Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination

The <i>Aspergillus niger</i> exometabolome was recently investigated using advanced gas chromatography in tandem with multivariate analysis, which allowed a metabolite biomarker pattern to be proposed. Microbial metabolomics patterns have gained enormous relevance, mainly due to the amou...

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Autores principales:	Joao Raul Belinato, Carina Pedrosa Costa, Adelaide Almeida, Silvia M. Rocha, Fabio Augusto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aspergillus niger</i> food contamination GC×GC metabolites HS-SPME Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/d777695ccc5c43b08ffb2082a6986267
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spelling	oai:doaj.org-article:d777695ccc5c43b08ffb2082a69862672021-11-25T17:36:43ZMapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination10.3390/foods101128702304-8158https://doaj.org/article/d777695ccc5c43b08ffb2082a69862672021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2870https://doaj.org/toc/2304-8158The <i>Aspergillus niger</i> exometabolome was recently investigated using advanced gas chromatography in tandem with multivariate analysis, which allowed a metabolite biomarker pattern to be proposed. Microbial metabolomics patterns have gained enormous relevance, mainly due to the amount of information made available, which may be useful in countless processes. One of the great challenges in microbial metabolomics is related to applications in more complex systems of metabolomics information obtained from studies carried out in culture media, as complications may occur due to the dynamic nature of biological systems. Thus, the main objective of this research was to evaluate the applicability of the <i>A. niger</i> metabololite biomarkers pattern for in situ and early evaluation of table grapes contamination, used as study model. <i>A. niger</i> is a ubiquitous fungus responsible for food contamination, being reported as one of the main agents of the black mold disease, a serious post-harvest pathology of table grapes. This work included analysis from 1 day of growth time of pure <i>A. niger</i> cultures, <i>A. niger</i> cultures obtained from previously contaminated grapes, and finally, an in situ solid-phase microextraction (SPME) approach directly on previously contaminated table grapes. Supervised multivariate analysis was performed which revealed that after 1 day of inoculation it was possible to detect <i>A. niger</i> biomarkers, which can be extremely useful in making this type of method possible for the rapid detection of food contamination. The results obtained confirm the potential applicability of the pattern of <i>A. niger</i> biomarkers for early detection of the fungi (after 1 day of contamination), and may be further explored for access food susceptibility to fungi contamination, based on direct analysis of the food item.Joao Raul BelinatoCarina Pedrosa CostaAdelaide AlmeidaSilvia M. RochaFabio AugustoMDPI AGarticle<i>Aspergillus niger</i>food contaminationGC×GCmetabolitesHS-SPMEChemical technologyTP1-1185ENFoods, Vol 10, Iss 2870, p 2870 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aspergillus niger</i> food contamination GC×GC metabolites HS-SPME Chemical technology TP1-1185
spellingShingle	<i>Aspergillus niger</i> food contamination GC×GC metabolites HS-SPME Chemical technology TP1-1185 Joao Raul Belinato Carina Pedrosa Costa Adelaide Almeida Silvia M. Rocha Fabio Augusto Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
description	The <i>Aspergillus niger</i> exometabolome was recently investigated using advanced gas chromatography in tandem with multivariate analysis, which allowed a metabolite biomarker pattern to be proposed. Microbial metabolomics patterns have gained enormous relevance, mainly due to the amount of information made available, which may be useful in countless processes. One of the great challenges in microbial metabolomics is related to applications in more complex systems of metabolomics information obtained from studies carried out in culture media, as complications may occur due to the dynamic nature of biological systems. Thus, the main objective of this research was to evaluate the applicability of the <i>A. niger</i> metabololite biomarkers pattern for in situ and early evaluation of table grapes contamination, used as study model. <i>A. niger</i> is a ubiquitous fungus responsible for food contamination, being reported as one of the main agents of the black mold disease, a serious post-harvest pathology of table grapes. This work included analysis from 1 day of growth time of pure <i>A. niger</i> cultures, <i>A. niger</i> cultures obtained from previously contaminated grapes, and finally, an in situ solid-phase microextraction (SPME) approach directly on previously contaminated table grapes. Supervised multivariate analysis was performed which revealed that after 1 day of inoculation it was possible to detect <i>A. niger</i> biomarkers, which can be extremely useful in making this type of method possible for the rapid detection of food contamination. The results obtained confirm the potential applicability of the pattern of <i>A. niger</i> biomarkers for early detection of the fungi (after 1 day of contamination), and may be further explored for access food susceptibility to fungi contamination, based on direct analysis of the food item.
format	article
author	Joao Raul Belinato Carina Pedrosa Costa Adelaide Almeida Silvia M. Rocha Fabio Augusto
author_facet	Joao Raul Belinato Carina Pedrosa Costa Adelaide Almeida Silvia M. Rocha Fabio Augusto
author_sort	Joao Raul Belinato
title	Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
title_short	Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
title_full	Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
title_fullStr	Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
title_full_unstemmed	Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination
title_sort	mapping <i>aspergillus niger</i> metabolite biomarkers for in situ and early evaluation of table grapes contamination
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d777695ccc5c43b08ffb2082a6986267
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Mapping <i>Aspergillus niger</i> Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination

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