Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis

Abstract Pathogenic fungi including Penicillium digitatum and Penicillium italicum are the main destructive pathogens in the citrus industry, causing great losses during postharvest process. To our knowledge, only one mycovirus from P. digitatum has been reported, and the prevalence of such mycoviru...

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Autores principales:	Yuhui Niu, Yongze Yuan, Jiali Mao, Zhu Yang, Qianwen Cao, Tingfu Zhang, Shengqiang Wang, Deli Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7977fb1596b41f68eb1c26e553c9251
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spelling	oai:doaj.org-article:d7977fb1596b41f68eb1c26e553c92512021-12-02T15:08:10ZCharacterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis10.1038/s41598-018-23807-32045-2322https://doaj.org/article/d7977fb1596b41f68eb1c26e553c92512018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23807-3https://doaj.org/toc/2045-2322Abstract Pathogenic fungi including Penicillium digitatum and Penicillium italicum are the main destructive pathogens in the citrus industry, causing great losses during postharvest process. To our knowledge, only one mycovirus from P. digitatum has been reported, and the prevalence of such mycoviruses against citrus postharvest pathogenic fungi and their genotyping were still under investigation. In the present study, we showed that 39 of 152 Penicillium isolates from main citrus-growing areas in China were infected with various mycoviruses belonging to polymycoviruses, Narna-like viruses, and families Totiviridae, Partitivirdae and Chrysoviridae. The next generation sequencing (NGS) towards virus genome library and the following molecular analysis revealed two novel mycoviruses Penicillium digitatum polymycovirus 1 (PdPmV1) and Penicillium digitatum Narna-like virus 1 (PdNLV1), coexisting in P. digitatum strain HS-RH2. The fungicide-resistant P. digitatum strains HS-F6 and HS-E9 coinfected by PdPmV1 and PdNLV1 exhibited obvious reduction in triazole drug prochloraz resistance by mycelial growth analysis on both PDA plates and citrus fruit epidermis with given prochloraz concentration. This report at the first time characterized two novel mycoviruses from P. digitatum and revealed the mycovirus-induced reduction of fungicide resistance.Yuhui NiuYongze YuanJiali MaoZhu YangQianwen CaoTingfu ZhangShengqiang WangDeli LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuhui Niu Yongze Yuan Jiali Mao Zhu Yang Qianwen Cao Tingfu Zhang Shengqiang Wang Deli Liu Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
description	Abstract Pathogenic fungi including Penicillium digitatum and Penicillium italicum are the main destructive pathogens in the citrus industry, causing great losses during postharvest process. To our knowledge, only one mycovirus from P. digitatum has been reported, and the prevalence of such mycoviruses against citrus postharvest pathogenic fungi and their genotyping were still under investigation. In the present study, we showed that 39 of 152 Penicillium isolates from main citrus-growing areas in China were infected with various mycoviruses belonging to polymycoviruses, Narna-like viruses, and families Totiviridae, Partitivirdae and Chrysoviridae. The next generation sequencing (NGS) towards virus genome library and the following molecular analysis revealed two novel mycoviruses Penicillium digitatum polymycovirus 1 (PdPmV1) and Penicillium digitatum Narna-like virus 1 (PdNLV1), coexisting in P. digitatum strain HS-RH2. The fungicide-resistant P. digitatum strains HS-F6 and HS-E9 coinfected by PdPmV1 and PdNLV1 exhibited obvious reduction in triazole drug prochloraz resistance by mycelial growth analysis on both PDA plates and citrus fruit epidermis with given prochloraz concentration. This report at the first time characterized two novel mycoviruses from P. digitatum and revealed the mycovirus-induced reduction of fungicide resistance.
format	article
author	Yuhui Niu Yongze Yuan Jiali Mao Zhu Yang Qianwen Cao Tingfu Zhang Shengqiang Wang Deli Liu
author_facet	Yuhui Niu Yongze Yuan Jiali Mao Zhu Yang Qianwen Cao Tingfu Zhang Shengqiang Wang Deli Liu
author_sort	Yuhui Niu
title	Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
title_short	Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
title_full	Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
title_fullStr	Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
title_full_unstemmed	Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis
title_sort	characterization of two novel mycoviruses from penicillium digitatum and the related fungicide resistance analysis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d7977fb1596b41f68eb1c26e553c9251
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Characterization of two novel mycoviruses from Penicillium digitatum and the related fungicide resistance analysis

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