Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodont...

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Autores principales:	Anastasia V. Kotova, Arseniy A. Lobov, Julia A. Dombrovskaya, Valentina Y. Sannikova, Nadezhda A. Ryumina, Polina Klausen, Alexey L. Shavarda, Anna B. Malashicheva, Natella I. Enukashvily
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	dental stem cells dental pulp stem cells periodontal ligament stem cells osteogenic differentiation odontoblastic differentiation pluripotency Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d7926e8bbff2428596e5e73c3fb71c43
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spelling	oai:doaj.org-article:d7926e8bbff2428596e5e73c3fb71c432021-11-25T16:49:33ZComparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome10.3390/biomedicines91116062227-9059https://doaj.org/article/d7926e8bbff2428596e5e73c3fb71c432021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1606https://doaj.org/toc/2227-9059Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in <i>Nestin</i> (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. <i>OCT4</i> mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of <i>OCT4</i> mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. <i>RUNX2</i> mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. <i>DSPP</i> and <i>DMP1</i>, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products.Anastasia V. KotovaArseniy A. LobovJulia A. DombrovskayaValentina Y. SannikovaNadezhda A. RyuminaPolina KlausenAlexey L. ShavardaAnna B. MalashichevaNatella I. EnukashvilyMDPI AGarticledental stem cellsdental pulp stem cellsperiodontal ligament stem cellsosteogenic differentiationodontoblastic differentiationpluripotencyBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1606, p 1606 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dental stem cells dental pulp stem cells periodontal ligament stem cells osteogenic differentiation odontoblastic differentiation pluripotency Biology (General) QH301-705.5
spellingShingle	dental stem cells dental pulp stem cells periodontal ligament stem cells osteogenic differentiation odontoblastic differentiation pluripotency Biology (General) QH301-705.5 Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
description	Dental stem cells are heterogeneous in their properties. Despite their common origin from neural crest stem cells, they have different functional capacities and biological functions due to niche influence. In this study, we assessed the differences between dental pulp stem cells (DPSC) and periodontal ligament stem cells (PDLSC) in their pluripotency and neuroepithelial markers transcription, morphological and functional features, osteoblast/odontoblast differentiation and proteomic profile during osteogenic differentiation. The data were collected in paired observations: two cell cultures, DPSC and PDLSC, were obtained from each donor. Both populations had the mesenchymal stem cells surface marker set exposed on their membranes but differed in <i>Nestin</i> (a marker of neuroectodermal origin) expression, morphology, and proliferation rate. <i>OCT4</i> mRNA was revealed in DPSC and PDLSC, while OCT4 protein was present in the nuclei of DPSC only. However, transcription of <i>OCT4</i> mRNA was 1000–10,000-fold lower in dental stem cells than in blastocysts. DPSC proliferated at a slower rate and have a shape closer to polygonal but they responded better to osteogenic stimuli as compared to PDLSC. <i>RUNX2</i> mRNA was detected by qPCR in both types of dental stem cells but RUNX2 protein was detected by LC-MS/MS shotgun proteomics only in PDLSC suggesting the posttranscriptional regulation. <i>DSPP</i> and <i>DMP1</i>, marker genes of odontoblastic type of osteogenic differentiation, were transcribed in DPSC but not in PDLSC samples. Our results prove that DPSC and PDLSC are different in their biology and therapeutic potential: DPSC are a good candidate for osteogenic or odontogenic bone-replacement cell-seeded medicines, while fast proliferating PDLSC are a prospective candidate for other cell products.
format	article
author	Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily
author_facet	Anastasia V. Kotova Arseniy A. Lobov Julia A. Dombrovskaya Valentina Y. Sannikova Nadezhda A. Ryumina Polina Klausen Alexey L. Shavarda Anna B. Malashicheva Natella I. Enukashvily
author_sort	Anastasia V. Kotova
title	Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
title_short	Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
title_full	Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
title_fullStr	Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
title_full_unstemmed	Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome
title_sort	comparative analysis of dental pulp and periodontal stem cells: differences in morphology, functionality, osteogenic differentiation and proteome
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d7926e8bbff2428596e5e73c3fb71c43
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Comparative Analysis of Dental Pulp and Periodontal Stem Cells: Differences in Morphology, Functionality, Osteogenic Differentiation and Proteome

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