Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats

Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats....

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Autores principales:	Basiru O. Ajiboye, Babatunji E. Oyinloye, Precious E. Agboinghale, Sunday A. Onikanni, Emeka Asogwa, Abidemi P. Kappo
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	diabetes mellitus ethnobotanical doses intraperitoneal hexokinase; glut-2 glut-4 lipid peroxidation Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d819217880734f948cd73a58507a0426
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spelling	oai:doaj.org-article:d819217880734f948cd73a58507a04262021-11-17T14:21:56ZAntihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats1388-02091744-511610.1080/13880209.2019.1657907https://doaj.org/article/d819217880734f948cd73a58507a04262019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1657907https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats. Materials and methods: Forty-eight female Wistar rats were induced intraperitoneally using alloxan (150 mg/kg body weight). The rats were separated into six groups (n = 8) as follows: non-diabetic control, diabetic control, diabetic rats administered 5 mg/kg body weight of metformin, and diabetic rats administered 6.36, 12.72 and 25.44 mg/kg body weight (ethnobotanical doses) of G. latifolium orally daily. On the 14th day, the animals were sacrificed and different antihyperglycaemic parameters were evaluated as well as its related gene expressions. Results: Diabetic rats administered three doses of aqueous extract of G. latifolium significantly (p < 0.05) lowered the fasting blood glucose, glycated haemoglobin, serum lipid profiles, lipid peroxidation (5.62–1.2 μ/mg protein) levels, as well as gene expression of glucose-6-phosphatase in alloxan-induced diabetic rats. There was a significant (p < 0.05) increase in the liver glycogen content (16.23–112.5 mg glucose/2 g), antioxidant enzymes activities, glucose transporter (GLUT-2 and GLUT-4) levels and relative gene expression of hexokinase in diabetic rats administered different doses of aqueous extract of G. latifolium. Discussion and conclusions: It can be deduced that the aqueous extract of G. latifolium leaf at these doses may be useful in managing diabetes mellitus and its associated complications. Therefore, this extract may be a potent antidiabetic agent in clinical therapy in the future.Basiru O. AjiboyeBabatunji E. OyinloyePrecious E. AgboinghaleSunday A. OnikanniEmeka AsogwaAbidemi P. KappoTaylor & Francis Grouparticlediabetes mellitusethnobotanical dosesintraperitonealhexokinase; glut-2glut-4lipid peroxidationTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 604-611 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes mellitus ethnobotanical doses intraperitoneal hexokinase; glut-2 glut-4 lipid peroxidation Therapeutics. Pharmacology RM1-950
spellingShingle	diabetes mellitus ethnobotanical doses intraperitoneal hexokinase; glut-2 glut-4 lipid peroxidation Therapeutics. Pharmacology RM1-950 Basiru O. Ajiboye Babatunji E. Oyinloye Precious E. Agboinghale Sunday A. Onikanni Emeka Asogwa Abidemi P. Kappo Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
description	Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats. Materials and methods: Forty-eight female Wistar rats were induced intraperitoneally using alloxan (150 mg/kg body weight). The rats were separated into six groups (n = 8) as follows: non-diabetic control, diabetic control, diabetic rats administered 5 mg/kg body weight of metformin, and diabetic rats administered 6.36, 12.72 and 25.44 mg/kg body weight (ethnobotanical doses) of G. latifolium orally daily. On the 14th day, the animals were sacrificed and different antihyperglycaemic parameters were evaluated as well as its related gene expressions. Results: Diabetic rats administered three doses of aqueous extract of G. latifolium significantly (p < 0.05) lowered the fasting blood glucose, glycated haemoglobin, serum lipid profiles, lipid peroxidation (5.62–1.2 μ/mg protein) levels, as well as gene expression of glucose-6-phosphatase in alloxan-induced diabetic rats. There was a significant (p < 0.05) increase in the liver glycogen content (16.23–112.5 mg glucose/2 g), antioxidant enzymes activities, glucose transporter (GLUT-2 and GLUT-4) levels and relative gene expression of hexokinase in diabetic rats administered different doses of aqueous extract of G. latifolium. Discussion and conclusions: It can be deduced that the aqueous extract of G. latifolium leaf at these doses may be useful in managing diabetes mellitus and its associated complications. Therefore, this extract may be a potent antidiabetic agent in clinical therapy in the future.
format	article
author	Basiru O. Ajiboye Babatunji E. Oyinloye Precious E. Agboinghale Sunday A. Onikanni Emeka Asogwa Abidemi P. Kappo
author_facet	Basiru O. Ajiboye Babatunji E. Oyinloye Precious E. Agboinghale Sunday A. Onikanni Emeka Asogwa Abidemi P. Kappo
author_sort	Basiru O. Ajiboye
title	Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_short	Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_full	Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_fullStr	Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_full_unstemmed	Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_sort	antihyperglycaemia and related gene expressions of aqueous extract of gongronema latifolium leaf in alloxan-induced diabetic rats
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/d819217880734f948cd73a58507a0426
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Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats

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