3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.

Oxidative stress induces many serious reproductive diseases in female mammals and thus poses a serious threat to reproductive health. However, the relationship between reactive oxygen species (ROS)-induced oxidative stress and follicular development, oocyte and embryo quality is not clear. The aim o...

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Autores principales:	Jia-Qing Zhang, Ming Shen, Cheng-Cheng Zhu, Feng-Xiang Yu, Ze-Qun Liu, Nazim Ally, Shao-Chen Sun, Kui Li, Hong-Lin Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d80cce757a29487ab54e81d9bff65d31
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spelling	oai:doaj.org-article:d80cce757a29487ab54e81d9bff65d312021-11-18T08:33:48Z3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.1932-620310.1371/journal.pone.0086589https://doaj.org/article/d80cce757a29487ab54e81d9bff65d312014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505260/?tool=EBIhttps://doaj.org/toc/1932-6203Oxidative stress induces many serious reproductive diseases in female mammals and thus poses a serious threat to reproductive health. However, the relationship between reactive oxygen species (ROS)-induced oxidative stress and follicular development, oocyte and embryo quality is not clear. The aim of this study was to investigate the effect of ovarian oxidative stress on the health of follicle and oocyte development. Female ICR mice were dosed with 3-nitropropionic acid (3-NPA) at three different concentrations (6.25, 12.5 and 25 mg/kg) and saline (control) via continuous intraperitoneal injection for 7 days. The treatment with 12.5 mg/kg reduced the weight of mouse ovaries, and significantly increased ROS levels and the activities of antioxidant enzymes--total superoxide dismutase (T-SOD), glutathione peroxidase (GPx) and catalase (CAT)--in granulosa cells and ovarian tissues, but not in other tissues (brain, liver, kidney and spleen). The same treatment significantly increased the percentage of atretic large follicles, and reduced the number of large follicles, the number of ovulated oocytes, and the capacity for early embryonic development compared with controls. It also significantly decreased the ratio of Bcl-2 to Bax, while causing an increase in the mRNA expression of (SOD2, CAT and GP X) and ROS levels in granulosa cells. Collectively, these data indicate that 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary. Therefore, we have established an in vivo model of ovarian oxidative stress for studying the mechanism of resulting damage induced by free radicals and for the screening of novel antioxidants.Jia-Qing ZhangMing ShenCheng-Cheng ZhuFeng-Xiang YuZe-Qun LiuNazim AllyShao-Chen SunKui LiHong-Lin LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e86589 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jia-Qing Zhang Ming Shen Cheng-Cheng Zhu Feng-Xiang Yu Ze-Qun Liu Nazim Ally Shao-Chen Sun Kui Li Hong-Lin Liu 3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
description	Oxidative stress induces many serious reproductive diseases in female mammals and thus poses a serious threat to reproductive health. However, the relationship between reactive oxygen species (ROS)-induced oxidative stress and follicular development, oocyte and embryo quality is not clear. The aim of this study was to investigate the effect of ovarian oxidative stress on the health of follicle and oocyte development. Female ICR mice were dosed with 3-nitropropionic acid (3-NPA) at three different concentrations (6.25, 12.5 and 25 mg/kg) and saline (control) via continuous intraperitoneal injection for 7 days. The treatment with 12.5 mg/kg reduced the weight of mouse ovaries, and significantly increased ROS levels and the activities of antioxidant enzymes--total superoxide dismutase (T-SOD), glutathione peroxidase (GPx) and catalase (CAT)--in granulosa cells and ovarian tissues, but not in other tissues (brain, liver, kidney and spleen). The same treatment significantly increased the percentage of atretic large follicles, and reduced the number of large follicles, the number of ovulated oocytes, and the capacity for early embryonic development compared with controls. It also significantly decreased the ratio of Bcl-2 to Bax, while causing an increase in the mRNA expression of (SOD2, CAT and GP X) and ROS levels in granulosa cells. Collectively, these data indicate that 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary. Therefore, we have established an in vivo model of ovarian oxidative stress for studying the mechanism of resulting damage induced by free radicals and for the screening of novel antioxidants.
format	article
author	Jia-Qing Zhang Ming Shen Cheng-Cheng Zhu Feng-Xiang Yu Ze-Qun Liu Nazim Ally Shao-Chen Sun Kui Li Hong-Lin Liu
author_facet	Jia-Qing Zhang Ming Shen Cheng-Cheng Zhu Feng-Xiang Yu Ze-Qun Liu Nazim Ally Shao-Chen Sun Kui Li Hong-Lin Liu
author_sort	Jia-Qing Zhang
title	3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
title_short	3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
title_full	3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
title_fullStr	3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
title_full_unstemmed	3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
title_sort	3-nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/d80cce757a29487ab54e81d9bff65d31
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3-Nitropropionic acid induces ovarian oxidative stress and impairs follicle in mouse.

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