Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside

Abstract Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd,...

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Autores principales:	Amer H. Asseri, Albert Godoy-Hernandez, Hojjat Ghasemi Goojani, Holger Lill, Junshi Sakamoto, Duncan G. G. McMillan, Dirk Bald
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7861c6cfd05454c9ce5e73106409e83
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spelling	oai:doaj.org-article:d7861c6cfd05454c9ce5e73106409e832021-12-02T14:27:53ZCardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside10.1038/s41598-021-87354-02045-2322https://doaj.org/article/d7861c6cfd05454c9ce5e73106409e832021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87354-0https://doaj.org/toc/2045-2322Abstract Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent K M value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo 3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo 3.Amer H. AsseriAlbert Godoy-HernandezHojjat Ghasemi GoojaniHolger LillJunshi SakamotoDuncan G. G. McMillanDirk BaldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amer H. Asseri Albert Godoy-Hernandez Hojjat Ghasemi Goojani Holger Lill Junshi Sakamoto Duncan G. G. McMillan Dirk Bald Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
description	Abstract Cardiolipin (CL) is a lipid that is found in the membranes of bacteria and the inner membranes of mitochondria. CL can increase the activity of integral membrane proteins, in particular components of respiratory pathways. We here report that CL activated detergent-solubilized cytochrome bd, a terminal oxidase from Escherichia coli. CL enhanced the oxygen consumption activity ~ twofold and decreased the apparent K M value for ubiquinol-1 as substrate from 95 µM to 35 µM. Activation by CL was also observed for cytochrome bd from two Gram-positive species, Geobacillus thermodenitrificans and Corynebacterium glutamicum, and for cytochrome bo 3 from E. coli. Taken together, CL can enhance the activity of detergent-solubilized cytochrome bd and cytochrome bo 3.
format	article
author	Amer H. Asseri Albert Godoy-Hernandez Hojjat Ghasemi Goojani Holger Lill Junshi Sakamoto Duncan G. G. McMillan Dirk Bald
author_facet	Amer H. Asseri Albert Godoy-Hernandez Hojjat Ghasemi Goojani Holger Lill Junshi Sakamoto Duncan G. G. McMillan Dirk Bald
author_sort	Amer H. Asseri
title	Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
title_short	Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
title_full	Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
title_fullStr	Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
title_full_unstemmed	Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
title_sort	cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7861c6cfd05454c9ce5e73106409e83
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Cardiolipin enhances the enzymatic activity of cytochrome bd and cytochrome bo 3 solubilized in dodecyl-maltoside

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