Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2

Systemic symptoms have often been observed in patients with coronavirus disease 2019 (COVID-19) in addition to pneumonia, however, the details are still unclear due to the lack of an appropriate animal model. In this study, we investigated and compared blood coagulation abnormalities and tissue dama...

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Autores principales: Marumi Ohno, Michihito Sasaki, Yasuko Orba, Toshiki Sekiya, Md. Abdul Masum, Osamu Ichii, Tatsuya Sawamura, Akemi Kakino, Yasuhiko Suzuki, Hiroshi Kida, Hirofumi Sawa, Masashi Shingai
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Publicado: MDPI AG 2021
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Acceso en línea:https://doaj.org/article/d7b66db7aa2a4990a890ddf699e49697
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spelling oai:doaj.org-article:d7b66db7aa2a4990a890ddf699e496972021-11-25T19:12:37ZAbnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 210.3390/v131121371999-4915https://doaj.org/article/d7b66db7aa2a4990a890ddf699e496972021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2137https://doaj.org/toc/1999-4915Systemic symptoms have often been observed in patients with coronavirus disease 2019 (COVID-19) in addition to pneumonia, however, the details are still unclear due to the lack of an appropriate animal model. In this study, we investigated and compared blood coagulation abnormalities and tissue damage between male Syrian hamsters of 9 (young) and over 36 (aged) weeks old after intranasal infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite similar levels of viral replication and inflammatory responses in the lungs of both age groups, aged but not young hamsters showed significant prolongation of prothrombin time and prominent acute kidney damage. Moreover, aged hamsters demonstrated increased intravascular coagulation time-dependently in the lungs, suggesting that consumption of coagulation factors causes prothrombin time prolongation. Furthermore, proximal urinary tract damage and mesangial matrix expansion were observed in the kidneys of the aged hamsters at early and later disease stages, respectively. Given that the severity and mortality of COVID-19 are higher in elderly human patients, the effect of aging on pathogenesis needs to be understood and should be considered for the selection of animal models. We, thus, propose that the aged hamster is a good small animal model for COVID-19 research.Marumi OhnoMichihito SasakiYasuko OrbaToshiki SekiyaMd. Abdul MasumOsamu IchiiTatsuya SawamuraAkemi KakinoYasuhiko SuzukiHiroshi KidaHirofumi SawaMasashi ShingaiMDPI AGarticleCOVID-19animal modelaged Syrian hamsterMicrobiologyQR1-502ENViruses, Vol 13, Iss 2137, p 2137 (2021)
institution DOAJ
collection DOAJ
language EN
topic COVID-19
animal model
aged Syrian hamster
Microbiology
QR1-502
spellingShingle COVID-19
animal model
aged Syrian hamster
Microbiology
QR1-502
Marumi Ohno
Michihito Sasaki
Yasuko Orba
Toshiki Sekiya
Md. Abdul Masum
Osamu Ichii
Tatsuya Sawamura
Akemi Kakino
Yasuhiko Suzuki
Hiroshi Kida
Hirofumi Sawa
Masashi Shingai
Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
description Systemic symptoms have often been observed in patients with coronavirus disease 2019 (COVID-19) in addition to pneumonia, however, the details are still unclear due to the lack of an appropriate animal model. In this study, we investigated and compared blood coagulation abnormalities and tissue damage between male Syrian hamsters of 9 (young) and over 36 (aged) weeks old after intranasal infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Despite similar levels of viral replication and inflammatory responses in the lungs of both age groups, aged but not young hamsters showed significant prolongation of prothrombin time and prominent acute kidney damage. Moreover, aged hamsters demonstrated increased intravascular coagulation time-dependently in the lungs, suggesting that consumption of coagulation factors causes prothrombin time prolongation. Furthermore, proximal urinary tract damage and mesangial matrix expansion were observed in the kidneys of the aged hamsters at early and later disease stages, respectively. Given that the severity and mortality of COVID-19 are higher in elderly human patients, the effect of aging on pathogenesis needs to be understood and should be considered for the selection of animal models. We, thus, propose that the aged hamster is a good small animal model for COVID-19 research.
format article
author Marumi Ohno
Michihito Sasaki
Yasuko Orba
Toshiki Sekiya
Md. Abdul Masum
Osamu Ichii
Tatsuya Sawamura
Akemi Kakino
Yasuhiko Suzuki
Hiroshi Kida
Hirofumi Sawa
Masashi Shingai
author_facet Marumi Ohno
Michihito Sasaki
Yasuko Orba
Toshiki Sekiya
Md. Abdul Masum
Osamu Ichii
Tatsuya Sawamura
Akemi Kakino
Yasuhiko Suzuki
Hiroshi Kida
Hirofumi Sawa
Masashi Shingai
author_sort Marumi Ohno
title Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
title_short Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
title_full Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
title_fullStr Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
title_full_unstemmed Abnormal Blood Coagulation and Kidney Damage in Aged Hamsters Infected with Severe Acute Respiratory Syndrome Coronavirus 2
title_sort abnormal blood coagulation and kidney damage in aged hamsters infected with severe acute respiratory syndrome coronavirus 2
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/d7b66db7aa2a4990a890ddf699e49697
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