Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site

Abstract Photosystem II (PSII) reaction centre D1 protein of oxygenic phototrophs is pivotal for sustaining photosynthesis. Also, it is targeted by herbicides and herbicide-resistant weeds harbour single amino acid substitutions in D1. Conservation of D1 primary structure is seminal in the photosynt...

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Autores principales:	Amina Antonacci, Maya D. Lambreva, Andrea Margonelli, Anatoly P. Sobolev, Sandro Pastorelli, Ivo Bertalan, Udo Johanningmeier, Vladimir Sobolev, Ilan Samish, Marvin Edelman, Vesa Havurinne, Esa Tyystjärvi, Maria T. Giardi, Autar K. Mattoo, Giuseppina Rea
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Photosystem II (PSII) Metabolic Rewiring Zeaxanthin High Photon Fluence Photosynthetic Performance Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7d59f7fe000484b90acc5aa2ca7929f
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spelling	oai:doaj.org-article:d7d59f7fe000484b90acc5aa2ca7929f2021-12-02T11:40:46ZPhotosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site10.1038/s41598-018-33146-y2045-2322https://doaj.org/article/d7d59f7fe000484b90acc5aa2ca7929f2018-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-33146-yhttps://doaj.org/toc/2045-2322Abstract Photosystem II (PSII) reaction centre D1 protein of oxygenic phototrophs is pivotal for sustaining photosynthesis. Also, it is targeted by herbicides and herbicide-resistant weeds harbour single amino acid substitutions in D1. Conservation of D1 primary structure is seminal in the photosynthetic performance in many diverse species. In this study, we analysed built-in and environmentally-induced (high temperature and high photon fluency – HT/HL) phenotypes of two D1 mutants of Chlamydomonas reinhardtii with Ala250Arg (A250R) and Ser264Lys (S264K) substitutions. Both mutations differentially affected efficiency of electron transport and oxygen production. In addition, targeted metabolomics revealed that the mutants undergo specific differences in primary and secondary metabolism, namely, amino acids, organic acids, pigments, NAD, xanthophylls and carotenes. Levels of lutein, β-carotene and zeaxanthin were in sync with their corresponding gene transcripts in response to HT/HL stress treatment in the parental (IL) and A250R strains. D1 structure analysis indicated that, among other effects, remodelling of H-bond network at the QB site might underpin the observed phenotypes. Thus, the D1 protein, in addition to being pivotal for efficient photosynthesis, may have a moonlighting role in rewiring of specific metabolic pathways, possibly involving retrograde signalling.Amina AntonacciMaya D. LambrevaAndrea MargonelliAnatoly P. SobolevSandro PastorelliIvo BertalanUdo JohanningmeierVladimir SobolevIlan SamishMarvin EdelmanVesa HavurinneEsa TyystjärviMaria T. GiardiAutar K. MattooGiuseppina ReaNature PortfolioarticlePhotosystem II (PSII)Metabolic RewiringZeaxanthinHigh Photon FluencePhotosynthetic PerformanceMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Photosystem II (PSII) Metabolic Rewiring Zeaxanthin High Photon Fluence Photosynthetic Performance Medicine R Science Q
spellingShingle	Photosystem II (PSII) Metabolic Rewiring Zeaxanthin High Photon Fluence Photosynthetic Performance Medicine R Science Q Amina Antonacci Maya D. Lambreva Andrea Margonelli Anatoly P. Sobolev Sandro Pastorelli Ivo Bertalan Udo Johanningmeier Vladimir Sobolev Ilan Samish Marvin Edelman Vesa Havurinne Esa Tyystjärvi Maria T. Giardi Autar K. Mattoo Giuseppina Rea Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
description	Abstract Photosystem II (PSII) reaction centre D1 protein of oxygenic phototrophs is pivotal for sustaining photosynthesis. Also, it is targeted by herbicides and herbicide-resistant weeds harbour single amino acid substitutions in D1. Conservation of D1 primary structure is seminal in the photosynthetic performance in many diverse species. In this study, we analysed built-in and environmentally-induced (high temperature and high photon fluency – HT/HL) phenotypes of two D1 mutants of Chlamydomonas reinhardtii with Ala250Arg (A250R) and Ser264Lys (S264K) substitutions. Both mutations differentially affected efficiency of electron transport and oxygen production. In addition, targeted metabolomics revealed that the mutants undergo specific differences in primary and secondary metabolism, namely, amino acids, organic acids, pigments, NAD, xanthophylls and carotenes. Levels of lutein, β-carotene and zeaxanthin were in sync with their corresponding gene transcripts in response to HT/HL stress treatment in the parental (IL) and A250R strains. D1 structure analysis indicated that, among other effects, remodelling of H-bond network at the QB site might underpin the observed phenotypes. Thus, the D1 protein, in addition to being pivotal for efficient photosynthesis, may have a moonlighting role in rewiring of specific metabolic pathways, possibly involving retrograde signalling.
format	article
author	Amina Antonacci Maya D. Lambreva Andrea Margonelli Anatoly P. Sobolev Sandro Pastorelli Ivo Bertalan Udo Johanningmeier Vladimir Sobolev Ilan Samish Marvin Edelman Vesa Havurinne Esa Tyystjärvi Maria T. Giardi Autar K. Mattoo Giuseppina Rea
author_facet	Amina Antonacci Maya D. Lambreva Andrea Margonelli Anatoly P. Sobolev Sandro Pastorelli Ivo Bertalan Udo Johanningmeier Vladimir Sobolev Ilan Samish Marvin Edelman Vesa Havurinne Esa Tyystjärvi Maria T. Giardi Autar K. Mattoo Giuseppina Rea
author_sort	Amina Antonacci
title	Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
title_short	Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
title_full	Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
title_fullStr	Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
title_full_unstemmed	Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site
title_sort	photosystem-ii d1 protein mutants of chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of h-bond network at qb site
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/d7d59f7fe000484b90acc5aa2ca7929f
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Photosystem-II D1 protein mutants of Chlamydomonas reinhardtii in relation to metabolic rewiring and remodelling of H-bond network at QB site

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