Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation

Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approache...

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Autores principales:	Anita Horvatić, Andrea Gelemanović, Boris Pirkić, Ozren Smolec, Blanka Beer Ljubić, Ivana Rubić, Peter David Eckersall, Vladimir Mrljak, Mark McLaughlin, Marko Samardžija, Marija Lipar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cerebrospinal fluid dog integromics intervertebral disc herniation metabolomics proteomics Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d8029307302545bc999d5ec4a090294c
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spelling	oai:doaj.org-article:d8029307302545bc999d5ec4a090294c2021-11-11T17:08:27ZMulti-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation10.3390/ijms2221116781422-00671661-6596https://doaj.org/article/d8029307302545bc999d5ec4a090294c2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11678https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including high-resolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptor-stimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders.Anita HorvatićAndrea GelemanovićBoris PirkićOzren SmolecBlanka Beer LjubićIvana RubićPeter David EckersallVladimir MrljakMark McLaughlinMarko SamardžijaMarija LiparMDPI AGarticlecerebrospinal fluiddogintegromicsintervertebral disc herniationmetabolomicsproteomicsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11678, p 11678 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cerebrospinal fluid dog integromics intervertebral disc herniation metabolomics proteomics Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cerebrospinal fluid dog integromics intervertebral disc herniation metabolomics proteomics Biology (General) QH301-705.5 Chemistry QD1-999 Anita Horvatić Andrea Gelemanović Boris Pirkić Ozren Smolec Blanka Beer Ljubić Ivana Rubić Peter David Eckersall Vladimir Mrljak Mark McLaughlin Marko Samardžija Marija Lipar Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
description	Herniation of the intervertebral disc (IVDH) is the most common cause of neurological and intervertebral disc degeneration-related diseases. Since the disc starts to degenerate before it can be observed by currently available diagnostic methods, there is an urgent need for novel diagnostic approaches. To identify molecular networks and pathways which may play important roles in intervertebral disc herniation, as well as to reveal the potential features which could be useful for monitoring disease progression and prognosis, multi-omics profiling, including high-resolution liquid chromatography-mass spectrometry (LC-MS)-based metabolomics and tandem mass tag (TMT)-based proteomics was performed. Cerebrospinal fluid of nine dogs with IVDH and six healthy controls were used for the analyses, and an additional five IVDH samples were used for proteomic data validation. Furthermore, multi-omics data were integrated to decipher a complex interaction between individual omics layers, leading to an improved prediction model. Together with metabolic pathways related to amino acids and lipid metabolism and coagulation cascades, our integromics prediction model identified the key features in IVDH, namely the proteins follistatin Like 1 (FSTL1), secretogranin V (SCG5), nucleobindin 1 (NUCB1), calcitonin re-ceptor-stimulating peptide 2 precursor (CRSP2) and the metabolites N-acetyl-D-glucosamine and adenine, involved in neuropathic pain, myelination, and neurotransmission and inflammatory response, respectively. Their clinical application is to be further investigated. The utilization of a novel integrative interdisciplinary approach may provide new opportunities to apply innovative diagnostic and monitoring methods as well as improve treatment strategies and personalized care for patients with degenerative spinal disorders.
format	article
author	Anita Horvatić Andrea Gelemanović Boris Pirkić Ozren Smolec Blanka Beer Ljubić Ivana Rubić Peter David Eckersall Vladimir Mrljak Mark McLaughlin Marko Samardžija Marija Lipar
author_facet	Anita Horvatić Andrea Gelemanović Boris Pirkić Ozren Smolec Blanka Beer Ljubić Ivana Rubić Peter David Eckersall Vladimir Mrljak Mark McLaughlin Marko Samardžija Marija Lipar
author_sort	Anita Horvatić
title	Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
title_short	Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
title_full	Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
title_fullStr	Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
title_full_unstemmed	Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation
title_sort	multi-omics approach to elucidate cerebrospinal fluid changes in dogs with intervertebral disc herniation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d8029307302545bc999d5ec4a090294c
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Multi-Omics Approach to Elucidate Cerebrospinal Fluid Changes in Dogs with Intervertebral Disc Herniation

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