Quantum dots modulate intracellular Ca2+ level in lung epithelial cells

Huijuan Yin,1 Jacopo M Fontana,1 Johan Solandt,1,2 Johnny Israelsson Jussi,1 Hao Xu,1 Hjalmar Brismar,1 Ying Fu1 1Section of Cellular Biophysics, Department of Applied Physics, Royal Institute of Technology (KTH), Science for Life Laboratory, Solna, 2AstraZeneca R&D, Mölndal, Swede...

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Autores principales:	Yin HJ, Fontana JM, Solandt J, Jussi JI, Xu H, Brismar H, Fu Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Calu-3 epithelial layer quantum dot intracellular Ca2+ concentration [Ca2+]i transepithelial electrical resistance cell movement Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/d7bde0eee03548a18a09c61eb24fb607
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spelling	oai:doaj.org-article:d7bde0eee03548a18a09c61eb24fb6072021-12-02T01:01:50ZQuantum dots modulate intracellular Ca2+ level in lung epithelial cells1178-2013https://doaj.org/article/d7bde0eee03548a18a09c61eb24fb6072017-04-01T00:00:00Zhttps://www.dovepress.com/quantum-dots-modulate-intracellular-ca2-level-in-lung-epithelial-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Huijuan Yin,1 Jacopo M Fontana,1 Johan Solandt,1,2 Johnny Israelsson Jussi,1 Hao Xu,1 Hjalmar Brismar,1 Ying Fu1 1Section of Cellular Biophysics, Department of Applied Physics, Royal Institute of Technology (KTH), Science for Life Laboratory, Solna, 2AstraZeneca R&D, Mölndal, Sweden Abstract: While adverse effects of nanoparticles on lung health have previously been proposed, few studies have addressed the direct effects of nanoparticle exposure on the airway epithelium. In this work, we examine the response of the pulmonary airway to nanoparticles by measuring intracellular Ca2+ concentration ([Ca2+]i) in the Calu-3 epithelial layer stimulated by 3-mercaptopropionic-acid (3MPA) coated CdSe-CdS/ZnS core-multishell quantum dots (QDs). Simultaneous transient transepithelial electrical resistance (TEER) decrease and global [Ca2+]i increase in Calu-3 epithelial layer, accompanied by cell displacements, contraction, and expansion, were observed under QD deposition. This suggests that a QD-induced global [Ca2+]i increase in the Calu-3 epithelial layer caused the transient TEER decrease. The [Ca2+]i increase was marked and rapid in the apical region, while [Ca2+]i decreased in the basolateral region of the epithelial layer. TEER transient response and extracellular Ca2+ entry induced by QD deposition were completely inhibited in cells treated with stretched-activated (SA) inhibitor GdCl3 and store-operated calcium entry (SOCE) inhibitor BTP2 and in cells immersed in Ca2+-free medium. The voltage-gated calcium channel (VGCC) inhibitor nifedipine decreased, stabilized, and suppressed the TEER response, but did not affect the [Ca2+]i increase, due to QD deposition. This demonstrates that the Ca2+ influx activated by QDs’ mechanical stretch occurs through activation of both SA and SOCE channels. QD-induced [Ca2+]i increase occurred in the Calu-3 epithelial layer after culturing for 15 days, while significant TEER drop only occurred after 23 days. This work provides a new perspective from which to study direct interactions between airway epithelium and nanoparticles and may help to reveal the pathologies of pulmonary disease. Keywords: Calu-3 epithelial layer, quantum dot, intracellular Ca2+ concentration [Ca2+]i, transepithelial electrical resistance, cell movementYin HJFontana JMSolandt JJussi JIXu HBrismar HFu YDove Medical PressarticleCalu-3 epithelial layerquantum dotintracellular Ca2+ concentration [Ca2+]itransepithelial electrical resistancecell movementMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2781-2792 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Calu-3 epithelial layer quantum dot intracellular Ca2+ concentration [Ca2+]i transepithelial electrical resistance cell movement Medicine (General) R5-920
spellingShingle	Calu-3 epithelial layer quantum dot intracellular Ca2+ concentration [Ca2+]i transepithelial electrical resistance cell movement Medicine (General) R5-920 Yin HJ Fontana JM Solandt J Jussi JI Xu H Brismar H Fu Y Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
description	Huijuan Yin,1 Jacopo M Fontana,1 Johan Solandt,1,2 Johnny Israelsson Jussi,1 Hao Xu,1 Hjalmar Brismar,1 Ying Fu1 1Section of Cellular Biophysics, Department of Applied Physics, Royal Institute of Technology (KTH), Science for Life Laboratory, Solna, 2AstraZeneca R&D, Mölndal, Sweden Abstract: While adverse effects of nanoparticles on lung health have previously been proposed, few studies have addressed the direct effects of nanoparticle exposure on the airway epithelium. In this work, we examine the response of the pulmonary airway to nanoparticles by measuring intracellular Ca2+ concentration ([Ca2+]i) in the Calu-3 epithelial layer stimulated by 3-mercaptopropionic-acid (3MPA) coated CdSe-CdS/ZnS core-multishell quantum dots (QDs). Simultaneous transient transepithelial electrical resistance (TEER) decrease and global [Ca2+]i increase in Calu-3 epithelial layer, accompanied by cell displacements, contraction, and expansion, were observed under QD deposition. This suggests that a QD-induced global [Ca2+]i increase in the Calu-3 epithelial layer caused the transient TEER decrease. The [Ca2+]i increase was marked and rapid in the apical region, while [Ca2+]i decreased in the basolateral region of the epithelial layer. TEER transient response and extracellular Ca2+ entry induced by QD deposition were completely inhibited in cells treated with stretched-activated (SA) inhibitor GdCl3 and store-operated calcium entry (SOCE) inhibitor BTP2 and in cells immersed in Ca2+-free medium. The voltage-gated calcium channel (VGCC) inhibitor nifedipine decreased, stabilized, and suppressed the TEER response, but did not affect the [Ca2+]i increase, due to QD deposition. This demonstrates that the Ca2+ influx activated by QDs’ mechanical stretch occurs through activation of both SA and SOCE channels. QD-induced [Ca2+]i increase occurred in the Calu-3 epithelial layer after culturing for 15 days, while significant TEER drop only occurred after 23 days. This work provides a new perspective from which to study direct interactions between airway epithelium and nanoparticles and may help to reveal the pathologies of pulmonary disease. Keywords: Calu-3 epithelial layer, quantum dot, intracellular Ca2+ concentration [Ca2+]i, transepithelial electrical resistance, cell movement
format	article
author	Yin HJ Fontana JM Solandt J Jussi JI Xu H Brismar H Fu Y
author_facet	Yin HJ Fontana JM Solandt J Jussi JI Xu H Brismar H Fu Y
author_sort	Yin HJ
title	Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
title_short	Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
title_full	Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
title_fullStr	Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
title_full_unstemmed	Quantum dots modulate intracellular Ca2+ level in lung epithelial cells
title_sort	quantum dots modulate intracellular ca2+ level in lung epithelial cells
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/d7bde0eee03548a18a09c61eb24fb607
work_keys_str_mv	AT yinhj quantumdotsmodulateintracellularca2levelinlungepithelialcells AT fontanajm quantumdotsmodulateintracellularca2levelinlungepithelialcells AT solandtj quantumdotsmodulateintracellularca2levelinlungepithelialcells AT jussiji quantumdotsmodulateintracellularca2levelinlungepithelialcells AT xuh quantumdotsmodulateintracellularca2levelinlungepithelialcells AT brismarh quantumdotsmodulateintracellularca2levelinlungepithelialcells AT fuy quantumdotsmodulateintracellularca2levelinlungepithelialcells
_version_	1718403347567869952

Quantum dots modulate intracellular Ca2+ level in lung epithelial cells

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