The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat

Sheyda Akhshabi,1 Esmaeil Biazar,2 Vivek Singh,3 Saeed Heidari Keshel,4 Nagaraja Geetha1 1Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India; 2Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran; 3Pro...

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Autores principales:	Akhshabi S, Biazar E, Singh V, Keshel SH, Geetha N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Collagen Chondroitin sulfate (CS) Extracellular matrix (ECM) Cross linker Electrospinning Nanofiber Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d799ab185b2c4f408e787999fffe82f5
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spelling	oai:doaj.org-article:d799ab185b2c4f408e787999fffe82f52021-12-02T07:36:46ZThe effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat1178-2013https://doaj.org/article/d799ab185b2c4f408e787999fffe82f52018-07-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-the-carbodiimide-cross-linker-on-the-structural-and-bioc-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sheyda Akhshabi,1 Esmaeil Biazar,2 Vivek Singh,3 Saeed Heidari Keshel,4 Nagaraja Geetha1 1Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India; 2Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran; 3Prof Brien Holden Eye Research Center, Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, Telangana, India; 4Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Background: Collagen and chondroitin sulfate (CS) are an essential component of the natural extracellular matrix (ECM) of most tissues. They provide the mechanical stability to cone the compressive forces in ECM. In tissue engineering, electrospun nanofibrous scaffolds prepared by electrospinning technique have emerged as a suitable candidate to imitate natural ECM functions. Cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/N-hydroxy succinimide can overcome the weak mechanical integrity of the engineered scaffolds in addition to the increased degradation stability under physiological conditions. Materials and methods: This study has synthesized nanofibrous collagen–CS scaffolds by using the electrospinning method. Results: The results have shown that incorporation of CS in higher concentration, along with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/N-hydroxy succinimide, enhanced mechanical stability. Scaffolds showed more resistance to collagenase digestion. Fabricated scaffolds showed biocompatibility in corneal epithelial cell attachment. Conclusion: These results demonstrate that cross-linked electrospun CO–CS mats exhibited a uniform nanofibrous and porous structure, especially for lower concentration of the cross-linker and may be utilized as an alternative effective substrate in tissue engineering. Keywords: collagen, chondroitin sulfate, CS, extracellular matrix, ECM, cross linker, electrospinning, nanofiberAkhshabi SBiazar ESingh VKeshel SHGeetha NDove Medical PressarticleCollagenChondroitin sulfate (CS)Extracellular matrix (ECM)Cross linkerElectrospinningNanofiberMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 4405-4416 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Collagen Chondroitin sulfate (CS) Extracellular matrix (ECM) Cross linker Electrospinning Nanofiber Medicine (General) R5-920
spellingShingle	Collagen Chondroitin sulfate (CS) Extracellular matrix (ECM) Cross linker Electrospinning Nanofiber Medicine (General) R5-920 Akhshabi S Biazar E Singh V Keshel SH Geetha N The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
description	Sheyda Akhshabi,1 Esmaeil Biazar,2 Vivek Singh,3 Saeed Heidari Keshel,4 Nagaraja Geetha1 1Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka, India; 2Department of Biomaterials Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran; 3Prof Brien Holden Eye Research Center, Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, L. V. Prasad Eye Institute, Hyderabad, Telangana, India; 4Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Background: Collagen and chondroitin sulfate (CS) are an essential component of the natural extracellular matrix (ECM) of most tissues. They provide the mechanical stability to cone the compressive forces in ECM. In tissue engineering, electrospun nanofibrous scaffolds prepared by electrospinning technique have emerged as a suitable candidate to imitate natural ECM functions. Cross-linking with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/N-hydroxy succinimide can overcome the weak mechanical integrity of the engineered scaffolds in addition to the increased degradation stability under physiological conditions. Materials and methods: This study has synthesized nanofibrous collagen–CS scaffolds by using the electrospinning method. Results: The results have shown that incorporation of CS in higher concentration, along with 1-ethyl-3-(3-dimethyl-aminopropyl)-1-carbodiimide hydrochloride/N-hydroxy succinimide, enhanced mechanical stability. Scaffolds showed more resistance to collagenase digestion. Fabricated scaffolds showed biocompatibility in corneal epithelial cell attachment. Conclusion: These results demonstrate that cross-linked electrospun CO–CS mats exhibited a uniform nanofibrous and porous structure, especially for lower concentration of the cross-linker and may be utilized as an alternative effective substrate in tissue engineering. Keywords: collagen, chondroitin sulfate, CS, extracellular matrix, ECM, cross linker, electrospinning, nanofiber
format	article
author	Akhshabi S Biazar E Singh V Keshel SH Geetha N
author_facet	Akhshabi S Biazar E Singh V Keshel SH Geetha N
author_sort	Akhshabi S
title	The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
title_short	The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
title_full	The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
title_fullStr	The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
title_full_unstemmed	The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
title_sort	effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/d799ab185b2c4f408e787999fffe82f5
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The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat

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