Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)

Salinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F2:3 mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map...

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Autores principales:	Xiaoyang Guo, Jinghan Jiang, Ying Liu, Lili Yu, Ruzhen Chang, Rongxia Guan, Lijuan Qiu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	salinity stress soybean gene mapping Glycine max Glycine soja Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d7c2fe38f025470bbd5505de00da64cb
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spelling	oai:doaj.org-article:d7c2fe38f025470bbd5505de00da64cb2021-11-18T09:19:53ZIdentification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)1664-462X10.3389/fpls.2021.791175https://doaj.org/article/d7c2fe38f025470bbd5505de00da64cb2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.791175/fullhttps://doaj.org/toc/1664-462XSalinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F2:3 mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map salt tolerance-related genes. The two populations segregated as 1 (tolerant):2 (heterozygous):1 (sensitive), indicating a Mendelian segregation model. Using simple sequence repeat (SSR) markers together with the bulked segregant analysis (BSA) mapping strategy, we mapped a salt tolerance locus on chromosome 03 in F2:3 population Zhonghuang39×NY36-87 to a 98-kb interval, in which the known gene GmSALT3 co-segregated with the salt tolerance locus. In the F2:3 population of Peking×NY36-87, the dominant salt tolerance-associated gene was detected and mapped on chromosome 18. We named this gene GmSALT18 and fine mapped it to a 241-kb region. Time course analysis and a grafting experiment confirmed that Peking accumulated more Na+ in the shoot via a root-based mechanism. These findings reveal that the tolerant wild soybean line NY36-87 contains salt tolerance-related genes GmSALT3 and GmSALT18, providing genetic material and a novel locus for breeding salt-tolerant soybean.Xiaoyang GuoJinghan JiangYing LiuLili YuRuzhen ChangRongxia GuanLijuan QiuFrontiers Media S.A.articlesalinity stresssoybeangene mappingGlycine maxGlycine sojaPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	salinity stress soybean gene mapping Glycine max Glycine soja Plant culture SB1-1110
spellingShingle	salinity stress soybean gene mapping Glycine max Glycine soja Plant culture SB1-1110 Xiaoyang Guo Jinghan Jiang Ying Liu Lili Yu Ruzhen Chang Rongxia Guan Lijuan Qiu Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
description	Salinity is an important abiotic stress factor that affects growth and yield of soybean. NY36-87 is a wild soybean germplasm with high salt tolerance. In this study, two F2:3 mapping populations derived from NY36-87 and two salt-sensitive soybean cultivars, Zhonghuang39 and Peking, were used to map salt tolerance-related genes. The two populations segregated as 1 (tolerant):2 (heterozygous):1 (sensitive), indicating a Mendelian segregation model. Using simple sequence repeat (SSR) markers together with the bulked segregant analysis (BSA) mapping strategy, we mapped a salt tolerance locus on chromosome 03 in F2:3 population Zhonghuang39×NY36-87 to a 98-kb interval, in which the known gene GmSALT3 co-segregated with the salt tolerance locus. In the F2:3 population of Peking×NY36-87, the dominant salt tolerance-associated gene was detected and mapped on chromosome 18. We named this gene GmSALT18 and fine mapped it to a 241-kb region. Time course analysis and a grafting experiment confirmed that Peking accumulated more Na+ in the shoot via a root-based mechanism. These findings reveal that the tolerant wild soybean line NY36-87 contains salt tolerance-related genes GmSALT3 and GmSALT18, providing genetic material and a novel locus for breeding salt-tolerant soybean.
format	article
author	Xiaoyang Guo Jinghan Jiang Ying Liu Lili Yu Ruzhen Chang Rongxia Guan Lijuan Qiu
author_facet	Xiaoyang Guo Jinghan Jiang Ying Liu Lili Yu Ruzhen Chang Rongxia Guan Lijuan Qiu
author_sort	Xiaoyang Guo
title	Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
title_short	Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
title_full	Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
title_fullStr	Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
title_full_unstemmed	Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)
title_sort	identification of a novel salt tolerance-related locus in wild soybean (glycine soja sieb. & zucc.)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d7c2fe38f025470bbd5505de00da64cb
work_keys_str_mv	AT xiaoyangguo identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT jinghanjiang identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT yingliu identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT liliyu identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT ruzhenchang identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT rongxiaguan identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc AT lijuanqiu identificationofanovelsalttolerancerelatedlocusinwildsoybeanglycinesojasiebzucc
_version_	1718420932102455296

Identification of a Novel Salt Tolerance-Related Locus in Wild Soybean (Glycine soja Sieb. & Zucc.)

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