Genome-Wide Identification of Hsp90 Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses

The heat shock protein 90 (Hsp90) is a protein produced in plants in response to stress. This study identified and analyzed Hsp90 gene family members in the perennial ryegrass genome. From the results, eight Hsp90 proteins were obtained and th...

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Autores principales:	Charlotte Appiah, Zhong-Fu Yang, Jie He, Yang Wang, Jie Zhou, Wen-Zhi Xu, Gang Nie, Yong-Qun Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	perennial ryegrass <i>Hsp90</i> abiotic stress expression profiles phylogenetic analysis Botany QK1-989
Acceso en línea:	https://doaj.org/article/d800e965dc53497db9de5ed77e0f91f0
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spelling	oai:doaj.org-article:d800e965dc53497db9de5ed77e0f91f02021-11-25T18:47:11ZGenome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses10.3390/plants101125092223-7747https://doaj.org/article/d800e965dc53497db9de5ed77e0f91f02021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2509https://doaj.org/toc/2223-7747The heat shock protein 90 (<i>Hsp90</i>) is a protein produced in plants in response to stress. This study identified and analyzed <i>Hsp90</i> gene family members in the perennial ryegrass genome. From the results, eight <i>Hsp90</i> proteins were obtained and their MW, pI and number of amino acid bases varied. The amino acid bases ranged from 526 to 862. The CDS also ranged from 20 (<i>LpHsp0-4</i>) to 1 <i>(LpHsp90-5</i>). The least number of CDS regions was 1 (<i>LpHsp90-5</i>) with 528 kb amino acids, while the highest was 20 (<i>LpHsp90-4</i>) with 862 kb amino acids, which showed diversity among the protein sequences. The phylogenetic tree revealed that <i>Hsp90</i> genes in <i>Lolium perenne</i>, <i>Arabidopsis thaliana</i>, <i>Oryza sativa</i> and <i>Brachypodium distachyon</i> could be divided into two groups with five paralogous gene pairs and three orthologous gene pairs. The expression analysis after perennial ryegrass was subjected to heat, salt, chromium (Cr), cadmium (Cd), polyethylene glycol (PEG) and abscisic acid (ABA) revealed that <i>LpHsp90</i> genes were generally highly expressed under heat stress, but only two <i>LpHsp90</i> proteins were expressed under Cr stresses. Additionally, the expression of the <i>LpHsp90</i> proteins differed at each time point in all treatments. This study provides the basis for an understanding of the functions of <i>LpHsp90</i> proteins in abiotic stress studies and in plant breeding.Charlotte AppiahZhong-Fu YangJie HeYang WangJie ZhouWen-Zhi XuGang NieYong-Qun ZhuMDPI AGarticleperennial ryegrass<i>Hsp90</i>abiotic stressexpression profilesphylogenetic analysisBotanyQK1-989ENPlants, Vol 10, Iss 2509, p 2509 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	perennial ryegrass <i>Hsp90</i> abiotic stress expression profiles phylogenetic analysis Botany QK1-989
spellingShingle	perennial ryegrass <i>Hsp90</i> abiotic stress expression profiles phylogenetic analysis Botany QK1-989 Charlotte Appiah Zhong-Fu Yang Jie He Yang Wang Jie Zhou Wen-Zhi Xu Gang Nie Yong-Qun Zhu Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
description	The heat shock protein 90 (<i>Hsp90</i>) is a protein produced in plants in response to stress. This study identified and analyzed <i>Hsp90</i> gene family members in the perennial ryegrass genome. From the results, eight <i>Hsp90</i> proteins were obtained and their MW, pI and number of amino acid bases varied. The amino acid bases ranged from 526 to 862. The CDS also ranged from 20 (<i>LpHsp0-4</i>) to 1 <i>(LpHsp90-5</i>). The least number of CDS regions was 1 (<i>LpHsp90-5</i>) with 528 kb amino acids, while the highest was 20 (<i>LpHsp90-4</i>) with 862 kb amino acids, which showed diversity among the protein sequences. The phylogenetic tree revealed that <i>Hsp90</i> genes in <i>Lolium perenne</i>, <i>Arabidopsis thaliana</i>, <i>Oryza sativa</i> and <i>Brachypodium distachyon</i> could be divided into two groups with five paralogous gene pairs and three orthologous gene pairs. The expression analysis after perennial ryegrass was subjected to heat, salt, chromium (Cr), cadmium (Cd), polyethylene glycol (PEG) and abscisic acid (ABA) revealed that <i>LpHsp90</i> genes were generally highly expressed under heat stress, but only two <i>LpHsp90</i> proteins were expressed under Cr stresses. Additionally, the expression of the <i>LpHsp90</i> proteins differed at each time point in all treatments. This study provides the basis for an understanding of the functions of <i>LpHsp90</i> proteins in abiotic stress studies and in plant breeding.
format	article
author	Charlotte Appiah Zhong-Fu Yang Jie He Yang Wang Jie Zhou Wen-Zhi Xu Gang Nie Yong-Qun Zhu
author_facet	Charlotte Appiah Zhong-Fu Yang Jie He Yang Wang Jie Zhou Wen-Zhi Xu Gang Nie Yong-Qun Zhu
author_sort	Charlotte Appiah
title	Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
title_short	Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
title_full	Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
title_fullStr	Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
title_full_unstemmed	Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses
title_sort	genome-wide identification of <i>hsp90</i> gene family in perennial ryegrass and expression analysis under various abiotic stresses
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d800e965dc53497db9de5ed77e0f91f0
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Genome-Wide Identification of <i>Hsp90</i> Gene Family in Perennial Ryegrass and Expression Analysis under Various Abiotic Stresses

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