The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB

MafB proteins are toxins secreted by Neisseria spp. which are involved in interbacterial competition. Their secretion mechanism has so far not been elucidated. Each strain can produce several MafB variants. On the chromosome, the mafB genes are localized on genomic islands also containing mafA genes...

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Autores principales:	Jesús Arenas, Laura Catón, Tom van den Hoeven, Vincent de Maat, Juan Cruz Herrero, Jan Tommassen
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	interbacterial competition mafa mafb neisseria protein secretion secretome toxin Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/d78d6fcffd204bc1a4ddc09634334f27
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spelling	oai:doaj.org-article:d78d6fcffd204bc1a4ddc09634334f272021-11-17T14:21:59ZThe outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB2150-55942150-560810.1080/21505594.2020.1851940https://doaj.org/article/d78d6fcffd204bc1a4ddc09634334f272020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1851940https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608MafB proteins are toxins secreted by Neisseria spp. which are involved in interbacterial competition. Their secretion mechanism has so far not been elucidated. Each strain can produce several MafB variants. On the chromosome, the mafB genes are localized on genomic islands also containing mafA genes. MafA proteins have a role in virulence with reported activities in adhesion and transcytosis of pathogenic Neisseria, a priori unrelated to MafB activities. In this study, we investigated the possible involvement of MafA in the transport of MafB across the outer membrane of Neisseria meningitidis. In wild-type strains, proteolytic fragments of MafB proteins were detected in the extracellular medium. In the absence of MafA, secretion was abrogated, and, in the case of MafBI, full-length and truncated polypeptides were detected inside the cells and inside outer-membrane vesicles. MafBI secretion required its cognate MafA, whereas MafBIII could use any MafA. Heterologous expression in Escherichia coli showed that MafBIII is transported to a cell-surface-exposed, i.e. protease-accessible, location in a MafA-dependent way. MafA itself was found to be localized to the outer membrane, forming large oligomeric complexes. As homologs were found in diverse bacteria, the Maf system represents a new protein secretion system in Gram-negative bacteria.Jesús ArenasLaura CatónTom van den HoevenVincent de MaatJuan Cruz HerreroJan TommassenTaylor & Francis Grouparticleinterbacterial competitionmafamafbneisseriaprotein secretionsecretometoxinInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1701-1715 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	interbacterial competition mafa mafb neisseria protein secretion secretome toxin Infectious and parasitic diseases RC109-216
spellingShingle	interbacterial competition mafa mafb neisseria protein secretion secretome toxin Infectious and parasitic diseases RC109-216 Jesús Arenas Laura Catón Tom van den Hoeven Vincent de Maat Juan Cruz Herrero Jan Tommassen The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
description	MafB proteins are toxins secreted by Neisseria spp. which are involved in interbacterial competition. Their secretion mechanism has so far not been elucidated. Each strain can produce several MafB variants. On the chromosome, the mafB genes are localized on genomic islands also containing mafA genes. MafA proteins have a role in virulence with reported activities in adhesion and transcytosis of pathogenic Neisseria, a priori unrelated to MafB activities. In this study, we investigated the possible involvement of MafA in the transport of MafB across the outer membrane of Neisseria meningitidis. In wild-type strains, proteolytic fragments of MafB proteins were detected in the extracellular medium. In the absence of MafA, secretion was abrogated, and, in the case of MafBI, full-length and truncated polypeptides were detected inside the cells and inside outer-membrane vesicles. MafBI secretion required its cognate MafA, whereas MafBIII could use any MafA. Heterologous expression in Escherichia coli showed that MafBIII is transported to a cell-surface-exposed, i.e. protease-accessible, location in a MafA-dependent way. MafA itself was found to be localized to the outer membrane, forming large oligomeric complexes. As homologs were found in diverse bacteria, the Maf system represents a new protein secretion system in Gram-negative bacteria.
format	article
author	Jesús Arenas Laura Catón Tom van den Hoeven Vincent de Maat Juan Cruz Herrero Jan Tommassen
author_facet	Jesús Arenas Laura Catón Tom van den Hoeven Vincent de Maat Juan Cruz Herrero Jan Tommassen
author_sort	Jesús Arenas
title	The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
title_short	The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
title_full	The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
title_fullStr	The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
title_full_unstemmed	The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB
title_sort	outer-membrane protein mafa of neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein mafb
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/d78d6fcffd204bc1a4ddc09634334f27
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The outer-membrane protein MafA of Neisseria meningitidis constitutes a novel protein secretion pathway specific for the fratricide protein MafB

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