Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats

Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats....

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Basiru O. Ajiboye, Babatunji E. Oyinloye, Precious E. Agboinghale, Sunday A. Onikanni, Emeka Asogwa, Abidemi P. Kappo
Formato: article
Lenguaje:EN
Publicado: Taylor & Francis Group 2019
Materias:
Acceso en línea:https://doaj.org/article/d819217880734f948cd73a58507a0426
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:d819217880734f948cd73a58507a0426
record_format dspace
spelling oai:doaj.org-article:d819217880734f948cd73a58507a04262021-11-17T14:21:56ZAntihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats1388-02091744-511610.1080/13880209.2019.1657907https://doaj.org/article/d819217880734f948cd73a58507a04262019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1657907https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats. Materials and methods: Forty-eight female Wistar rats were induced intraperitoneally using alloxan (150 mg/kg body weight). The rats were separated into six groups (n = 8) as follows: non-diabetic control, diabetic control, diabetic rats administered 5 mg/kg body weight of metformin, and diabetic rats administered 6.36, 12.72 and 25.44 mg/kg body weight (ethnobotanical doses) of G. latifolium orally daily. On the 14th day, the animals were sacrificed and different antihyperglycaemic parameters were evaluated as well as its related gene expressions. Results: Diabetic rats administered three doses of aqueous extract of G. latifolium significantly (p < 0.05) lowered the fasting blood glucose, glycated haemoglobin, serum lipid profiles, lipid peroxidation (5.62–1.2 μ/mg protein) levels, as well as gene expression of glucose-6-phosphatase in alloxan-induced diabetic rats. There was a significant (p < 0.05) increase in the liver glycogen content (16.23–112.5 mg glucose/2 g), antioxidant enzymes activities, glucose transporter (GLUT-2 and GLUT-4) levels and relative gene expression of hexokinase in diabetic rats administered different doses of aqueous extract of G. latifolium. Discussion and conclusions: It can be deduced that the aqueous extract of G. latifolium leaf at these doses may be useful in managing diabetes mellitus and its associated complications. Therefore, this extract may be a potent antidiabetic agent in clinical therapy in the future.Basiru O. AjiboyeBabatunji E. OyinloyePrecious E. AgboinghaleSunday A. OnikanniEmeka AsogwaAbidemi P. KappoTaylor & Francis Grouparticlediabetes mellitusethnobotanical dosesintraperitonealhexokinase; glut-2glut-4lipid peroxidationTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 604-611 (2019)
institution DOAJ
collection DOAJ
language EN
topic diabetes mellitus
ethnobotanical doses
intraperitoneal
hexokinase; glut-2
glut-4
lipid peroxidation
Therapeutics. Pharmacology
RM1-950
spellingShingle diabetes mellitus
ethnobotanical doses
intraperitoneal
hexokinase; glut-2
glut-4
lipid peroxidation
Therapeutics. Pharmacology
RM1-950
Basiru O. Ajiboye
Babatunji E. Oyinloye
Precious E. Agboinghale
Sunday A. Onikanni
Emeka Asogwa
Abidemi P. Kappo
Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
description Context: Gongronema latifolium Benth (Asclepiadaceae) has been highly utilized in controlling diabetes mellitus traditionally in the eastern part of Nigeria. Objectives: Antihyperglycaemic and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats. Materials and methods: Forty-eight female Wistar rats were induced intraperitoneally using alloxan (150 mg/kg body weight). The rats were separated into six groups (n = 8) as follows: non-diabetic control, diabetic control, diabetic rats administered 5 mg/kg body weight of metformin, and diabetic rats administered 6.36, 12.72 and 25.44 mg/kg body weight (ethnobotanical doses) of G. latifolium orally daily. On the 14th day, the animals were sacrificed and different antihyperglycaemic parameters were evaluated as well as its related gene expressions. Results: Diabetic rats administered three doses of aqueous extract of G. latifolium significantly (p < 0.05) lowered the fasting blood glucose, glycated haemoglobin, serum lipid profiles, lipid peroxidation (5.62–1.2 μ/mg protein) levels, as well as gene expression of glucose-6-phosphatase in alloxan-induced diabetic rats. There was a significant (p < 0.05) increase in the liver glycogen content (16.23–112.5 mg glucose/2 g), antioxidant enzymes activities, glucose transporter (GLUT-2 and GLUT-4) levels and relative gene expression of hexokinase in diabetic rats administered different doses of aqueous extract of G. latifolium. Discussion and conclusions: It can be deduced that the aqueous extract of G. latifolium leaf at these doses may be useful in managing diabetes mellitus and its associated complications. Therefore, this extract may be a potent antidiabetic agent in clinical therapy in the future.
format article
author Basiru O. Ajiboye
Babatunji E. Oyinloye
Precious E. Agboinghale
Sunday A. Onikanni
Emeka Asogwa
Abidemi P. Kappo
author_facet Basiru O. Ajiboye
Babatunji E. Oyinloye
Precious E. Agboinghale
Sunday A. Onikanni
Emeka Asogwa
Abidemi P. Kappo
author_sort Basiru O. Ajiboye
title Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_short Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_full Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_fullStr Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_full_unstemmed Antihyperglycaemia and related gene expressions of aqueous extract of Gongronema latifolium leaf in alloxan-induced diabetic rats
title_sort antihyperglycaemia and related gene expressions of aqueous extract of gongronema latifolium leaf in alloxan-induced diabetic rats
publisher Taylor & Francis Group
publishDate 2019
url https://doaj.org/article/d819217880734f948cd73a58507a0426
work_keys_str_mv AT basiruoajiboye antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
AT babatunjieoyinloye antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
AT preciouseagboinghale antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
AT sundayaonikanni antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
AT emekaasogwa antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
AT abidemipkappo antihyperglycaemiaandrelatedgeneexpressionsofaqueousextractofgongronemalatifoliumleafinalloxaninduceddiabeticrats
_version_ 1718425513081438208