A causal relation between bioluminescence and oxygen to quantify the cell niche.

Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival of transplanted cells. To date these assays are still largely qualitative and often erroneous due to the complexity and dynamics of local micro-e...

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Autores principales:	Dennis Lambrechts, Maarten Roeffaers, Karel Goossens, Johan Hofkens, Greetje Vande Velde, Tom Van de Putte, Jan Schrooten, Hans Van Oosterwyck
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7c327fefe0f4317bbb8259b6fcfea3d
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spelling	oai:doaj.org-article:d7c327fefe0f4317bbb8259b6fcfea3d2021-11-18T08:18:50ZA causal relation between bioluminescence and oxygen to quantify the cell niche.1932-620310.1371/journal.pone.0097572https://doaj.org/article/d7c327fefe0f4317bbb8259b6fcfea3d2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24840204/?tool=EBIhttps://doaj.org/toc/1932-6203Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival of transplanted cells. To date these assays are still largely qualitative and often erroneous due to the complexity and dynamics of local micro-environments (niches) in which the cells reside. Here, we report, using a combined experimental and computational approach, on oxygen that besides being a critical niche component responsible for cellular energy metabolism and cell-fate commitment, also serves a primary role in regulating bioluminescent light kinetics. We demonstrate the potential of an oxygen dependent Michaelis-Menten relation in quantifying intrinsic bioluminescence intensities by resolving cell-associated oxygen gradients from bioluminescent light that is emitted from three-dimensional (3D) cell-seeded hydrogels. Furthermore, the experimental and computational data indicate a strong causal relation of oxygen concentration with emitted bioluminescence intensities. Altogether our approach demonstrates the importance of oxygen to evolve towards quantitative bioluminescence and holds great potential for future microscale measurement of oxygen tension in an easily accessible manner.Dennis LambrechtsMaarten RoeffaersKarel GoossensJohan HofkensGreetje Vande VeldeTom Van de PutteJan SchrootenHans Van OosterwyckPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e97572 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dennis Lambrechts Maarten Roeffaers Karel Goossens Johan Hofkens Greetje Vande Velde Tom Van de Putte Jan Schrooten Hans Van Oosterwyck A causal relation between bioluminescence and oxygen to quantify the cell niche.
description	Bioluminescence imaging assays have become a widely integrated technique to quantify effectiveness of cell-based therapies by monitoring fate and survival of transplanted cells. To date these assays are still largely qualitative and often erroneous due to the complexity and dynamics of local micro-environments (niches) in which the cells reside. Here, we report, using a combined experimental and computational approach, on oxygen that besides being a critical niche component responsible for cellular energy metabolism and cell-fate commitment, also serves a primary role in regulating bioluminescent light kinetics. We demonstrate the potential of an oxygen dependent Michaelis-Menten relation in quantifying intrinsic bioluminescence intensities by resolving cell-associated oxygen gradients from bioluminescent light that is emitted from three-dimensional (3D) cell-seeded hydrogels. Furthermore, the experimental and computational data indicate a strong causal relation of oxygen concentration with emitted bioluminescence intensities. Altogether our approach demonstrates the importance of oxygen to evolve towards quantitative bioluminescence and holds great potential for future microscale measurement of oxygen tension in an easily accessible manner.
format	article
author	Dennis Lambrechts Maarten Roeffaers Karel Goossens Johan Hofkens Greetje Vande Velde Tom Van de Putte Jan Schrooten Hans Van Oosterwyck
author_facet	Dennis Lambrechts Maarten Roeffaers Karel Goossens Johan Hofkens Greetje Vande Velde Tom Van de Putte Jan Schrooten Hans Van Oosterwyck
author_sort	Dennis Lambrechts
title	A causal relation between bioluminescence and oxygen to quantify the cell niche.
title_short	A causal relation between bioluminescence and oxygen to quantify the cell niche.
title_full	A causal relation between bioluminescence and oxygen to quantify the cell niche.
title_fullStr	A causal relation between bioluminescence and oxygen to quantify the cell niche.
title_full_unstemmed	A causal relation between bioluminescence and oxygen to quantify the cell niche.
title_sort	causal relation between bioluminescence and oxygen to quantify the cell niche.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d7c327fefe0f4317bbb8259b6fcfea3d
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A causal relation between bioluminescence and oxygen to quantify the cell niche.

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