Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation

The study was undertaken considering: i) that relative proportion of distinct subsets of splenic dendritic cells (DCs) is strain-specific and predictive for the susceptibility to autoimmune diseases; ii) age-related changes in endocytic, allostimulatory and polarizing capacity of splenic OX62+ DCs f...

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Autores principales:	Biljana BUFAN, Zorica STOJIĆ-VUKANIĆ, Jasmina DJIKIĆ, Duško KOSEC, Ivan PILIPOVIĆ, Mirjana NACKA-ALEKSIĆ, Nevena ARSENOVIĆ-RANIN, Gordana LEPOSAVIĆ
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	aging allostimulatory capacity splenic conventional dendritic cells strain differences th polarization Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d7dd2c7729294a62b02e761c8a22ef8d
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spelling	oai:doaj.org-article:d7dd2c7729294a62b02e761c8a22ef8d2021-11-17T21:27:50ZAging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation1820-744810.1515/acve-2015-0003https://doaj.org/article/d7dd2c7729294a62b02e761c8a22ef8d2015-03-01T00:00:00Zhttps://doi.org/10.1515/acve-2015-0003https://doaj.org/toc/1820-7448The study was undertaken considering: i) that relative proportion of distinct subsets of splenic dendritic cells (DCs) is strain-specific and predictive for the susceptibility to autoimmune diseases; ii) age-related changes in endocytic, allostimulatory and polarizing capacity of splenic OX62+ DCs from Albino Oxford rats (relatively resistant to Th1/Th17-mediated diseases) and iii) strain specificities in age-related changes of mouse DCs. To ascertain whether there are strain specificities in age-related rat DC changes, we examined the influence of aging on OX62+ DCs from Dark Agouti (DA) rats prone to Th1/Th17-mediated autoimmune diseases. The study provided additional evidence that the predominance of CD4-cells within OX62+ DCs from young adult rats correlates with their susceptibility to Th1/Th17-mediated diseases. Consistently, lipopolysaccharide (LPS)-matured DCs from 3-month-old (young) rats exhibited Th1 driving force when co-cultured with allogeneic CD4+ T cells. This most likely reflected enhanced TNF-α and iNOS expression. Comparing with young rats, OX62+ DCs from 26-month-old (aged) rats showed: i) diminished endocytic capacity; ii) impaired ability to mature in vitro upon LPS stimulation (as indicated by lower MHC II, CD86 and CD40 surface expression), which is consistent with the increase in their IL-10 production, and iii) diminished allostimulatory capacity and loss of Th1-driving capacity in the mixed lymphocyte reaction. The latter, probably, reflected greater IL-10 production by LPS-stimulated DC from aged rats, as well as lower CD40 density on their surface. Overall, our findings suggest that aging might affect DA rat capability to mount an efficient Th1 immune response, and consequently susceptibility to Th1/Th17-mediated pathology.Biljana BUFANZorica STOJIĆ-VUKANIĆJasmina DJIKIĆDuško KOSECIvan PILIPOVIĆMirjana NACKA-ALEKSIĆNevena ARSENOVIĆ-RANINGordana LEPOSAVIĆSciendoarticleagingallostimulatory capacitysplenic conventional dendritic cellsstrain differencesth polarizationVeterinary medicineSF600-1100ENActa Veterinaria, Vol 65, Iss 1, Pp 30-55 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	aging allostimulatory capacity splenic conventional dendritic cells strain differences th polarization Veterinary medicine SF600-1100
spellingShingle	aging allostimulatory capacity splenic conventional dendritic cells strain differences th polarization Veterinary medicine SF600-1100 Biljana BUFAN Zorica STOJIĆ-VUKANIĆ Jasmina DJIKIĆ Duško KOSEC Ivan PILIPOVIĆ Mirjana NACKA-ALEKSIĆ Nevena ARSENOVIĆ-RANIN Gordana LEPOSAVIĆ Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
description	The study was undertaken considering: i) that relative proportion of distinct subsets of splenic dendritic cells (DCs) is strain-specific and predictive for the susceptibility to autoimmune diseases; ii) age-related changes in endocytic, allostimulatory and polarizing capacity of splenic OX62+ DCs from Albino Oxford rats (relatively resistant to Th1/Th17-mediated diseases) and iii) strain specificities in age-related changes of mouse DCs. To ascertain whether there are strain specificities in age-related rat DC changes, we examined the influence of aging on OX62+ DCs from Dark Agouti (DA) rats prone to Th1/Th17-mediated autoimmune diseases. The study provided additional evidence that the predominance of CD4-cells within OX62+ DCs from young adult rats correlates with their susceptibility to Th1/Th17-mediated diseases. Consistently, lipopolysaccharide (LPS)-matured DCs from 3-month-old (young) rats exhibited Th1 driving force when co-cultured with allogeneic CD4+ T cells. This most likely reflected enhanced TNF-α and iNOS expression. Comparing with young rats, OX62+ DCs from 26-month-old (aged) rats showed: i) diminished endocytic capacity; ii) impaired ability to mature in vitro upon LPS stimulation (as indicated by lower MHC II, CD86 and CD40 surface expression), which is consistent with the increase in their IL-10 production, and iii) diminished allostimulatory capacity and loss of Th1-driving capacity in the mixed lymphocyte reaction. The latter, probably, reflected greater IL-10 production by LPS-stimulated DC from aged rats, as well as lower CD40 density on their surface. Overall, our findings suggest that aging might affect DA rat capability to mount an efficient Th1 immune response, and consequently susceptibility to Th1/Th17-mediated pathology.
format	article
author	Biljana BUFAN Zorica STOJIĆ-VUKANIĆ Jasmina DJIKIĆ Duško KOSEC Ivan PILIPOVIĆ Mirjana NACKA-ALEKSIĆ Nevena ARSENOVIĆ-RANIN Gordana LEPOSAVIĆ
author_facet	Biljana BUFAN Zorica STOJIĆ-VUKANIĆ Jasmina DJIKIĆ Duško KOSEC Ivan PILIPOVIĆ Mirjana NACKA-ALEKSIĆ Nevena ARSENOVIĆ-RANIN Gordana LEPOSAVIĆ
author_sort	Biljana BUFAN
title	Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
title_short	Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
title_full	Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
title_fullStr	Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
title_full_unstemmed	Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation
title_sort	aging impairs endocytic capacity of splenic dendritic cells from dark agouti rats and alters their response to tlr4 stimulation
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/d7dd2c7729294a62b02e761c8a22ef8d
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Aging Impairs Endocytic Capacity Of Splenic Dendritic Cells From Dark Agouti Rats And Alters Their Response To TLR4 Stimulation

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