Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum

Abstract. Prihatiningsih N, Arwiyanto T, Hadisutrisno B, Widada J. 2020. Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum. Biodiversitas 21: 4199-4204. Ralstonia solanacearum is one of the important wilt pathogens t...

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Autores principales:	Nur Prihatiningsih, Triwidodo Arwiyanto, Bambang Hadisutrisno, Jaka Widada
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antibacterial, bacillus, potato, ralstonia solanacearum, rhizosphere Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d7bb1281fff04a90a41b5add061d3809
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spelling	oai:doaj.org-article:d7bb1281fff04a90a41b5add061d38092021-11-22T00:41:43ZCharacterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum1412-033X2085-472210.13057/biodiv/d210934https://doaj.org/article/d7bb1281fff04a90a41b5add061d38092020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6216https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Prihatiningsih N, Arwiyanto T, Hadisutrisno B, Widada J. 2020. Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum. Biodiversitas 21: 4199-4204. Ralstonia solanacearum is one of the important wilt pathogens that significantly decreased potato yields in Indonesia. One of the strategies to control wilt disease is the use of Bacillus sp. that isolated from potato rhizosphere. Bacillus sp. is an antagonist against some phytopathogenic fungi and bacteria. The objectives of the study were to identify 5 strains of Bacillus spp. (B46, B209, B211, B298, and B315) that is capable of suppressing the growth of R. solanacearum. Experimental assays were performed to determine the growth inhibition of R. solanacearum by culturing in a two-layer medium. The identification of Bacillus spp. was performed based on physiological and biochemical characters and molecular identification. Results showed that all of Bacillus spp. are capable of suppressing the growth of R. solanacearum. B. subtilis B315 isolate was the most effective biocontrol agent in inhibiting R. solanacearum growth by a bacteriostatic mechanism. All Bacillus spp. have similarities in physiological and biochemical characters. Based on the molecular analysis, Bacillus sp. B46, B209, B211, and B298 were homologous to B. subtilis subspecies spizizeni RRLKE2, whereas Bacillus sp. B315 was homologous to B. subtilis strain WIFD5.Nur PrihatiningsihTriwidodo ArwiyantoBambang HadisutrisnoJaka WidadaMBI & UNS Soloarticleantibacterial, bacillus, potato, ralstonia solanacearum, rhizosphereBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibacterial, bacillus, potato, ralstonia solanacearum, rhizosphere Biology (General) QH301-705.5
spellingShingle	antibacterial, bacillus, potato, ralstonia solanacearum, rhizosphere Biology (General) QH301-705.5 Nur Prihatiningsih Triwidodo Arwiyanto Bambang Hadisutrisno Jaka Widada Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
description	Abstract. Prihatiningsih N, Arwiyanto T, Hadisutrisno B, Widada J. 2020. Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum. Biodiversitas 21: 4199-4204. Ralstonia solanacearum is one of the important wilt pathogens that significantly decreased potato yields in Indonesia. One of the strategies to control wilt disease is the use of Bacillus sp. that isolated from potato rhizosphere. Bacillus sp. is an antagonist against some phytopathogenic fungi and bacteria. The objectives of the study were to identify 5 strains of Bacillus spp. (B46, B209, B211, B298, and B315) that is capable of suppressing the growth of R. solanacearum. Experimental assays were performed to determine the growth inhibition of R. solanacearum by culturing in a two-layer medium. The identification of Bacillus spp. was performed based on physiological and biochemical characters and molecular identification. Results showed that all of Bacillus spp. are capable of suppressing the growth of R. solanacearum. B. subtilis B315 isolate was the most effective biocontrol agent in inhibiting R. solanacearum growth by a bacteriostatic mechanism. All Bacillus spp. have similarities in physiological and biochemical characters. Based on the molecular analysis, Bacillus sp. B46, B209, B211, and B298 were homologous to B. subtilis subspecies spizizeni RRLKE2, whereas Bacillus sp. B315 was homologous to B. subtilis strain WIFD5.
format	article
author	Nur Prihatiningsih Triwidodo Arwiyanto Bambang Hadisutrisno Jaka Widada
author_facet	Nur Prihatiningsih Triwidodo Arwiyanto Bambang Hadisutrisno Jaka Widada
author_sort	Nur Prihatiningsih
title	Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
title_short	Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
title_full	Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
title_fullStr	Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
title_full_unstemmed	Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum
title_sort	characterization of bacillus spp. from the rhizosphere of potato granola varieties as an antibacterial against ralstonia solanacearum
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d7bb1281fff04a90a41b5add061d3809
work_keys_str_mv	AT nurprihatiningsih characterizationofbacillussppfromtherhizosphereofpotatogranolavarietiesasanantibacterialagainstralstoniasolanacearum AT triwidodoarwiyanto characterizationofbacillussppfromtherhizosphereofpotatogranolavarietiesasanantibacterialagainstralstoniasolanacearum AT bambanghadisutrisno characterizationofbacillussppfromtherhizosphereofpotatogranolavarietiesasanantibacterialagainstralstoniasolanacearum AT jakawidada characterizationofbacillussppfromtherhizosphereofpotatogranolavarietiesasanantibacterialagainstralstoniasolanacearum
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Characterization of Bacillus spp. from the rhizosphere of potato Granola varieties as an antibacterial against Ralstonia solanacearum

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