Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis

Pioglitazone-loaded PLGA-PEG nanoparticles (NPs) were stabilized by the spray drying technique as an alternative to the treatment of ocular inflammatory disorders. Pioglitazone-NPs were developed and characterized physiochemically. Interaction studies, biopharmaceutical behavior, ex vivo corneal and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marcelle Silva-Abreu, Esther Miralles, Christina S. Kamma-Lorger, Marta Espina, María Luisa García, Ana Cristina Calpena
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	PLGA-PEG pioglitazone nanoparticles spray drying cornea synchrotron Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/d7ca8b97131d4958a765b3db5b28d90a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d7ca8b97131d4958a765b3db5b28d90a
record_format	dspace
spelling	oai:doaj.org-article:d7ca8b97131d4958a765b3db5b28d90a2021-11-25T18:40:21ZStabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis10.3390/pharmaceutics131117511999-4923https://doaj.org/article/d7ca8b97131d4958a765b3db5b28d90a2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1751https://doaj.org/toc/1999-4923Pioglitazone-loaded PLGA-PEG nanoparticles (NPs) were stabilized by the spray drying technique as an alternative to the treatment of ocular inflammatory disorders. Pioglitazone-NPs were developed and characterized physiochemically. Interaction studies, biopharmaceutical behavior, ex vivo corneal and scleral permeation, and in vivo bioavailability evaluations were conducted. Fibrillar diameter and interfibrillar corneal spacing of collagen was analyzed by synchrotron X-ray scattering techniques and stability studies at 4 °C and was carried out before and after the spray drying process. NPs showed physicochemical characteristics suitable for ocular administration. The release was sustained up to 46 h after drying; ex vivo corneal and scleral permeation profiles of pioglitazone-NPs before and after drying demonstrated higher retention and permeation through cornea than sclera. These results were correlated with an in vivo bioavailability study. Small-angle X-ray scattering (SAXS) analysis did not show a significant difference in the organization of the corneal collagen after the treatment with pioglitazone-NPs before and after the drying process, regarding the negative control. The stabilization process by Nano Spray Dryer B-90 was shown to be useful in preserving the activity of pioglitazone inside the NPs, maintaining their physicochemical characteristics, in vivo bioavailability, and non-damage to corneal collagen function after SAXS analysis was observed.Marcelle Silva-AbreuEsther MirallesChristina S. Kamma-LorgerMarta EspinaMaría Luisa GarcíaAna Cristina CalpenaMDPI AGarticlePLGA-PEGpioglitazonenanoparticlesspray dryingcorneasynchrotronPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1751, p 1751 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	PLGA-PEG pioglitazone nanoparticles spray drying cornea synchrotron Pharmacy and materia medica RS1-441
spellingShingle	PLGA-PEG pioglitazone nanoparticles spray drying cornea synchrotron Pharmacy and materia medica RS1-441 Marcelle Silva-Abreu Esther Miralles Christina S. Kamma-Lorger Marta Espina María Luisa García Ana Cristina Calpena Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
description	Pioglitazone-loaded PLGA-PEG nanoparticles (NPs) were stabilized by the spray drying technique as an alternative to the treatment of ocular inflammatory disorders. Pioglitazone-NPs were developed and characterized physiochemically. Interaction studies, biopharmaceutical behavior, ex vivo corneal and scleral permeation, and in vivo bioavailability evaluations were conducted. Fibrillar diameter and interfibrillar corneal spacing of collagen was analyzed by synchrotron X-ray scattering techniques and stability studies at 4 °C and was carried out before and after the spray drying process. NPs showed physicochemical characteristics suitable for ocular administration. The release was sustained up to 46 h after drying; ex vivo corneal and scleral permeation profiles of pioglitazone-NPs before and after drying demonstrated higher retention and permeation through cornea than sclera. These results were correlated with an in vivo bioavailability study. Small-angle X-ray scattering (SAXS) analysis did not show a significant difference in the organization of the corneal collagen after the treatment with pioglitazone-NPs before and after the drying process, regarding the negative control. The stabilization process by Nano Spray Dryer B-90 was shown to be useful in preserving the activity of pioglitazone inside the NPs, maintaining their physicochemical characteristics, in vivo bioavailability, and non-damage to corneal collagen function after SAXS analysis was observed.
format	article
author	Marcelle Silva-Abreu Esther Miralles Christina S. Kamma-Lorger Marta Espina María Luisa García Ana Cristina Calpena
author_facet	Marcelle Silva-Abreu Esther Miralles Christina S. Kamma-Lorger Marta Espina María Luisa García Ana Cristina Calpena
author_sort	Marcelle Silva-Abreu
title	Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
title_short	Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
title_full	Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
title_fullStr	Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
title_full_unstemmed	Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis
title_sort	stabilization by nano spray dryer of pioglitazone polymeric nanosystems: development, in vivo, ex vivo and synchrotron analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d7ca8b97131d4958a765b3db5b28d90a
work_keys_str_mv	AT marcellesilvaabreu stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis AT esthermiralles stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis AT christinaskammalorger stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis AT martaespina stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis AT marialuisagarcia stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis AT anacristinacalpena stabilizationbynanospraydryerofpioglitazonepolymericnanosystemsdevelopmentinvivoexvivoandsynchrotronanalysis
_version_	1718410865770758144

Stabilization by Nano Spray Dryer of Pioglitazone Polymeric Nanosystems: Development, In Vivo, Ex Vivo and Synchrotron Analysis

Ejemplares similares