Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza

Abstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) anti...

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Autores principales:	Angita Shrestha, Jean-Remy Sadeyen, Deimante Lukosaityte, Pengxiang Chang, Adrian Smith, Marielle Van Hulten, Munir Iqbal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/d7ffdce6ab774f3594d034b069050909
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spelling	oai:doaj.org-article:d7ffdce6ab774f3594d034b0690509092021-12-02T18:30:44ZSelectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza10.1038/s41541-021-00350-32059-0105https://doaj.org/article/d7ffdce6ab774f3594d034b0690509092021-07-01T00:00:00Zhttps://doi.org/10.1038/s41541-021-00350-3https://doaj.org/toc/2059-0105Abstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines.Angita ShresthaJean-Remy SadeyenDeimante LukosaitytePengxiang ChangAdrian SmithMarielle Van HultenMunir IqbalNature PortfolioarticleImmunologic diseases. AllergyRC581-607Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENnpj Vaccines, Vol 6, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Immunologic diseases. Allergy RC581-607 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
description	Abstract The immunogenicity and protective efficacy of vaccines can be enhanced by the selective delivery of antigens to the antigen-presenting cells (APCs). In this study, H9N2 avian influenza virus haemagglutinin (HA) antigen, was targeted by fusing it to single-chain fragment variable (scFv) antibodies specific to CD83 receptor expressed on chicken APCs. We observed an increased level of IFNγ, IL6, IL1β, IL4, and CxCLi2 mRNA upon stimulation of chicken splenocytes ex vivo by CD83 scFv targeted H9HA. In addition, CD83 scFv targeted H9HA induced higher serum haemagglutinin inhibition activity and virus neutralising antibodies compared to untargeted H9HA, with induction of antibodies as early as day 6 post primary vaccination. Furthermore, chickens vaccinated with CD83 scFv targeted H9HA showed reduced H9N2 challenge virus shedding compared to untargeted H9HA. These results suggest that targeting antigens to CD83 receptors could improve the efficacy of poultry vaccines.
format	article
author	Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal
author_facet	Angita Shrestha Jean-Remy Sadeyen Deimante Lukosaityte Pengxiang Chang Adrian Smith Marielle Van Hulten Munir Iqbal
author_sort	Angita Shrestha
title	Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_short	Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_full	Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_fullStr	Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_full_unstemmed	Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza
title_sort	selectively targeting haemagglutinin antigen to chicken cd83 receptor induces faster and stronger immunity against avian influenza
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d7ffdce6ab774f3594d034b069050909
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Selectively targeting haemagglutinin antigen to chicken CD83 receptor induces faster and stronger immunity against avian influenza

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