relies on signals that specify ductal progenitors to set up a pattern for the ductal network. Here, we identify cellular asymmetries defined by the F-actin cytoskeleton and the cell adhesion protein ZO-1 as the earliest determinants of duct specification in the embryonic submandibular gland (SMG). Apical polarity protein aPKCζ is then recruited to the sites of asymmetry in a ZO-1-dependent manner and collaborates with ROCK signaling to set up apical-basal polarity of ductal progenitors and further define the path of duct specification. Moreover, the motor protein myosin IIB, a mediator of mechanical force transmission along actin filaments, becomes localized to vertices linking the apical domains of multiple ductal epithelial cells during the formation of ductal lumens and drives duct maturation. These studies identify cytoskeletal, junctional and polarity proteins as the early determinants of duct specification and the patterning of a ductal tree during branching morphogenesis of the SMG.
format	article
author	Janice L. Walker Weihao Wang Edith Lin Alison Romisher Meghan P. Bouchie Brigid Bleaken A. Sue Menko Maria A. Kukuruzinska
author_facet	Janice L. Walker Weihao Wang Edith Lin Alison Romisher Meghan P. Bouchie Brigid Bleaken A. Sue Menko Maria A. Kukuruzinska
author_sort	Janice L. Walker
title	Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
title_short	Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
title_full	Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
title_fullStr	Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
title_full_unstemmed	Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
title_sort	specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d770138b20474128aeea4295fea71d22
work_keys_str_mv	AT janicelwalker specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT weihaowang specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT edithlin specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT alisonromisher specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT meghanpbouchie specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT brigidbleaken specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT asuemenko specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland AT mariaakukuruzinska specificationofthepatterningofaductaltreeduringbranchingmorphogenesisofthesubmandibulargland
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Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland

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