A novel mouse model of ischemic carotid artery disease.

<h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential no...

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Autores principales:	Yorito Hattori, Akihiro Kitamura, Kazuyuki Nagatsuka, Masafumi Ihara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7c0c1ea29b24b58817aca16f1fc3d4c
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spelling	oai:doaj.org-article:d7c0c1ea29b24b58817aca16f1fc3d4c2021-11-18T08:15:08ZA novel mouse model of ischemic carotid artery disease.1932-620310.1371/journal.pone.0100257https://doaj.org/article/d7c0c1ea29b24b58817aca16f1fc3d4c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24940742/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically.<h4>Methods</h4>Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes.<h4>Results</h4>The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20-30% of the baseline level before death.<h4>Conclusion</h4>The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile.Yorito HattoriAkihiro KitamuraKazuyuki NagatsukaMasafumi IharaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100257 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara A novel mouse model of ischemic carotid artery disease.
description	<h4>Background</h4>Carotid artery occlusive disease gradually develops over time, eventually leading to cerebral infarction and high mortality rate. Animal models replicating cerebral infarction resulting from carotid artery occlusive disease have thus been developed to test potential novel treatments, which could be subsequently administered clinically.<h4>Methods</h4>Adult C57BL/6J male mice were subjected to ameroid constrictor (AC) placement to gradually narrow the bilateral common carotid arteries. Cerebral blood flow (CBF) was measured at several time points. At 7 and 28 days post-operation, post-mortem brain samples were analyzed for ischemic changes.<h4>Results</h4>The mortality rate was 58.8% at 28 days post-operation. Surviving mice with AC showed continuous reduction of CBF by up to 70% of the baseline level at 28 days. Most of the mice (75%) showed multiple cerebral infarctions in the gray and white matter. Non-surviving mice showed critical CBF reduction below 20-30% of the baseline level before death.<h4>Conclusion</h4>The application of the AC on the bilateral common carotid arteries in mice could offer a reliable model of severe cerebrovascular insufficiency due to carotid artery occlusive disease and may thus be useful in exploring pharmacological intervention in stroke through monitoring survival rate, infarct formation, and CBF profile.
format	article
author	Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara
author_facet	Yorito Hattori Akihiro Kitamura Kazuyuki Nagatsuka Masafumi Ihara
author_sort	Yorito Hattori
title	A novel mouse model of ischemic carotid artery disease.
title_short	A novel mouse model of ischemic carotid artery disease.
title_full	A novel mouse model of ischemic carotid artery disease.
title_fullStr	A novel mouse model of ischemic carotid artery disease.
title_full_unstemmed	A novel mouse model of ischemic carotid artery disease.
title_sort	novel mouse model of ischemic carotid artery disease.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d7c0c1ea29b24b58817aca16f1fc3d4c
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A novel mouse model of ischemic carotid artery disease.

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