Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications

ABSTRACT Engineering microbial systems allows the generation of new technologies having significant impact in the biotechnological industry and on human health. In the past few years, several synthetic biology approaches have been implemented in bacteria to allow precise engineering of novel regulat...

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Autores principales:	Cauã Antunes Westmann, María-Eugenia Guazzaroni, Rafael Silva-Rocha
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	combinatorial gene regulation CRISPR/Cas9 circuit engineering regulatory elements regulatory network synthetic biology Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d7b87afd93074a77bba54c4013b63ba8
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spelling	oai:doaj.org-article:d7b87afd93074a77bba54c4013b63ba82021-12-02T18:44:39ZEngineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications10.1128/mSystems.00151-172379-5077https://doaj.org/article/d7b87afd93074a77bba54c4013b63ba82018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00151-17https://doaj.org/toc/2379-5077ABSTRACT Engineering microbial systems allows the generation of new technologies having significant impact in the biotechnological industry and on human health. In the past few years, several synthetic biology approaches have been implemented in bacteria to allow precise engineering of novel regulatory circuits for several applications. The advent of high-throughput technologies and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-based DNA editing techniques have been pivotal in this process. Yet, despite the tremendous advances experienced recently, there are still a number of bottlenecks that need to be overcome in order to generate high-performance redesigned living machines, and the use of novel computer-aided approaches would be essential for this task. In this perspective, we discuss some of the main advances in the field of microbial engineering and the new technologies and approaches that should allow the construction of on demand synthetic microbial factories through the redesign of regulatory complexity.Cauã Antunes WestmannMaría-Eugenia GuazzaroniRafael Silva-RochaAmerican Society for Microbiologyarticlecombinatorial gene regulationCRISPR/Cas9circuit engineeringregulatory elementsregulatory networksynthetic biologyMicrobiologyQR1-502ENmSystems, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	combinatorial gene regulation CRISPR/Cas9 circuit engineering regulatory elements regulatory network synthetic biology Microbiology QR1-502
spellingShingle	combinatorial gene regulation CRISPR/Cas9 circuit engineering regulatory elements regulatory network synthetic biology Microbiology QR1-502 Cauã Antunes Westmann María-Eugenia Guazzaroni Rafael Silva-Rocha Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
description	ABSTRACT Engineering microbial systems allows the generation of new technologies having significant impact in the biotechnological industry and on human health. In the past few years, several synthetic biology approaches have been implemented in bacteria to allow precise engineering of novel regulatory circuits for several applications. The advent of high-throughput technologies and clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-based DNA editing techniques have been pivotal in this process. Yet, despite the tremendous advances experienced recently, there are still a number of bottlenecks that need to be overcome in order to generate high-performance redesigned living machines, and the use of novel computer-aided approaches would be essential for this task. In this perspective, we discuss some of the main advances in the field of microbial engineering and the new technologies and approaches that should allow the construction of on demand synthetic microbial factories through the redesign of regulatory complexity.
format	article
author	Cauã Antunes Westmann María-Eugenia Guazzaroni Rafael Silva-Rocha
author_facet	Cauã Antunes Westmann María-Eugenia Guazzaroni Rafael Silva-Rocha
author_sort	Cauã Antunes Westmann
title	Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
title_short	Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
title_full	Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
title_fullStr	Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
title_full_unstemmed	Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications
title_sort	engineering complexity in bacterial regulatory circuits for biotechnological applications
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/d7b87afd93074a77bba54c4013b63ba8
work_keys_str_mv	AT cauaantuneswestmann engineeringcomplexityinbacterialregulatorycircuitsforbiotechnologicalapplications AT mariaeugeniaguazzaroni engineeringcomplexityinbacterialregulatorycircuitsforbiotechnologicalapplications AT rafaelsilvarocha engineeringcomplexityinbacterialregulatorycircuitsforbiotechnologicalapplications
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Engineering Complexity in Bacterial Regulatory Circuits for Biotechnological Applications

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