Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.

The success of the Asian bivalve Limnoperna fortunei as an invader in South America is related to its high acclimation capability. It can inhabit waters with a wide range of temperatures and salinity and handle long-term periods of air exposure. We describe the transcriptome of L. fortunei aiming to...

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Autores principales:	Marcela Uliano-Silva, Juliana Alves Americo, Rodrigo Brindeiro, Francesco Dondero, Francisco Prosdocimi, Mauro de Freitas Rebelo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7d4da6a12784fb1bf27733902a38990
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spelling	oai:doaj.org-article:d7d4da6a12784fb1bf27733902a389902021-11-25T06:07:48ZGene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.1932-620310.1371/journal.pone.0102973https://doaj.org/article/d7d4da6a12784fb1bf27733902a389902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25047650/?tool=EBIhttps://doaj.org/toc/1932-6203The success of the Asian bivalve Limnoperna fortunei as an invader in South America is related to its high acclimation capability. It can inhabit waters with a wide range of temperatures and salinity and handle long-term periods of air exposure. We describe the transcriptome of L. fortunei aiming to give a first insight into the phenotypic plasticity that allows non-native taxa to become established and widespread. We sequenced 95,219 reads from five main tissues of the mussel L. fortunei using Roche's 454 and assembled them to form a set of 84,063 unigenes (contigs and singletons) representing partial or complete gene sequences. We annotated 24,816 unigenes using a BLAST sequence similarity search against a NCBI nr database. Unigenes were divided into 20 eggNOG functional categories and 292 KEGG metabolic pathways. From the total unigenes, 1,351 represented putative full-length genes of which 73.2% were functionally annotated. We described the first partial and complete gene sequences in order to start understanding bivalve invasiveness. An expansion of the hsp70 gene family, seen also in other bivalves, is present in L. fortunei and could be involved in its adaptation to extreme environments, e.g. during intertidal periods. The presence of toll-like receptors gives a first insight into an immune system that could be more complex than previously assumed and may be involved in the prevention of disease and extinction when population densities are high. Finally, the apparent lack of special adaptations to extremely low O2 levels is a target worth pursuing for the development of a molecular control approach.Marcela Uliano-SilvaJuliana Alves AmericoRodrigo BrindeiroFrancesco DonderoFrancisco ProsdocimiMauro de Freitas RebeloPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e102973 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcela Uliano-Silva Juliana Alves Americo Rodrigo Brindeiro Francesco Dondero Francisco Prosdocimi Mauro de Freitas Rebelo Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
description	The success of the Asian bivalve Limnoperna fortunei as an invader in South America is related to its high acclimation capability. It can inhabit waters with a wide range of temperatures and salinity and handle long-term periods of air exposure. We describe the transcriptome of L. fortunei aiming to give a first insight into the phenotypic plasticity that allows non-native taxa to become established and widespread. We sequenced 95,219 reads from five main tissues of the mussel L. fortunei using Roche's 454 and assembled them to form a set of 84,063 unigenes (contigs and singletons) representing partial or complete gene sequences. We annotated 24,816 unigenes using a BLAST sequence similarity search against a NCBI nr database. Unigenes were divided into 20 eggNOG functional categories and 292 KEGG metabolic pathways. From the total unigenes, 1,351 represented putative full-length genes of which 73.2% were functionally annotated. We described the first partial and complete gene sequences in order to start understanding bivalve invasiveness. An expansion of the hsp70 gene family, seen also in other bivalves, is present in L. fortunei and could be involved in its adaptation to extreme environments, e.g. during intertidal periods. The presence of toll-like receptors gives a first insight into an immune system that could be more complex than previously assumed and may be involved in the prevention of disease and extinction when population densities are high. Finally, the apparent lack of special adaptations to extremely low O2 levels is a target worth pursuing for the development of a molecular control approach.
format	article
author	Marcela Uliano-Silva Juliana Alves Americo Rodrigo Brindeiro Francesco Dondero Francisco Prosdocimi Mauro de Freitas Rebelo
author_facet	Marcela Uliano-Silva Juliana Alves Americo Rodrigo Brindeiro Francesco Dondero Francisco Prosdocimi Mauro de Freitas Rebelo
author_sort	Marcela Uliano-Silva
title	Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
title_short	Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
title_full	Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
title_fullStr	Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
title_full_unstemmed	Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.
title_sort	gene discovery through transcriptome sequencing for the invasive mussel limnoperna fortunei.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d7d4da6a12784fb1bf27733902a38990
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Gene discovery through transcriptome sequencing for the invasive mussel Limnoperna fortunei.

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