Biochemistry of malaria parasite infected red blood cells by X-ray microscopy

Abstract Red blood cells infected by the malaria parasite Plasmodium falciparum are correlatively imaged by tomography using soft X-rays as well as by scanning hard nano-X-ray beam to obtain fluorescence maps of various elements such as S and Fe. In this way one can deduce the amount of Fe bound eit...

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Autores principales:	S. Kapishnikov, L. Leiserowitz, Y. Yang, P. Cloetens, E. Pereiro, F. Awamu Ndonglack, K. Matuschewski, J. Als-Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7c90505f5a44336927bbef5404af237
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spelling	oai:doaj.org-article:d7c90505f5a44336927bbef5404af2372021-12-02T16:06:04ZBiochemistry of malaria parasite infected red blood cells by X-ray microscopy10.1038/s41598-017-00921-22045-2322https://doaj.org/article/d7c90505f5a44336927bbef5404af2372017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00921-2https://doaj.org/toc/2045-2322Abstract Red blood cells infected by the malaria parasite Plasmodium falciparum are correlatively imaged by tomography using soft X-rays as well as by scanning hard nano-X-ray beam to obtain fluorescence maps of various elements such as S and Fe. In this way one can deduce the amount of Fe bound either in hemoglobin or in hemozoin crystals in the digestive vacuole of the malaria parasite as well as determine the hemoglobin concentrations in the cytosols of the red blood cell and of the parasite. Fluorescence map of K shows that in the parasite’s schizont stage the K concentration in the red blood cell cytosol is diminished by a factor of seven relative to a pristine red blood cell but the total amount of K in the infected red blood cell is the same as in the pristine red blood cell.S. KapishnikovL. LeiserowitzY. YangP. CloetensE. PereiroF. Awamu NdonglackK. MatuschewskiJ. Als-NielsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. Kapishnikov L. Leiserowitz Y. Yang P. Cloetens E. Pereiro F. Awamu Ndonglack K. Matuschewski J. Als-Nielsen Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
description	Abstract Red blood cells infected by the malaria parasite Plasmodium falciparum are correlatively imaged by tomography using soft X-rays as well as by scanning hard nano-X-ray beam to obtain fluorescence maps of various elements such as S and Fe. In this way one can deduce the amount of Fe bound either in hemoglobin or in hemozoin crystals in the digestive vacuole of the malaria parasite as well as determine the hemoglobin concentrations in the cytosols of the red blood cell and of the parasite. Fluorescence map of K shows that in the parasite’s schizont stage the K concentration in the red blood cell cytosol is diminished by a factor of seven relative to a pristine red blood cell but the total amount of K in the infected red blood cell is the same as in the pristine red blood cell.
format	article
author	S. Kapishnikov L. Leiserowitz Y. Yang P. Cloetens E. Pereiro F. Awamu Ndonglack K. Matuschewski J. Als-Nielsen
author_facet	S. Kapishnikov L. Leiserowitz Y. Yang P. Cloetens E. Pereiro F. Awamu Ndonglack K. Matuschewski J. Als-Nielsen
author_sort	S. Kapishnikov
title	Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
title_short	Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
title_full	Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
title_fullStr	Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
title_full_unstemmed	Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
title_sort	biochemistry of malaria parasite infected red blood cells by x-ray microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d7c90505f5a44336927bbef5404af237
work_keys_str_mv	AT skapishnikov biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT lleiserowitz biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT yyang biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT pcloetens biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT epereiro biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT fawamundonglack biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT kmatuschewski biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy AT jalsnielsen biochemistryofmalariaparasiteinfectedredbloodcellsbyxraymicroscopy
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Biochemistry of malaria parasite infected red blood cells by X-ray microscopy

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