Effects of PVA coated nanoparticles on human immune cells

Cindy Strehl,1,2 Timo Gaber,1–3 Lionel Maurizi,4 Martin Hahne,1,2 Roman Rauch,1,2 Paula Hoff,1–3 Thomas Häupl,1 Margarethe Hofmann-Amtenbrink,5 A Robin Poole,6 Heinrich Hofmann,4 Frank Buttgereit1–3 1Department of Rheumatology and Clinical Immunology, Charit&a...

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Autores principales: Strehl C, Gaber T, Maurizi L, Hahne M, Rauch R, Hoff P, Häupl T, Hofmann-Amtenbrink M, Poole AR, Hofmann H, Buttgereit F
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Publicado: Dove Medical Press 2015
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spelling oai:doaj.org-article:d7e32c3db6cd4d2da5e0fc6bb83b07112021-12-02T02:04:16ZEffects of PVA coated nanoparticles on human immune cells1178-2013https://doaj.org/article/d7e32c3db6cd4d2da5e0fc6bb83b07112015-05-01T00:00:00Zhttp://www.dovepress.com/effects-of-pva-coated-nanoparticles-on-human-immune-cells-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Cindy Strehl,1,2 Timo Gaber,1–3 Lionel Maurizi,4 Martin Hahne,1,2 Roman Rauch,1,2 Paula Hoff,1–3 Thomas Häupl,1 Margarethe Hofmann-Amtenbrink,5 A Robin Poole,6 Heinrich Hofmann,4 Frank Buttgereit1–3 1Department of Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Berlin, Germany; 2German Rheumatism Research Centre (DRFZ), Berlin, Germany; 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany; 4Powder Technology Laboratory, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland; 5MatSearch Consulting Hofmann, Pully-Lausanne, Switzerland; 6Department of Surgery, McGill University, Montreal, QC, Canada Abstract: Nanotechnology provides new opportunities in human medicine, mainly for diagnostic and therapeutic purposes. The autoimmune disease rheumatoid arthritis (RA) is often diagnosed after irreversible joint structural damage has occurred. There is an urgent need for a very early diagnosis of RA, which can be achieved by more sensitive imaging methods. Superparamagnetic iron oxide nanoparticles (SPION) are already used in medicine and therefore represent a promising tool for early diagnosis of RA. The focus of our work was to investigate any potentially negative effects resulting from the interactions of newly developed amino-functionalized amino-polyvinyl alcohol coated (a-PVA) SPION (a-PVA-SPION), that are used for imaging, with human immune cells. We analyzed the influence of a-PVA-SPION with regard to cell survival and cell activation in human whole blood in general, and in human monocytes and macrophages representative of professional phagocytes, using flow cytometry, multiplex suspension array, and transmission electron microscopy. We found no effect of a-PVA-SPION on the viability of human immune cells, but cytokine secretion was affected. We further demonstrated that the percentage of viable macrophages increased on exposure to a-PVA-SPION. This effect was even stronger when a-PVA-SPION were added very early in the differentiation process. Additionally, transmission electron microscopy analysis revealed that both monocytes and macrophages are able to endocytose a-PVA-SPION. Our findings demonstrate an interaction between human immune cells and a-PVA-SPION which needs to be taken into account when considering the use of a-PVA-SPION in human medicine. Keywords: nanoparticle, cell viability, cytokine, monocyte, macrophageStrehl CGaber TMaurizi LHahne MRauch RHoff PHäupl THofmann-Amtenbrink MPoole ARHofmann HButtgereit FDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3429-3445 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Strehl C
Gaber T
Maurizi L
Hahne M
Rauch R
Hoff P
Häupl T
Hofmann-Amtenbrink M
Poole AR
Hofmann H
Buttgereit F
Effects of PVA coated nanoparticles on human immune cells
description Cindy Strehl,1,2 Timo Gaber,1–3 Lionel Maurizi,4 Martin Hahne,1,2 Roman Rauch,1,2 Paula Hoff,1–3 Thomas Häupl,1 Margarethe Hofmann-Amtenbrink,5 A Robin Poole,6 Heinrich Hofmann,4 Frank Buttgereit1–3 1Department of Rheumatology and Clinical Immunology, Charité Universitätsmedizin Berlin, Berlin, Germany; 2German Rheumatism Research Centre (DRFZ), Berlin, Germany; 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany; 4Powder Technology Laboratory, Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland; 5MatSearch Consulting Hofmann, Pully-Lausanne, Switzerland; 6Department of Surgery, McGill University, Montreal, QC, Canada Abstract: Nanotechnology provides new opportunities in human medicine, mainly for diagnostic and therapeutic purposes. The autoimmune disease rheumatoid arthritis (RA) is often diagnosed after irreversible joint structural damage has occurred. There is an urgent need for a very early diagnosis of RA, which can be achieved by more sensitive imaging methods. Superparamagnetic iron oxide nanoparticles (SPION) are already used in medicine and therefore represent a promising tool for early diagnosis of RA. The focus of our work was to investigate any potentially negative effects resulting from the interactions of newly developed amino-functionalized amino-polyvinyl alcohol coated (a-PVA) SPION (a-PVA-SPION), that are used for imaging, with human immune cells. We analyzed the influence of a-PVA-SPION with regard to cell survival and cell activation in human whole blood in general, and in human monocytes and macrophages representative of professional phagocytes, using flow cytometry, multiplex suspension array, and transmission electron microscopy. We found no effect of a-PVA-SPION on the viability of human immune cells, but cytokine secretion was affected. We further demonstrated that the percentage of viable macrophages increased on exposure to a-PVA-SPION. This effect was even stronger when a-PVA-SPION were added very early in the differentiation process. Additionally, transmission electron microscopy analysis revealed that both monocytes and macrophages are able to endocytose a-PVA-SPION. Our findings demonstrate an interaction between human immune cells and a-PVA-SPION which needs to be taken into account when considering the use of a-PVA-SPION in human medicine. Keywords: nanoparticle, cell viability, cytokine, monocyte, macrophage
format article
author Strehl C
Gaber T
Maurizi L
Hahne M
Rauch R
Hoff P
Häupl T
Hofmann-Amtenbrink M
Poole AR
Hofmann H
Buttgereit F
author_facet Strehl C
Gaber T
Maurizi L
Hahne M
Rauch R
Hoff P
Häupl T
Hofmann-Amtenbrink M
Poole AR
Hofmann H
Buttgereit F
author_sort Strehl C
title Effects of PVA coated nanoparticles on human immune cells
title_short Effects of PVA coated nanoparticles on human immune cells
title_full Effects of PVA coated nanoparticles on human immune cells
title_fullStr Effects of PVA coated nanoparticles on human immune cells
title_full_unstemmed Effects of PVA coated nanoparticles on human immune cells
title_sort effects of pva coated nanoparticles on human immune cells
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/d7e32c3db6cd4d2da5e0fc6bb83b0711
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