Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging

Ultraviolet photoacoustic microscopy (UV-PAM) has been investigated to provide label-free and registration-free volumetric histological images for whole organs, offering new insights into complex biological organs. However, because of the high UV absorption of lipids and pigments in tissue, UV-PAM s...

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Autores principales:	Xiufeng Li, Jack C.K. Kot, Victor T.C. Tsang, Claudia T.K. Lo, Bingxin Huang, Ye Tian, Ivy H.M. Wong, Helen H.Y. Cheung, Lei Kang, Atta C.Y. Chang, Terence T.W. Wong
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Photoacoustic microscopy Label-free imaging Tissue clearing Volumetric imaging Physics QC1-999 Acoustics. Sound QC221-246 Optics. Light QC350-467
Acceso en línea:	https://doaj.org/article/d79ccb8bea1d4a60b0252c66dababab3
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spelling	oai:doaj.org-article:d79ccb8bea1d4a60b0252c66dababab32021-11-06T04:28:28ZUltraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging2213-597910.1016/j.pacs.2021.100313https://doaj.org/article/d79ccb8bea1d4a60b0252c66dababab32022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2213597921000720https://doaj.org/toc/2213-5979Ultraviolet photoacoustic microscopy (UV-PAM) has been investigated to provide label-free and registration-free volumetric histological images for whole organs, offering new insights into complex biological organs. However, because of the high UV absorption of lipids and pigments in tissue, UV-PAM suffers from low image contrast and shallow image depth, hindering its capability for revealing various microstructures in organs. To improve the UV-PAM imaging contrast and imaging depth, here we propose to implement a state-of-the-art optical clearing technique, CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis), to wash out the lipids and pigments from tissues. Our results show that the UV-PAM imaging contrast and quality can be significantly improved after tissue clearing. With the cleared tissue, multilayers of cell nuclei can also be extracted from time-resolved PA signals. Tissue clearing-enhanced UV-PAM can provide fine details for organ imaging.Xiufeng LiJack C.K. KotVictor T.C. TsangClaudia T.K. LoBingxin HuangYe TianIvy H.M. WongHelen H.Y. CheungLei KangAtta C.Y. ChangTerence T.W. WongElsevierarticlePhotoacoustic microscopyLabel-free imagingTissue clearingVolumetric imagingPhysicsQC1-999Acoustics. SoundQC221-246Optics. LightQC350-467ENPhotoacoustics, Vol 25, Iss , Pp 100313- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Photoacoustic microscopy Label-free imaging Tissue clearing Volumetric imaging Physics QC1-999 Acoustics. Sound QC221-246 Optics. Light QC350-467
spellingShingle	Photoacoustic microscopy Label-free imaging Tissue clearing Volumetric imaging Physics QC1-999 Acoustics. Sound QC221-246 Optics. Light QC350-467 Xiufeng Li Jack C.K. Kot Victor T.C. Tsang Claudia T.K. Lo Bingxin Huang Ye Tian Ivy H.M. Wong Helen H.Y. Cheung Lei Kang Atta C.Y. Chang Terence T.W. Wong Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
description	Ultraviolet photoacoustic microscopy (UV-PAM) has been investigated to provide label-free and registration-free volumetric histological images for whole organs, offering new insights into complex biological organs. However, because of the high UV absorption of lipids and pigments in tissue, UV-PAM suffers from low image contrast and shallow image depth, hindering its capability for revealing various microstructures in organs. To improve the UV-PAM imaging contrast and imaging depth, here we propose to implement a state-of-the-art optical clearing technique, CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis), to wash out the lipids and pigments from tissues. Our results show that the UV-PAM imaging contrast and quality can be significantly improved after tissue clearing. With the cleared tissue, multilayers of cell nuclei can also be extracted from time-resolved PA signals. Tissue clearing-enhanced UV-PAM can provide fine details for organ imaging.
format	article
author	Xiufeng Li Jack C.K. Kot Victor T.C. Tsang Claudia T.K. Lo Bingxin Huang Ye Tian Ivy H.M. Wong Helen H.Y. Cheung Lei Kang Atta C.Y. Chang Terence T.W. Wong
author_facet	Xiufeng Li Jack C.K. Kot Victor T.C. Tsang Claudia T.K. Lo Bingxin Huang Ye Tian Ivy H.M. Wong Helen H.Y. Cheung Lei Kang Atta C.Y. Chang Terence T.W. Wong
author_sort	Xiufeng Li
title	Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
title_short	Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
title_full	Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
title_fullStr	Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
title_full_unstemmed	Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
title_sort	ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/d79ccb8bea1d4a60b0252c66dababab3
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_version_	1718443947873796096

Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging

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