In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier

Todor A Popov,1 Jean Emberlin,2 Peter Josling,3 Alexander Seifalian4 1University Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia, Bulgaria; 2Allergy UK, Sidcup, Kent, UK; 3Herbal Research Centre, Battle, East Sussex, UK; 4NanoRegMed Ltd, BioScience Innovation Centre, London, UKC...

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Autores principales:	Popov TA, Emberlin J, Josling P, Seifalian A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cellulose hydroxypropylmethylcellulose hpmc micronized powder inhaled allergens allergen barrier particulate matter 2.5 pm2.5 safety in rats. Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/d77a29e83d174d95b89d7d5615bbdd8a
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spelling	oai:doaj.org-article:d77a29e83d174d95b89d7d5615bbdd8a2021-12-02T08:06:45ZIn vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier1179-1470https://doaj.org/article/d77a29e83d174d95b89d7d5615bbdd8a2020-03-01T00:00:00Zhttps://www.dovepress.com/in-vitro-and-in-vivo-evaluation-of-the-efficacy-and-safety-of-powder-h-peer-reviewed-article-MDERhttps://doaj.org/toc/1179-1470Todor A Popov,1 Jean Emberlin,2 Peter Josling,3 Alexander Seifalian4 1University Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia, Bulgaria; 2Allergy UK, Sidcup, Kent, UK; 3Herbal Research Centre, Battle, East Sussex, UK; 4NanoRegMed Ltd, BioScience Innovation Centre, London, UKCorrespondence: Todor A PopovUniversity Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia 1612, BulgariaTel +359 2 451 9438Email ted.popov@gmail.comIntroduction: Insufflation of powder hydroxypropylmethylcellulose (pHPMC) in the nose has been proven an effective barrier in subjects with rhinitis in many clinical studies. We conducted additionally in vitro and in vivo experiments to address outstanding efficacy and safety issues.Methods: We used an experimental setup to demonstrate the inhibition of the diffusion of allergen extracts (house dust mite, Japanese cedar, Ragweed, Timothy grass) and pollutants (particulate matter 2.5 μm, PM2.5). Safety of pHPMC when insufflated in the airways of rats was assessed in 24 animals which were sacrificed; tissue sections from lungs, brain and liver were made 1, 24 and 48 hrs after pHPMC inhalation and compared to those of control animals.Results: pHPMC acted as an effective barrier to diffusion of both the liquid allergen extracts and of PM2.5 into the agar covered slides: the quantities of the other tested allergens ranged between < 0.5% and 14% of the quantities diffused in the void slides after 6 hrs. The quantity of PM2.5 penetrating the agar was reduced by 94%. Histological photomicrographs did not reveal any evidence of inflammation at 1, 24 and 48 hrs after pHPMC insufflation.Conclusion: Use of pHPMC should be viewed as a barrier enforcing measure against inhalatory ambient intruders.Keywords: cellulose, hydroxypropylmethylcellulose, HPMC, micronized powder, inhaled allergens, allergen barrier, particulate matter 2.5, PM2.5, safety in ratsPopov TAEmberlin JJosling PSeifalian ADove Medical Pressarticlecellulosehydroxypropylmethylcellulosehpmcmicronized powderinhaled allergensallergen barrierparticulate matter 2.5pm2.5safety in rats.Medical technologyR855-855.5ENMedical Devices: Evidence and Research, Vol Volume 13, Pp 107-113 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulose hydroxypropylmethylcellulose hpmc micronized powder inhaled allergens allergen barrier particulate matter 2.5 pm2.5 safety in rats. Medical technology R855-855.5
spellingShingle	cellulose hydroxypropylmethylcellulose hpmc micronized powder inhaled allergens allergen barrier particulate matter 2.5 pm2.5 safety in rats. Medical technology R855-855.5 Popov TA Emberlin J Josling P Seifalian A In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
description	Todor A Popov,1 Jean Emberlin,2 Peter Josling,3 Alexander Seifalian4 1University Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia, Bulgaria; 2Allergy UK, Sidcup, Kent, UK; 3Herbal Research Centre, Battle, East Sussex, UK; 4NanoRegMed Ltd, BioScience Innovation Centre, London, UKCorrespondence: Todor A PopovUniversity Hospital Sv. Ivan Rilski, Department of Occupational Diseases, Sofia 1612, BulgariaTel +359 2 451 9438Email ted.popov@gmail.comIntroduction: Insufflation of powder hydroxypropylmethylcellulose (pHPMC) in the nose has been proven an effective barrier in subjects with rhinitis in many clinical studies. We conducted additionally in vitro and in vivo experiments to address outstanding efficacy and safety issues.Methods: We used an experimental setup to demonstrate the inhibition of the diffusion of allergen extracts (house dust mite, Japanese cedar, Ragweed, Timothy grass) and pollutants (particulate matter 2.5 μm, PM2.5). Safety of pHPMC when insufflated in the airways of rats was assessed in 24 animals which were sacrificed; tissue sections from lungs, brain and liver were made 1, 24 and 48 hrs after pHPMC inhalation and compared to those of control animals.Results: pHPMC acted as an effective barrier to diffusion of both the liquid allergen extracts and of PM2.5 into the agar covered slides: the quantities of the other tested allergens ranged between < 0.5% and 14% of the quantities diffused in the void slides after 6 hrs. The quantity of PM2.5 penetrating the agar was reduced by 94%. Histological photomicrographs did not reveal any evidence of inflammation at 1, 24 and 48 hrs after pHPMC insufflation.Conclusion: Use of pHPMC should be viewed as a barrier enforcing measure against inhalatory ambient intruders.Keywords: cellulose, hydroxypropylmethylcellulose, HPMC, micronized powder, inhaled allergens, allergen barrier, particulate matter 2.5, PM2.5, safety in rats
format	article
author	Popov TA Emberlin J Josling P Seifalian A
author_facet	Popov TA Emberlin J Josling P Seifalian A
author_sort	Popov TA
title	In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
title_short	In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
title_full	In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
title_fullStr	In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
title_full_unstemmed	In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier
title_sort	in vitro and in vivo evaluation of the efficacy and safety of powder hydroxypropylmethylcellulose as nasal mucosal barrier
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/d77a29e83d174d95b89d7d5615bbdd8a
work_keys_str_mv	AT popovta invitroandinvivoevaluationoftheefficacyandsafetyofpowderhydroxypropylmethylcelluloseasnasalmucosalbarrier AT emberlinj invitroandinvivoevaluationoftheefficacyandsafetyofpowderhydroxypropylmethylcelluloseasnasalmucosalbarrier AT joslingp invitroandinvivoevaluationoftheefficacyandsafetyofpowderhydroxypropylmethylcelluloseasnasalmucosalbarrier AT seifaliana invitroandinvivoevaluationoftheefficacyandsafetyofpowderhydroxypropylmethylcelluloseasnasalmucosalbarrier
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In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier

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