Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.

Bacteria exist, in most environments, as complex, organised communities of sessile cells embedded within a matrix of self-produced, hydrated extracellular polymeric substances known as biofilms. Bacterial biofilms represent a ubiquitous and predominant cause of both chronic infections and infections...

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Autores principales:	Mahmoud Y Alkawareek, Qais Th Algwari, Garry Laverty, Sean P Gorman, William G Graham, Deborah O'Connell, Brendan F Gilmore
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7b3530dd8324767abdeb1729feaf3d8
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spelling	oai:doaj.org-article:d7b3530dd8324767abdeb1729feaf3d82021-11-18T07:06:50ZEradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.1932-620310.1371/journal.pone.0044289https://doaj.org/article/d7b3530dd8324767abdeb1729feaf3d82012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952948/?tool=EBIhttps://doaj.org/toc/1932-6203Bacteria exist, in most environments, as complex, organised communities of sessile cells embedded within a matrix of self-produced, hydrated extracellular polymeric substances known as biofilms. Bacterial biofilms represent a ubiquitous and predominant cause of both chronic infections and infections associated with the use of indwelling medical devices such as catheters and prostheses. Such infections typically exhibit significantly enhanced tolerance to antimicrobial, biocidal and immunological challenge. This renders them difficult, sometimes impossible, to treat using conventional chemotherapeutic agents. Effective alternative approaches for prevention and eradication of biofilm associated chronic and device-associated infections are therefore urgently required. Atmospheric pressure non-thermal plasmas are gaining increasing attention as a potential approach for the eradication and control of bacterial infection and contamination. To date, however, the majority of studies have been conducted with reference to planktonic bacteria and rather less attention has been directed towards bacteria in the biofilm mode of growth. In this study, the activity of a kilohertz-driven atmospheric pressure non-thermal plasma jet, operated in a helium oxygen mixture, against Pseudomonas aeruginosa in vitro biofilms was evaluated. Pseudomonas aeruginosa biofilms exhibit marked susceptibility to exposure of the plasma jet effluent, following even relatively short (≈ 10's s) exposure times. Manipulation of plasma operating conditions, for example, plasma operating frequency, had a significant effect on the bacterial inactivation rate. Survival curves exhibit a rapid decline in the number of surviving cells in the first 60 seconds followed by slower rate of cell number reduction. Excellent anti-biofilm activity of the plasma jet was also demonstrated by both confocal scanning laser microscopy and metabolism of the tetrazolium salt, XTT, a measure of bactericidal activity.Mahmoud Y AlkawareekQais Th AlgwariGarry LavertySean P GormanWilliam G GrahamDeborah O'ConnellBrendan F GilmorePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e44289 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mahmoud Y Alkawareek Qais Th Algwari Garry Laverty Sean P Gorman William G Graham Deborah O'Connell Brendan F Gilmore Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
description	Bacteria exist, in most environments, as complex, organised communities of sessile cells embedded within a matrix of self-produced, hydrated extracellular polymeric substances known as biofilms. Bacterial biofilms represent a ubiquitous and predominant cause of both chronic infections and infections associated with the use of indwelling medical devices such as catheters and prostheses. Such infections typically exhibit significantly enhanced tolerance to antimicrobial, biocidal and immunological challenge. This renders them difficult, sometimes impossible, to treat using conventional chemotherapeutic agents. Effective alternative approaches for prevention and eradication of biofilm associated chronic and device-associated infections are therefore urgently required. Atmospheric pressure non-thermal plasmas are gaining increasing attention as a potential approach for the eradication and control of bacterial infection and contamination. To date, however, the majority of studies have been conducted with reference to planktonic bacteria and rather less attention has been directed towards bacteria in the biofilm mode of growth. In this study, the activity of a kilohertz-driven atmospheric pressure non-thermal plasma jet, operated in a helium oxygen mixture, against Pseudomonas aeruginosa in vitro biofilms was evaluated. Pseudomonas aeruginosa biofilms exhibit marked susceptibility to exposure of the plasma jet effluent, following even relatively short (≈ 10's s) exposure times. Manipulation of plasma operating conditions, for example, plasma operating frequency, had a significant effect on the bacterial inactivation rate. Survival curves exhibit a rapid decline in the number of surviving cells in the first 60 seconds followed by slower rate of cell number reduction. Excellent anti-biofilm activity of the plasma jet was also demonstrated by both confocal scanning laser microscopy and metabolism of the tetrazolium salt, XTT, a measure of bactericidal activity.
format	article
author	Mahmoud Y Alkawareek Qais Th Algwari Garry Laverty Sean P Gorman William G Graham Deborah O'Connell Brendan F Gilmore
author_facet	Mahmoud Y Alkawareek Qais Th Algwari Garry Laverty Sean P Gorman William G Graham Deborah O'Connell Brendan F Gilmore
author_sort	Mahmoud Y Alkawareek
title	Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
title_short	Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
title_full	Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
title_fullStr	Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
title_full_unstemmed	Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
title_sort	eradication of pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d7b3530dd8324767abdeb1729feaf3d8
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Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma.

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