An anti vimentin antibody promotes tube formation

Abstract In recent years, there has been an increasing appreciation of the importance of secreted and extracellular proteins that traditionally have been considered as intracellular components. Vimentin is a highly abundant intermediate filament protein, and its intracellular functions have been inv...

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Autores principales:	Mathias Lindh Jørgensen, Carina Kjeldahl Møller, Lasse Rasmussen, Louise Boisen, Henrik Pedersen, Peter Kristensen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d7f4177250e241dfa75409dfdfeca740
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spelling	oai:doaj.org-article:d7f4177250e241dfa75409dfdfeca7402021-12-02T15:05:26ZAn anti vimentin antibody promotes tube formation10.1038/s41598-017-03799-22045-2322https://doaj.org/article/d7f4177250e241dfa75409dfdfeca7402017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03799-2https://doaj.org/toc/2045-2322Abstract In recent years, there has been an increasing appreciation of the importance of secreted and extracellular proteins that traditionally have been considered as intracellular components. Vimentin is a highly abundant intermediate filament protein, and its intracellular functions have been investigated in a large number of studies. Recently, however, vimentin has been shown to take part in significant processes outside the cell. Our understanding of the functions of extracellular vimentin is, however, limited. In this study we demonstrate that a vimentin specific antibody, obtained by phage antibody technology, promotes tube formation of endothelial cells in a 2D matrigel assay. By binding vimentin, the antibody increases the tube formation by 21% after 5 hours of incubation. Addition of the antibody directly to cultured endothelial cells does not influence endothelial cell migration or proliferation. The enhanced tube formation can be seen for up to 10 hours where after the effect decreases. It is shown that the antibody-binding site is located on the coil 2 domain of vimentin. To our knowledge this is the first study that demonstrates an enhanced tube formation by binding vimentin in a 2D matrigel assay under normoxic conditions.Mathias Lindh JørgensenCarina Kjeldahl MøllerLasse RasmussenLouise BoisenHenrik PedersenPeter KristensenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mathias Lindh Jørgensen Carina Kjeldahl Møller Lasse Rasmussen Louise Boisen Henrik Pedersen Peter Kristensen An anti vimentin antibody promotes tube formation
description	Abstract In recent years, there has been an increasing appreciation of the importance of secreted and extracellular proteins that traditionally have been considered as intracellular components. Vimentin is a highly abundant intermediate filament protein, and its intracellular functions have been investigated in a large number of studies. Recently, however, vimentin has been shown to take part in significant processes outside the cell. Our understanding of the functions of extracellular vimentin is, however, limited. In this study we demonstrate that a vimentin specific antibody, obtained by phage antibody technology, promotes tube formation of endothelial cells in a 2D matrigel assay. By binding vimentin, the antibody increases the tube formation by 21% after 5 hours of incubation. Addition of the antibody directly to cultured endothelial cells does not influence endothelial cell migration or proliferation. The enhanced tube formation can be seen for up to 10 hours where after the effect decreases. It is shown that the antibody-binding site is located on the coil 2 domain of vimentin. To our knowledge this is the first study that demonstrates an enhanced tube formation by binding vimentin in a 2D matrigel assay under normoxic conditions.
format	article
author	Mathias Lindh Jørgensen Carina Kjeldahl Møller Lasse Rasmussen Louise Boisen Henrik Pedersen Peter Kristensen
author_facet	Mathias Lindh Jørgensen Carina Kjeldahl Møller Lasse Rasmussen Louise Boisen Henrik Pedersen Peter Kristensen
author_sort	Mathias Lindh Jørgensen
title	An anti vimentin antibody promotes tube formation
title_short	An anti vimentin antibody promotes tube formation
title_full	An anti vimentin antibody promotes tube formation
title_fullStr	An anti vimentin antibody promotes tube formation
title_full_unstemmed	An anti vimentin antibody promotes tube formation
title_sort	anti vimentin antibody promotes tube formation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d7f4177250e241dfa75409dfdfeca740
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An anti vimentin antibody promotes tube formation

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