Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells. They found that both kinds of editors could induce broad changes in expression and splicing, independent of Cas9 activity, highlighting the...

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Autores principales:	Jiao Fan, Yige Ding, Chao Ren, Ziguo Song, Jie Yuan, Qiuzhen Chen, Chenchen Du, Chao Li, Xiaolong Wang, Wenjie Shu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d7eb1dadf68045f89f9b6517005e2443
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spelling	oai:doaj.org-article:d7eb1dadf68045f89f9b6517005e24432021-12-02T15:32:57ZCytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing10.1038/s42003-021-02406-52399-3642https://doaj.org/article/d7eb1dadf68045f89f9b6517005e24432021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02406-5https://doaj.org/toc/2399-3642Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells. They found that both kinds of editors could induce broad changes in expression and splicing, independent of Cas9 activity, highlighting the need for further efforts to characterize deaminase enzymes in base editor platforms.Jiao FanYige DingChao RenZiguo SongJie YuanQiuzhen ChenChenchen DuChao LiXiaolong WangWenjie ShuNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jiao Fan Yige Ding Chao Ren Ziguo Song Jie Yuan Qiuzhen Chen Chenchen Du Chao Li Xiaolong Wang Wenjie Shu Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
description	Jiao Fan, Yige Ding, et al. examine the impact of cytosine and adenine deaminases on transcriptome-wide gene expression and splicing in HeLa and HEK293T cells. They found that both kinds of editors could induce broad changes in expression and splicing, independent of Cas9 activity, highlighting the need for further efforts to characterize deaminase enzymes in base editor platforms.
format	article
author	Jiao Fan Yige Ding Chao Ren Ziguo Song Jie Yuan Qiuzhen Chen Chenchen Du Chao Li Xiaolong Wang Wenjie Shu
author_facet	Jiao Fan Yige Ding Chao Ren Ziguo Song Jie Yuan Qiuzhen Chen Chenchen Du Chao Li Xiaolong Wang Wenjie Shu
author_sort	Jiao Fan
title	Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
title_short	Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
title_full	Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
title_fullStr	Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
title_full_unstemmed	Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
title_sort	cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7eb1dadf68045f89f9b6517005e2443
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Cytosine and adenine deaminase base-editors induce broad and nonspecific changes in gene expression and splicing

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