Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy

The treatment of bone diseases (including osteoporosis, osteoarthritis, and bone cancer) often results in reduced efficiency and/or adverse reactions due to the fact that it is not specifically targeted to the site of action. The employment of a suitable carrier should increase drug location to the...

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Autores principales:	Simona Sapino, Giulia Chindamo, Daniela Chirio, Maela Manzoli, Elena Peira, Chiara Riganti, Marina Gallarate
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lipid nanoparticles calcium phosphate coating bone diseases human osteosarcoma cells cellular uptake Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/d8112b6d9e3342b1ba5f3fabff378314
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spelling	oai:doaj.org-article:d8112b6d9e3342b1ba5f3fabff3783142021-11-25T18:31:24ZCalcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy10.3390/nano111129832079-4991https://doaj.org/article/d8112b6d9e3342b1ba5f3fabff3783142021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2983https://doaj.org/toc/2079-4991The treatment of bone diseases (including osteoporosis, osteoarthritis, and bone cancer) often results in reduced efficiency and/or adverse reactions due to the fact that it is not specifically targeted to the site of action. The employment of a suitable carrier should increase drug location to the site of bone disease. The purpose of this study is to prepare and characterize lipid nanoparticles (NPs) coated with calcium phosphate (CaP-NPs). A coating method, to date used only to obtain liposomes covered with CaP, is herein partially-modified to prepare CaP-coated lipid NPs. An extensive physico-chemical characterization was achieved by employing several techniques (DLS, SEM and TEM, and both combined with EDS, XRD, and FTIR) that confirmed the feasibility of the developed coating method. Preliminary uptake studies on human osteosarcoma cells (U-2OS) were performed by entrapping, as a lipid probe, Sudan Red III in NPs. The obtained data provided evidence that CaP-NPs showed higher cell accumulation than uncoated NPs. This result may have important implications for the development of drug loaded CaP-NPs to be tested in vitro with a view of planning future treatment of bone diseases, and indicate that CaP-NPs are potential vehicles for selective drug delivery to bone tissue.Simona SapinoGiulia ChindamoDaniela ChirioMaela ManzoliElena PeiraChiara RigantiMarina GallarateMDPI AGarticlelipid nanoparticlescalcium phosphate coatingbone diseaseshuman osteosarcoma cellscellular uptakeChemistryQD1-999ENNanomaterials, Vol 11, Iss 2983, p 2983 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid nanoparticles calcium phosphate coating bone diseases human osteosarcoma cells cellular uptake Chemistry QD1-999
spellingShingle	lipid nanoparticles calcium phosphate coating bone diseases human osteosarcoma cells cellular uptake Chemistry QD1-999 Simona Sapino Giulia Chindamo Daniela Chirio Maela Manzoli Elena Peira Chiara Riganti Marina Gallarate Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
description	The treatment of bone diseases (including osteoporosis, osteoarthritis, and bone cancer) often results in reduced efficiency and/or adverse reactions due to the fact that it is not specifically targeted to the site of action. The employment of a suitable carrier should increase drug location to the site of bone disease. The purpose of this study is to prepare and characterize lipid nanoparticles (NPs) coated with calcium phosphate (CaP-NPs). A coating method, to date used only to obtain liposomes covered with CaP, is herein partially-modified to prepare CaP-coated lipid NPs. An extensive physico-chemical characterization was achieved by employing several techniques (DLS, SEM and TEM, and both combined with EDS, XRD, and FTIR) that confirmed the feasibility of the developed coating method. Preliminary uptake studies on human osteosarcoma cells (U-2OS) were performed by entrapping, as a lipid probe, Sudan Red III in NPs. The obtained data provided evidence that CaP-NPs showed higher cell accumulation than uncoated NPs. This result may have important implications for the development of drug loaded CaP-NPs to be tested in vitro with a view of planning future treatment of bone diseases, and indicate that CaP-NPs are potential vehicles for selective drug delivery to bone tissue.
format	article
author	Simona Sapino Giulia Chindamo Daniela Chirio Maela Manzoli Elena Peira Chiara Riganti Marina Gallarate
author_facet	Simona Sapino Giulia Chindamo Daniela Chirio Maela Manzoli Elena Peira Chiara Riganti Marina Gallarate
author_sort	Simona Sapino
title	Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
title_short	Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
title_full	Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
title_fullStr	Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
title_full_unstemmed	Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy
title_sort	calcium phosphate-coated lipid nanoparticles as a potential tool in bone diseases therapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d8112b6d9e3342b1ba5f3fabff378314
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Calcium Phosphate-Coated Lipid Nanoparticles as a Potential Tool in Bone Diseases Therapy

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