Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications

Coating the surfaces of implantable materials with various active principles to ensure inhibition of microbial adhesion, is a solution to reduce infections associated with dental implant. The aim of the study was to optimize the polydopamine films coating on the Ti-6Al-6V alloy surface in order to o...

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Autores principales:	Ioana Cristina Marinas, Bianca Maria Tihauan, Andreea Gabriela Diaconu, Xenia Filip, Anca Petran, Ioana-Georgeta Grosu, Diana Bogdan, Lucian Barbu, Ana Maria Ivanof, Marin Angheloiu, Grațiela Gradisteanu Pircalabioru, Claudiu Filip
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	polydopamine antibiofilm activity cytotoxicity Ti-6Al-4V alloy Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/d78b113e0df94874b22d24fcfeedd2a8
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spelling	oai:doaj.org-article:d78b113e0df94874b22d24fcfeedd2a82021-11-25T17:16:42ZPolydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications10.3390/coatings111113852079-6412https://doaj.org/article/d78b113e0df94874b22d24fcfeedd2a82021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6412/11/11/1385https://doaj.org/toc/2079-6412Coating the surfaces of implantable materials with various active principles to ensure inhibition of microbial adhesion, is a solution to reduce infections associated with dental implant. The aim of the study was to optimize the polydopamine films coating on the Ti-6Al-6V alloy surface in order to obtain a maximum of antimicrobial/antibiofilm efficacy and reduced cytotoxicity. Surface characterization was performed by evaluating the morphology (SEM, AFM) and structures (Solid-state 13C NMR and EPR). Antimicrobial activity was assessed by logarithmic reduction of CFU/mL, and the antibiofilm activity by reducing the adhesion of <i>Escherichia coli, Staphylococcus</i> <i>aureus</i>, and <i>Candida albicans</i> strains. The release of NO was observed especially for <i>C. albicans</i> strain, which confirms the results obtained for microbial adhesion. Among the PDA coatings, for 0.45:0.88 (KMnO<sub>4</sub>:dopamine) molar ratio the optimal compromise was obtained in terms of antimicrobial activity and cytotoxicity, while the 0.1:1.5 ratio (KMnO<sub>4</sub>:dopamine) led to higher NO release and implicitly the reduction of the adhesion capacities only for <i>C. albicans</i>, being slightly cytotoxic but with moderate release of LDH. The proposed materials can be used to reduce the adhesion of yeast to the implantable material and thus inhibit the formation of microbial biofilms.Ioana Cristina MarinasBianca Maria TihauanAndreea Gabriela DiaconuXenia FilipAnca PetranIoana-Georgeta GrosuDiana BogdanLucian BarbuAna Maria IvanofMarin AngheloiuGrațiela Gradisteanu PircalabioruClaudiu FilipMDPI AGarticlepolydopamineantibiofilm activitycytotoxicityTi-6Al-4V alloyEngineering (General). Civil engineering (General)TA1-2040ENCoatings, Vol 11, Iss 1385, p 1385 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	polydopamine antibiofilm activity cytotoxicity Ti-6Al-4V alloy Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	polydopamine antibiofilm activity cytotoxicity Ti-6Al-4V alloy Engineering (General). Civil engineering (General) TA1-2040 Ioana Cristina Marinas Bianca Maria Tihauan Andreea Gabriela Diaconu Xenia Filip Anca Petran Ioana-Georgeta Grosu Diana Bogdan Lucian Barbu Ana Maria Ivanof Marin Angheloiu Grațiela Gradisteanu Pircalabioru Claudiu Filip Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
description	Coating the surfaces of implantable materials with various active principles to ensure inhibition of microbial adhesion, is a solution to reduce infections associated with dental implant. The aim of the study was to optimize the polydopamine films coating on the Ti-6Al-6V alloy surface in order to obtain a maximum of antimicrobial/antibiofilm efficacy and reduced cytotoxicity. Surface characterization was performed by evaluating the morphology (SEM, AFM) and structures (Solid-state 13C NMR and EPR). Antimicrobial activity was assessed by logarithmic reduction of CFU/mL, and the antibiofilm activity by reducing the adhesion of <i>Escherichia coli, Staphylococcus</i> <i>aureus</i>, and <i>Candida albicans</i> strains. The release of NO was observed especially for <i>C. albicans</i> strain, which confirms the results obtained for microbial adhesion. Among the PDA coatings, for 0.45:0.88 (KMnO<sub>4</sub>:dopamine) molar ratio the optimal compromise was obtained in terms of antimicrobial activity and cytotoxicity, while the 0.1:1.5 ratio (KMnO<sub>4</sub>:dopamine) led to higher NO release and implicitly the reduction of the adhesion capacities only for <i>C. albicans</i>, being slightly cytotoxic but with moderate release of LDH. The proposed materials can be used to reduce the adhesion of yeast to the implantable material and thus inhibit the formation of microbial biofilms.
format	article
author	Ioana Cristina Marinas Bianca Maria Tihauan Andreea Gabriela Diaconu Xenia Filip Anca Petran Ioana-Georgeta Grosu Diana Bogdan Lucian Barbu Ana Maria Ivanof Marin Angheloiu Grațiela Gradisteanu Pircalabioru Claudiu Filip
author_facet	Ioana Cristina Marinas Bianca Maria Tihauan Andreea Gabriela Diaconu Xenia Filip Anca Petran Ioana-Georgeta Grosu Diana Bogdan Lucian Barbu Ana Maria Ivanof Marin Angheloiu Grațiela Gradisteanu Pircalabioru Claudiu Filip
author_sort	Ioana Cristina Marinas
title	Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
title_short	Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
title_full	Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
title_fullStr	Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
title_full_unstemmed	Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications
title_sort	polydopamine-assisted surface modification of ti-6al-4v alloy with anti-biofilm activity for dental implantology applications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d78b113e0df94874b22d24fcfeedd2a8
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Polydopamine-Assisted Surface Modification of Ti-6Al-4V Alloy with Anti-Biofilm Activity for Dental Implantology Applications

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