Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan

Abstract Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Cats have been reported to be infected with Leptospira spp. and shed the bacteria in the urine. However, the importance of cats as an infection source for humans remains unclear. In this study, Leptospira infection in cats in...

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Autores principales:	Tetsuya Kakita, Yumani Kuba, Hisako Kyan, Sho Okano, Masatomo Morita, Nobuo Koizumi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7b55d29781f431a9182de4b945f7340
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spelling	oai:doaj.org-article:d7b55d29781f431a9182de4b945f73402021-12-02T15:42:59ZMolecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan10.1038/s41598-021-89872-32045-2322https://doaj.org/article/d7b55d29781f431a9182de4b945f73402021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89872-3https://doaj.org/toc/2045-2322Abstract Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Cats have been reported to be infected with Leptospira spp. and shed the bacteria in the urine. However, the importance of cats as an infection source for humans remains unclear. In this study, Leptospira infection in cats in Okinawa Prefecture, Japan, where leptospirosis is endemic, was investigated by leptospiral antibody and DNA detection using microscopic agglutination test and nested PCR, respectively. Moreover, multilocus sequence typing (MLST) and whole genome sequencing (WGS) were conducted on the Leptospira borgpetersenii serogroup Javanica isolated from cats, black rats, a mongoose, and humans. Anti-Leptospira antibodies were detected in 16.6% (40/241) of the cats tested, and the predominant reactive serogroup was Javanica. The leptospiral flaB gene was detected in 7.1% (3/42) of cat urine samples, and their sequences were identical and identified as L. borgpetersenii. MLST and WGS revealed the genetic relatedness of L. borgpetersenii serogroup Javanica isolates. This study indicated that most seropositive cats had antibodies against the serogroup Javanica and that cats excreted L. borgpetersenii in the urine after infection. Further, genetic relatedness between cat and human isolates suggests that cats may be a maintenance host for L. borgpetersenii serogroup Javanica and a source for human infection.Tetsuya KakitaYumani KubaHisako KyanSho OkanoMasatomo MoritaNobuo KoizumiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tetsuya Kakita Yumani Kuba Hisako Kyan Sho Okano Masatomo Morita Nobuo Koizumi Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
description	Abstract Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. Cats have been reported to be infected with Leptospira spp. and shed the bacteria in the urine. However, the importance of cats as an infection source for humans remains unclear. In this study, Leptospira infection in cats in Okinawa Prefecture, Japan, where leptospirosis is endemic, was investigated by leptospiral antibody and DNA detection using microscopic agglutination test and nested PCR, respectively. Moreover, multilocus sequence typing (MLST) and whole genome sequencing (WGS) were conducted on the Leptospira borgpetersenii serogroup Javanica isolated from cats, black rats, a mongoose, and humans. Anti-Leptospira antibodies were detected in 16.6% (40/241) of the cats tested, and the predominant reactive serogroup was Javanica. The leptospiral flaB gene was detected in 7.1% (3/42) of cat urine samples, and their sequences were identical and identified as L. borgpetersenii. MLST and WGS revealed the genetic relatedness of L. borgpetersenii serogroup Javanica isolates. This study indicated that most seropositive cats had antibodies against the serogroup Javanica and that cats excreted L. borgpetersenii in the urine after infection. Further, genetic relatedness between cat and human isolates suggests that cats may be a maintenance host for L. borgpetersenii serogroup Javanica and a source for human infection.
format	article
author	Tetsuya Kakita Yumani Kuba Hisako Kyan Sho Okano Masatomo Morita Nobuo Koizumi
author_facet	Tetsuya Kakita Yumani Kuba Hisako Kyan Sho Okano Masatomo Morita Nobuo Koizumi
author_sort	Tetsuya Kakita
title	Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
title_short	Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
title_full	Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
title_fullStr	Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
title_full_unstemmed	Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan
title_sort	molecular and serological epidemiology of leptospira infection in cats in okinawa island, japan
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7b55d29781f431a9182de4b945f7340
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Molecular and serological epidemiology of Leptospira infection in cats in Okinawa Island, Japan

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