Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.

<h4>Background</h4>Behavioral studies of Caenorhabditis elegans traditionally are done on the smooth surface of agar plates, but the natural habitat of C. elegans and other nematodes is the soil, a complex and structured environment. In order to investigate how worms move in such environ...

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Autores principales:	Sungsu Park, Hyejin Hwang, Seong-Won Nam, Fernando Martinez, Robert H Austin, William S Ryu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7d4d9e10b314b05a9c0af32142e37b2
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spelling	oai:doaj.org-article:d7d4d9e10b314b05a9c0af32142e37b22021-11-25T06:11:53ZEnhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.1932-620310.1371/journal.pone.0002550https://doaj.org/article/d7d4d9e10b314b05a9c0af32142e37b22008-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18575618/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Behavioral studies of Caenorhabditis elegans traditionally are done on the smooth surface of agar plates, but the natural habitat of C. elegans and other nematodes is the soil, a complex and structured environment. In order to investigate how worms move in such environments, we have developed a technique to study C. elegans locomotion in microstructures fabricated from agar.<h4>Methodology/principal findings</h4>When placed in open, liquid-filled, microfluidic chambers containing a square array of posts, we discovered that worms are capable of a novel mode of locomotion, which combines the fast gait of swimming with the more efficient movements of crawling. When the wavelength of the worms matched the periodicity of the post array, the microstructure directed the swimming and increased the speed of C. elegans ten-fold. We found that mutants defective in mechanosensation (mec-4, mec-10) or mutants with abnormal waveforms (unc-29) did not perform this enhanced locomotion and moved much more slowly than wild-type worms in the microstructure.<h4>Conclusion/significance</h4>These results show that the microstructure can be used as a behavioral screen for mechanosensory and uncoordinated mutants. It is likely that worms use mechanosensation in the movement and navigation through heterogeneous environments.Sungsu ParkHyejin HwangSeong-Won NamFernando MartinezRobert H AustinWilliam S RyuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 6, p e2550 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sungsu Park Hyejin Hwang Seong-Won Nam Fernando Martinez Robert H Austin William S Ryu Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
description	<h4>Background</h4>Behavioral studies of Caenorhabditis elegans traditionally are done on the smooth surface of agar plates, but the natural habitat of C. elegans and other nematodes is the soil, a complex and structured environment. In order to investigate how worms move in such environments, we have developed a technique to study C. elegans locomotion in microstructures fabricated from agar.<h4>Methodology/principal findings</h4>When placed in open, liquid-filled, microfluidic chambers containing a square array of posts, we discovered that worms are capable of a novel mode of locomotion, which combines the fast gait of swimming with the more efficient movements of crawling. When the wavelength of the worms matched the periodicity of the post array, the microstructure directed the swimming and increased the speed of C. elegans ten-fold. We found that mutants defective in mechanosensation (mec-4, mec-10) or mutants with abnormal waveforms (unc-29) did not perform this enhanced locomotion and moved much more slowly than wild-type worms in the microstructure.<h4>Conclusion/significance</h4>These results show that the microstructure can be used as a behavioral screen for mechanosensory and uncoordinated mutants. It is likely that worms use mechanosensation in the movement and navigation through heterogeneous environments.
format	article
author	Sungsu Park Hyejin Hwang Seong-Won Nam Fernando Martinez Robert H Austin William S Ryu
author_facet	Sungsu Park Hyejin Hwang Seong-Won Nam Fernando Martinez Robert H Austin William S Ryu
author_sort	Sungsu Park
title	Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
title_short	Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
title_full	Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
title_fullStr	Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
title_full_unstemmed	Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.
title_sort	enhanced caenorhabditis elegans locomotion in a structured microfluidic environment.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/d7d4d9e10b314b05a9c0af32142e37b2
work_keys_str_mv	AT sungsupark enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment AT hyejinhwang enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment AT seongwonnam enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment AT fernandomartinez enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment AT roberthaustin enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment AT williamsryu enhancedcaenorhabditiseleganslocomotioninastructuredmicrofluidicenvironment
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Enhanced Caenorhabditis elegans locomotion in a structured microfluidic environment.

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