Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients

The latest literature demonstrates the predominant role of the programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate the clinical importance of PD-L1/PD-L2 expression in OC environments. We...

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Autores principales:	Anna Pawłowska, Agnieszka Kwiatkowska, Dorota Suszczyk, Agata Chudzik, Rafał Tarkowski, Bartłomiej Barczyński, Jan Kotarski, Iwona Wertel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ovarian cancer tumor microenvironment immune suppression programmed cell death pathway immunotherapy Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d80945de717c459d87c4f56c8d33cfdb
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spelling	oai:doaj.org-article:d80945de717c459d87c4f56c8d33cfdb2021-11-11T17:02:44ZClinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients10.3390/ijms2221115631422-00671661-6596https://doaj.org/article/d80945de717c459d87c4f56c8d33cfdb2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11563https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The latest literature demonstrates the predominant role of the programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate the clinical importance of PD-L1/PD-L2 expression in OC environments. We evaluated the role of PD-L1/PD-L2 in OC patients (<i>n</i> = 53). The analysis was performed via flow cytometry on myeloid (mDCs) and plasmacytoid dendritic cells (pDCs) and monocytes/macrophages (MO/MA) in peripheral blood, peritoneal fluid (PF), and tumor tissue (TT). The data were correlated with clinicopathological characteristics and prognosis of OC patients. The concentration of soluble PD-L1 (sPD-L1) and PD-1 in the plasma and PF were determined by ELISA. We established an accumulation of PD-L1<sup>+</sup>/PD-L2<sup>+</sup> mDCs, pDCs, and MA in the tumor microenvironment. We showed an elevated level of sPD-L1 in the PF of OC patients in comparison to plasma and healthy subjects. sPD-L1 levels in PF showed a positive relationship with Ca125 concentration. Moreover, we established an association between higher sPD-L1 levels in PF and shorter survival of OC patients. An accumulation of PD-L1<sup>+</sup>/PD-L2<sup>+</sup> mDCs, pDCs, and MA in the TT and high sPD-L1 levels in PF could represent the hallmark of immune regulation in OC patients.Anna PawłowskaAgnieszka KwiatkowskaDorota SuszczykAgata ChudzikRafał TarkowskiBartłomiej BarczyńskiJan KotarskiIwona WertelMDPI AGarticleovarian cancertumor microenvironmentimmune suppressionprogrammed cell death pathwayimmunotherapyBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11563, p 11563 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovarian cancer tumor microenvironment immune suppression programmed cell death pathway immunotherapy Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ovarian cancer tumor microenvironment immune suppression programmed cell death pathway immunotherapy Biology (General) QH301-705.5 Chemistry QD1-999 Anna Pawłowska Agnieszka Kwiatkowska Dorota Suszczyk Agata Chudzik Rafał Tarkowski Bartłomiej Barczyński Jan Kotarski Iwona Wertel Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
description	The latest literature demonstrates the predominant role of the programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate the clinical importance of PD-L1/PD-L2 expression in OC environments. We evaluated the role of PD-L1/PD-L2 in OC patients (<i>n</i> = 53). The analysis was performed via flow cytometry on myeloid (mDCs) and plasmacytoid dendritic cells (pDCs) and monocytes/macrophages (MO/MA) in peripheral blood, peritoneal fluid (PF), and tumor tissue (TT). The data were correlated with clinicopathological characteristics and prognosis of OC patients. The concentration of soluble PD-L1 (sPD-L1) and PD-1 in the plasma and PF were determined by ELISA. We established an accumulation of PD-L1<sup>+</sup>/PD-L2<sup>+</sup> mDCs, pDCs, and MA in the tumor microenvironment. We showed an elevated level of sPD-L1 in the PF of OC patients in comparison to plasma and healthy subjects. sPD-L1 levels in PF showed a positive relationship with Ca125 concentration. Moreover, we established an association between higher sPD-L1 levels in PF and shorter survival of OC patients. An accumulation of PD-L1<sup>+</sup>/PD-L2<sup>+</sup> mDCs, pDCs, and MA in the TT and high sPD-L1 levels in PF could represent the hallmark of immune regulation in OC patients.
format	article
author	Anna Pawłowska Agnieszka Kwiatkowska Dorota Suszczyk Agata Chudzik Rafał Tarkowski Bartłomiej Barczyński Jan Kotarski Iwona Wertel
author_facet	Anna Pawłowska Agnieszka Kwiatkowska Dorota Suszczyk Agata Chudzik Rafał Tarkowski Bartłomiej Barczyński Jan Kotarski Iwona Wertel
author_sort	Anna Pawłowska
title	Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
title_short	Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
title_full	Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
title_fullStr	Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
title_full_unstemmed	Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients
title_sort	clinical and prognostic value of antigen-presenting cells with pd-l1/pd-l2 expression in ovarian cancer patients
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d80945de717c459d87c4f56c8d33cfdb
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Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients

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