Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.

In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associat...

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Autores principales:	Hidenori Tani, Yasuko Onuma, Yuzuru Ito, Masaki Torimura
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d789dca9080f4695ab247ba5adb92bec
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spelling	oai:doaj.org-article:d789dca9080f4695ab247ba5adb92bec2021-11-25T06:02:44ZLong non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.1932-620310.1371/journal.pone.0106282https://doaj.org/article/d789dca9080f4695ab247ba5adb92bec2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25171338/?tool=EBIhttps://doaj.org/toc/1932-6203In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model chemical stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs.Hidenori TaniYasuko OnumaYuzuru ItoMasaki TorimuraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e106282 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hidenori Tani Yasuko Onuma Yuzuru Ito Masaki Torimura Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
description	In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified six novel lncRNAs (CDKN2B-AS1, MIR22HG, GABPB1-AS1, FLJ33630, LINC00152, and LINC0541471_v2) that respond to model chemical stresses (cycloheximide, hydrogen peroxide, cadmium, or arsenic) in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. Compared with typical mRNAs such as p53-related mRNAs, the lncRNAs highly and rapidly responded to chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs.
format	article
author	Hidenori Tani Yasuko Onuma Yuzuru Ito Masaki Torimura
author_facet	Hidenori Tani Yasuko Onuma Yuzuru Ito Masaki Torimura
author_sort	Hidenori Tani
title	Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
title_short	Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
title_full	Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
title_fullStr	Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
title_full_unstemmed	Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
title_sort	long non-coding rnas as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d789dca9080f4695ab247ba5adb92bec
work_keys_str_mv	AT hidenoritani longnoncodingrnasassurrogateindicatorsforchemicalstressresponsesinhumaninducedpluripotentstemcells AT yasukoonuma longnoncodingrnasassurrogateindicatorsforchemicalstressresponsesinhumaninducedpluripotentstemcells AT yuzuruito longnoncodingrnasassurrogateindicatorsforchemicalstressresponsesinhumaninducedpluripotentstemcells AT masakitorimura longnoncodingrnasassurrogateindicatorsforchemicalstressresponsesinhumaninducedpluripotentstemcells
_version_	1718414250477617152

Long non-coding RNAs as surrogate indicators for chemical stress responses in human-induced pluripotent stem cells.

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