Immune cell profiling in cancer: molecular approaches to cell-specific identification

Abstract The immune system has many important regulatory roles in cancer development and progression. Given the emergence of effective immune therapies against many cancers, reliable predictors of response are needed. One method of determining response is by evaluating immune cell populations from t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yasmin A. Lyons, Sherry Y. Wu, Willem W. Overwijk, Keith A. Baggerly, Anil K. Sood
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d783f7cb41ea43a2bccc486e2cbc8a9b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d783f7cb41ea43a2bccc486e2cbc8a9b
record_format	dspace
spelling	oai:doaj.org-article:d783f7cb41ea43a2bccc486e2cbc8a9b2021-12-02T13:41:45ZImmune cell profiling in cancer: molecular approaches to cell-specific identification10.1038/s41698-017-0031-02397-768Xhttps://doaj.org/article/d783f7cb41ea43a2bccc486e2cbc8a9b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41698-017-0031-0https://doaj.org/toc/2397-768XAbstract The immune system has many important regulatory roles in cancer development and progression. Given the emergence of effective immune therapies against many cancers, reliable predictors of response are needed. One method of determining response is by evaluating immune cell populations from treated and untreated tumor samples. The amount of material obtained from tumor biopsies can be limited; therefore, gene-based or protein-based analyses may be attractive because they require minimal tissue. Cell-specific signatures are being analyzed with use of the latest technologies, including NanoString’s nCounter technology, intracellular staining flow cytometry, cytometry by time-of-flight, RNA-Seq, and barcoding antibody-based protein arrays. These signatures provide information about the contributions of specific types of immune cells to bulk tumor samples. To date, both tumor tissue and immune cells have been analyzed for molecular expression profiles that can assess genes and proteins that are specific to immune cells, yielding results of varying specificity. Here, we discuss the importance of profiling tumor tissue and immune cells to identify immune-cell-associated genes and proteins and specific gene profiles of immune cells. We also discuss the use of these signatures in cancer treatment and the challenges faced in molecular expression profiling of immune cell populations.Yasmin A. LyonsSherry Y. WuWillem W. OverwijkKeith A. BaggerlyAnil K. SoodNature PortfolioarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Precision Oncology, Vol 1, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Yasmin A. Lyons Sherry Y. Wu Willem W. Overwijk Keith A. Baggerly Anil K. Sood Immune cell profiling in cancer: molecular approaches to cell-specific identification
description	Abstract The immune system has many important regulatory roles in cancer development and progression. Given the emergence of effective immune therapies against many cancers, reliable predictors of response are needed. One method of determining response is by evaluating immune cell populations from treated and untreated tumor samples. The amount of material obtained from tumor biopsies can be limited; therefore, gene-based or protein-based analyses may be attractive because they require minimal tissue. Cell-specific signatures are being analyzed with use of the latest technologies, including NanoString’s nCounter technology, intracellular staining flow cytometry, cytometry by time-of-flight, RNA-Seq, and barcoding antibody-based protein arrays. These signatures provide information about the contributions of specific types of immune cells to bulk tumor samples. To date, both tumor tissue and immune cells have been analyzed for molecular expression profiles that can assess genes and proteins that are specific to immune cells, yielding results of varying specificity. Here, we discuss the importance of profiling tumor tissue and immune cells to identify immune-cell-associated genes and proteins and specific gene profiles of immune cells. We also discuss the use of these signatures in cancer treatment and the challenges faced in molecular expression profiling of immune cell populations.
format	article
author	Yasmin A. Lyons Sherry Y. Wu Willem W. Overwijk Keith A. Baggerly Anil K. Sood
author_facet	Yasmin A. Lyons Sherry Y. Wu Willem W. Overwijk Keith A. Baggerly Anil K. Sood
author_sort	Yasmin A. Lyons
title	Immune cell profiling in cancer: molecular approaches to cell-specific identification
title_short	Immune cell profiling in cancer: molecular approaches to cell-specific identification
title_full	Immune cell profiling in cancer: molecular approaches to cell-specific identification
title_fullStr	Immune cell profiling in cancer: molecular approaches to cell-specific identification
title_full_unstemmed	Immune cell profiling in cancer: molecular approaches to cell-specific identification
title_sort	immune cell profiling in cancer: molecular approaches to cell-specific identification
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d783f7cb41ea43a2bccc486e2cbc8a9b
work_keys_str_mv	AT yasminalyons immunecellprofilingincancermolecularapproachestocellspecificidentification AT sherryywu immunecellprofilingincancermolecularapproachestocellspecificidentification AT willemwoverwijk immunecellprofilingincancermolecularapproachestocellspecificidentification AT keithabaggerly immunecellprofilingincancermolecularapproachestocellspecificidentification AT anilksood immunecellprofilingincancermolecularapproachestocellspecificidentification
_version_	1718392584314814464

Immune cell profiling in cancer: molecular approaches to cell-specific identification

Ejemplares similares