Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3

Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many...

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Autores principales:	Ping Zhou, Jiayu Peng, Minjie Zeng, Lixuan Wu, Yuxin Fan, Lihui Zeng
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Chinese narcissus VIGS Tobacco rattle virus (TRV) NtPDS NtMYB3 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d7c454da9f6d418799f653f6ae901ae5
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spelling	oai:doaj.org-article:d7c454da9f6d418799f653f6ae901ae52021-11-12T04:43:37ZVirus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB32468-014110.1016/j.hpj.2021.04.009https://doaj.org/article/d7c454da9f6d418799f653f6ae901ae52021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468014121001151https://doaj.org/toc/2468-0141Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many plant species. However, the VIGS system in Chinese narcissus had not been reported yet. In this paper, the first use of the TRV-VIGS system in Chinese narcissus is described in detail. By injecting pTRV2-GFP into narcissus leaves, we confirmed that TRV could infect narcissus and move in narcissus plants. After the inoculation of pTRV2-NtPDS by two methods, phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced. Besides, pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3; the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis, which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS. These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.Ping ZhouJiayu PengMinjie ZengLixuan WuYuxin FanLihui ZengKeAi Communications Co., Ltd.articleChinese narcissusVIGSTobacco rattle virus (TRV)NtPDSNtMYB3Plant cultureSB1-1110ENHorticultural Plant Journal, Vol 7, Iss 6, Pp 565-572 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Chinese narcissus VIGS Tobacco rattle virus (TRV) NtPDS NtMYB3 Plant culture SB1-1110
spellingShingle	Chinese narcissus VIGS Tobacco rattle virus (TRV) NtPDS NtMYB3 Plant culture SB1-1110 Ping Zhou Jiayu Peng Minjie Zeng Lixuan Wu Yuxin Fan Lihui Zeng Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
description	Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many plant species. However, the VIGS system in Chinese narcissus had not been reported yet. In this paper, the first use of the TRV-VIGS system in Chinese narcissus is described in detail. By injecting pTRV2-GFP into narcissus leaves, we confirmed that TRV could infect narcissus and move in narcissus plants. After the inoculation of pTRV2-NtPDS by two methods, phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced. Besides, pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3; the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis, which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS. These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.
format	article
author	Ping Zhou Jiayu Peng Minjie Zeng Lixuan Wu Yuxin Fan Lihui Zeng
author_facet	Ping Zhou Jiayu Peng Minjie Zeng Lixuan Wu Yuxin Fan Lihui Zeng
author_sort	Ping Zhou
title	Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
title_short	Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
title_full	Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
title_fullStr	Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
title_full_unstemmed	Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3
title_sort	virus-induced gene silencing (vigs) in chinese narcissus and its use in functional analysis of ntmyb3
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/d7c454da9f6d418799f653f6ae901ae5
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Virus-induced gene silencing (VIGS) in Chinese narcissus and its use in functional analysis of NtMYB3

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