Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Abstract Background Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase produc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhi-Qing Peng, Chuang Li, Yi Lin, Sheng-Shan Wu, Li-Hui Gan, Jian Liu, Shu-Liang Yang, Xian-Hai Zeng, Lu Lin
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Trichoderma afroharzianum Compound mutagenesis Adaptive laboratory evolution Cellulase Biomass Fuel TP315-360 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/d791d4d031654280b77d86bbf18715e3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d791d4d031654280b77d86bbf18715e3
record_format	dspace
spelling	oai:doaj.org-article:d791d4d031654280b77d86bbf18715e32021-11-28T12:22:20ZCellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-1210.1186/s13068-021-02072-z1754-6834https://doaj.org/article/d791d4d031654280b77d86bbf18715e32021-11-01T00:00:00Zhttps://doi.org/10.1186/s13068-021-02072-zhttps://doaj.org/toc/1754-6834Abstract Background Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase production, which are far from meeting the requirements of industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy is an effective method to increase the production of fungal enzymes. Results This study obtained a mutant of Trichoderma afroharzianum by exposures to N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric and Room Temperature Plasma (ARTP) and ALE with high sugar stress. The T. afroharzianum mutant MEA-12 produced 0.60, 5.47, 0.31 and 2.17 IU/mL FPase, CMCase, pNPCase and pNPGase, respectively. These levels were 4.33, 6.37, 4.92 and 4.15 times higher than those of the parental strain, respectively. Also, it was found that T. afroharzianum had the same carbon catabolite repression (CCR) effect as other Trichoderma in liquid submerged fermentation. In contrast, the mutant MEA-12 can tolerate the inhibition of glucose (up to 20 mM) without affecting enzyme production under inducing conditions. Interestingly, crude enzyme from MEA-12 showed high enzymatic hydrolysis efficiency against three different biomasses (cornstalk, bamboo and reed), when combined with cellulase from T. reesei Rut-C30. In addition, the factors that improved cellulase production by MEA-12 were clarified. Conclusions Overall, compound mutagenesis combined with ALE effectively increased the production of fungal cellulase. A super-producing mutant MEA-12 was obtained, and its cellulase could hydrolyze common biomasses efficiently, in combination with enzymes derived from model strain T. reesei, which provides a new choice for processing of bioresources in the future.Zhi-Qing PengChuang LiYi LinSheng-Shan WuLi-Hui GanJian LiuShu-Liang YangXian-Hai ZengLu LinBMCarticleTrichoderma afroharzianumCompound mutagenesisAdaptive laboratory evolutionCellulaseBiomassFuelTP315-360BiotechnologyTP248.13-248.65ENBiotechnology for Biofuels, Vol 14, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trichoderma afroharzianum Compound mutagenesis Adaptive laboratory evolution Cellulase Biomass Fuel TP315-360 Biotechnology TP248.13-248.65
spellingShingle	Trichoderma afroharzianum Compound mutagenesis Adaptive laboratory evolution Cellulase Biomass Fuel TP315-360 Biotechnology TP248.13-248.65 Zhi-Qing Peng Chuang Li Yi Lin Sheng-Shan Wu Li-Hui Gan Jian Liu Shu-Liang Yang Xian-Hai Zeng Lu Lin Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
description	Abstract Background Cellulase plays a key role in converting cellulosic biomass into fermentable sugar to produce chemicals and fuels, which is generally produced by filamentous fungi. However, most of the filamentous fungi obtained by natural breeding have low secretory capacity in cellulase production, which are far from meeting the requirements of industrial production. Random mutagenesis combined with adaptive laboratory evolution (ALE) strategy is an effective method to increase the production of fungal enzymes. Results This study obtained a mutant of Trichoderma afroharzianum by exposures to N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), Ethyl Methanesulfonate (EMS), Atmospheric and Room Temperature Plasma (ARTP) and ALE with high sugar stress. The T. afroharzianum mutant MEA-12 produced 0.60, 5.47, 0.31 and 2.17 IU/mL FPase, CMCase, pNPCase and pNPGase, respectively. These levels were 4.33, 6.37, 4.92 and 4.15 times higher than those of the parental strain, respectively. Also, it was found that T. afroharzianum had the same carbon catabolite repression (CCR) effect as other Trichoderma in liquid submerged fermentation. In contrast, the mutant MEA-12 can tolerate the inhibition of glucose (up to 20 mM) without affecting enzyme production under inducing conditions. Interestingly, crude enzyme from MEA-12 showed high enzymatic hydrolysis efficiency against three different biomasses (cornstalk, bamboo and reed), when combined with cellulase from T. reesei Rut-C30. In addition, the factors that improved cellulase production by MEA-12 were clarified. Conclusions Overall, compound mutagenesis combined with ALE effectively increased the production of fungal cellulase. A super-producing mutant MEA-12 was obtained, and its cellulase could hydrolyze common biomasses efficiently, in combination with enzymes derived from model strain T. reesei, which provides a new choice for processing of bioresources in the future.
format	article
author	Zhi-Qing Peng Chuang Li Yi Lin Sheng-Shan Wu Li-Hui Gan Jian Liu Shu-Liang Yang Xian-Hai Zeng Lu Lin
author_facet	Zhi-Qing Peng Chuang Li Yi Lin Sheng-Shan Wu Li-Hui Gan Jian Liu Shu-Liang Yang Xian-Hai Zeng Lu Lin
author_sort	Zhi-Qing Peng
title	Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
title_short	Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
title_full	Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
title_fullStr	Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
title_full_unstemmed	Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12
title_sort	cellulase production and efficient saccharification of biomass by a new mutant trichoderma afroharzianum mea-12
publisher	BMC
publishDate	2021
url	https://doaj.org/article/d791d4d031654280b77d86bbf18715e3
work_keys_str_mv	AT zhiqingpeng cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT chuangli cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT yilin cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT shengshanwu cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT lihuigan cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT jianliu cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT shuliangyang cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT xianhaizeng cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12 AT lulin cellulaseproductionandefficientsaccharificationofbiomassbyanewmutanttrichodermaafroharzianummea12
_version_	1718408047712272384

Cellulase production and efficient saccharification of biomass by a new mutant Trichoderma afroharzianum MEA-12

Ejemplares similares