Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery

Polymeric micelle-like nanoparticles have demonstrated effectiveness for the delivery of some poorly soluble or hydrophobic anticancer drugs. In this study, a hydrophobic moiety, deoxycholic acid (DCA) was first bonded on a polysaccharide, chitosan (CS), for the preparation of amphiphilic chitosan (...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiudong Liu, Huofei Zhou, Weiting Yu, Xin Xiong, Rumen Krastev, Xiaojun Ma
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cationic amphiphilic chitosan self-assembled nanoparticles doxorubicin drug delivery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
Acceso en línea:	https://doaj.org/article/d7dc7d719a53461d936425a5b8d3c25b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d7dc7d719a53461d936425a5b8d3c25b
record_format	dspace
spelling	oai:doaj.org-article:d7dc7d719a53461d936425a5b8d3c25b2021-11-25T18:15:39ZPreparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery10.3390/ma142270101996-1944https://doaj.org/article/d7dc7d719a53461d936425a5b8d3c25b2021-11-01T00:00:00Zhttps://www.mdpi.com/1996-1944/14/22/7010https://doaj.org/toc/1996-1944Polymeric micelle-like nanoparticles have demonstrated effectiveness for the delivery of some poorly soluble or hydrophobic anticancer drugs. In this study, a hydrophobic moiety, deoxycholic acid (DCA) was first bonded on a polysaccharide, chitosan (CS), for the preparation of amphiphilic chitosan (CS-DCA), which was further modified with a cationic glycidyltrimethylammounium chloride (GTMAC) to form a novel soluble chitosan derivative (HT-CS-DCA). The cationic amphiphilic HT-CS-DCA was easily self-assembled to micelle-like nanoparticles about 200 nm with narrow size distribution (PDI 0.08–0.18). The zeta potential of nanoparticles was in the range of 14 to 24 mV, indicating higher positive charges. Then, doxorubicin (DOX), an anticancer drug with poor solubility, was entrapped into HT-CS-DCA nanoparticles. The DOX release test was performed in PBS (pH 7.4) at 37 °C, and the results showed that there was no significant burst release in the first two hours, and the cumulative release increased steadily and slowly in the following hours. HT-CS-DCA nanoparticles loaded with DOX could easily enter into MCF-7 cells, as observed by a confocal microscope. As a result, DOX-loaded HT-CS-DCA nanoparticles demonstrated a significant inhibition activity on MCF-7 growth without obvious cellular toxicity in comparison with blank nanoparticles. Therefore, the anticancer efficacy of these cationic HT-CS-DCA nanoparticles showed great promise for the delivery of DOX in cancer therapy.Xiudong LiuHuofei ZhouWeiting YuXin XiongRumen KrastevXiaojun MaMDPI AGarticlecationic amphiphilic chitosanself-assembled nanoparticlesdoxorubicindrug deliveryTechnologyTElectrical engineering. Electronics. Nuclear engineeringTK1-9971Engineering (General). Civil engineering (General)TA1-2040MicroscopyQH201-278.5Descriptive and experimental mechanicsQC120-168.85ENMaterials, Vol 14, Iss 7010, p 7010 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cationic amphiphilic chitosan self-assembled nanoparticles doxorubicin drug delivery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85
spellingShingle	cationic amphiphilic chitosan self-assembled nanoparticles doxorubicin drug delivery Technology T Electrical engineering. Electronics. Nuclear engineering TK1-9971 Engineering (General). Civil engineering (General) TA1-2040 Microscopy QH201-278.5 Descriptive and experimental mechanics QC120-168.85 Xiudong Liu Huofei Zhou Weiting Yu Xin Xiong Rumen Krastev Xiaojun Ma Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
description	Polymeric micelle-like nanoparticles have demonstrated effectiveness for the delivery of some poorly soluble or hydrophobic anticancer drugs. In this study, a hydrophobic moiety, deoxycholic acid (DCA) was first bonded on a polysaccharide, chitosan (CS), for the preparation of amphiphilic chitosan (CS-DCA), which was further modified with a cationic glycidyltrimethylammounium chloride (GTMAC) to form a novel soluble chitosan derivative (HT-CS-DCA). The cationic amphiphilic HT-CS-DCA was easily self-assembled to micelle-like nanoparticles about 200 nm with narrow size distribution (PDI 0.08–0.18). The zeta potential of nanoparticles was in the range of 14 to 24 mV, indicating higher positive charges. Then, doxorubicin (DOX), an anticancer drug with poor solubility, was entrapped into HT-CS-DCA nanoparticles. The DOX release test was performed in PBS (pH 7.4) at 37 °C, and the results showed that there was no significant burst release in the first two hours, and the cumulative release increased steadily and slowly in the following hours. HT-CS-DCA nanoparticles loaded with DOX could easily enter into MCF-7 cells, as observed by a confocal microscope. As a result, DOX-loaded HT-CS-DCA nanoparticles demonstrated a significant inhibition activity on MCF-7 growth without obvious cellular toxicity in comparison with blank nanoparticles. Therefore, the anticancer efficacy of these cationic HT-CS-DCA nanoparticles showed great promise for the delivery of DOX in cancer therapy.
format	article
author	Xiudong Liu Huofei Zhou Weiting Yu Xin Xiong Rumen Krastev Xiaojun Ma
author_facet	Xiudong Liu Huofei Zhou Weiting Yu Xin Xiong Rumen Krastev Xiaojun Ma
author_sort	Xiudong Liu
title	Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
title_short	Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
title_full	Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
title_fullStr	Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
title_full_unstemmed	Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery
title_sort	preparation of cationic amphiphilic nanoparticles with modified chitosan derivatives for doxorubicin delivery
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d7dc7d719a53461d936425a5b8d3c25b
work_keys_str_mv	AT xiudongliu preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery AT huofeizhou preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery AT weitingyu preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery AT xinxiong preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery AT rumenkrastev preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery AT xiaojunma preparationofcationicamphiphilicnanoparticleswithmodifiedchitosanderivativesfordoxorubicindelivery
_version_	1718411468926353408

Preparation of Cationic Amphiphilic Nanoparticles with Modified Chitosan Derivatives for Doxorubicin Delivery

Ejemplares similares