Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity

Abstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We...

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Autores principales:	Yu Aruga, Chiaki Ikeda, Arisa Hanai, Sakiko Yoshimura, Momoko Kito, Satoe Miyaki, Misato Tsubokura, Yuka Yasuno, Chiaki Hayashi, Motoi Miyakoshi, Takahiro Nishino, Kimihiko Kawamura, Hiromichi Matsushita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7d25f2affa04020b008fc99a79005bb
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spelling	oai:doaj.org-article:d7d25f2affa04020b008fc99a79005bb2021-12-02T16:17:33ZConvenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity10.1038/s41598-021-94394-z2045-2322https://doaj.org/article/d7d25f2affa04020b008fc99a79005bb2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94394-zhttps://doaj.org/toc/2045-2322Abstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We therefore examined the effectiveness of an optical method for measuring Hgb concentrations in samples with chylous turbidity using Hgb-O in the reticulocyte channel equipped in XN-series analyzers (Sysmex, Kobe, Japan). Hgb-O showed excellent basic performance, including linear correlation and invariability with sodium lauryl sulfate (SLS)-Hgb detected by the colorimetric method. In the analysis of samples from healthy volunteers supplemented with fat emulsion, chylous turbidity did not affect Hgb-O but SLS-Hgb, which was falsely increased according to the dose of fat emulsion. Actually, SLS-Hgb was falsely elevated in 34 of 40 chylous turbidity 3+ samples. The remaining 6 samples were measured in hematology analyzers where Hgb-O was inconsistent with SLS-Hgb in the internal quality control records. For these samples, the correction factors calculated from the internal quality control records could contribute to providing the corrected Hgb-O value. These findings suggested that the optical method was effective and convenient for accurately evaluating Hgb concentrations in samples with extremely chylous turbidity.Yu ArugaChiaki IkedaArisa HanaiSakiko YoshimuraMomoko KitoSatoe MiyakiMisato TsubokuraYuka YasunoChiaki HayashiMotoi MiyakoshiTakahiro NishinoKimihiko KawamuraHiromichi MatsushitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
description	Abstract The chylous turbidity of blood samples is one of the causes of false-high hemoglobin (Hgb) concentration measurements by the colorimetric method, which has been widely applied in hematology analyzers. In such cases, additional manual procedures are required to correct Hgb concentrations. We therefore examined the effectiveness of an optical method for measuring Hgb concentrations in samples with chylous turbidity using Hgb-O in the reticulocyte channel equipped in XN-series analyzers (Sysmex, Kobe, Japan). Hgb-O showed excellent basic performance, including linear correlation and invariability with sodium lauryl sulfate (SLS)-Hgb detected by the colorimetric method. In the analysis of samples from healthy volunteers supplemented with fat emulsion, chylous turbidity did not affect Hgb-O but SLS-Hgb, which was falsely increased according to the dose of fat emulsion. Actually, SLS-Hgb was falsely elevated in 34 of 40 chylous turbidity 3+ samples. The remaining 6 samples were measured in hematology analyzers where Hgb-O was inconsistent with SLS-Hgb in the internal quality control records. For these samples, the correction factors calculated from the internal quality control records could contribute to providing the corrected Hgb-O value. These findings suggested that the optical method was effective and convenient for accurately evaluating Hgb concentrations in samples with extremely chylous turbidity.
format	article
author	Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita
author_facet	Yu Aruga Chiaki Ikeda Arisa Hanai Sakiko Yoshimura Momoko Kito Satoe Miyaki Misato Tsubokura Yuka Yasuno Chiaki Hayashi Motoi Miyakoshi Takahiro Nishino Kimihiko Kawamura Hiromichi Matsushita
author_sort	Yu Aruga
title	Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
title_short	Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
title_full	Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
title_fullStr	Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
title_full_unstemmed	Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
title_sort	convenience of hgb-o detected by optical method in xn-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7d25f2affa04020b008fc99a79005bb
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Convenience of Hgb-O detected by optical method in XN-series hematology analyzers in evaluating hemoglobin concentration in samples with chylous turbidity

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