Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

Secondary metabolites are organic compounds that can be found in both fungi and plants, where they play an important role as defensive and signal molecules, or provide other kinds of advantage in natural selection, but are not directly involved in normal growth, development and reproduction...

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Autores principales:	Nejc Thaler, Marko Bajc
Formato:	article
Lenguaje:	DE EN ES FR SL
Publicado:	Slovenian Forestry Institute 2013
Materias:	Forestry SD1-669.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/d81dd2cfd77b4ac291f76108d3090a3e
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spelling	oai:doaj.org-article:d81dd2cfd77b4ac291f76108d3090a3e2021-11-15T12:33:09ZVpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)2335-31122335-395310.20315/ASetL.100.3https://doaj.org/article/d81dd2cfd77b4ac291f76108d3090a3e2013-01-01T00:00:00Zhttp://dirros.openscience.si/IzpisGradiva.php?id=7762https://doaj.org/toc/2335-3112https://doaj.org/toc/2335-3953 Secondary metabolites are organic compounds that can be found in both fungi and plants, where they play an important role as defensive and signal molecules, or provide other kinds of advantage in natural selection, but are not directly involved in normal growth, development and reproduction of an organism. When working with DNA techniques, it is the secondary metabolites that most often affect the efficiency of polymerase chain reaction (PCR), either by hindering cell lysis, causing decomposition of nucleic acids or by direct inhibition of DNA-polymerase or reverse transcriptase amplification. The main limiting factor in the application of the PCR technique in routine diagnosis is the preparation of good quality nucleic acids isolates, free of PCR inhibitors. This is especially true in the case of woody plants (Minafra et al., 1992) and soil samples (Tsai and Olson, 1991). Most standard nucleic acids extraction procedures do not always remove contaminating plant polysaccharides or polyphenolic compounds, which can have direct inhibitory effects on subsequent PCR amplification (Demeke and Adams, 1992). Attempts to overcome these limitations included the development of more elaborate nucleic acids extraction methods and PCR, which employ PCR enhancers to eliminate or attenuate the effects of inhibitors. This review is concentrated on removal or attenuation of effects of plant and fungal secondary metabolites from soil, different plant tissues and decayed wood samples due to the significance of this type of research in forestry and wood science.Nejc ThalerMarko BajcSlovenian Forestry InstitutearticleForestrySD1-669.5Environmental sciencesGE1-350DEENESFRSLActa Silvae et Ligni, Vol 100, Pp 25-40 (2013)
institution	DOAJ
collection	DOAJ
language	DE EN ES FR SL
topic	Forestry SD1-669.5 Environmental sciences GE1-350
spellingShingle	Forestry SD1-669.5 Environmental sciences GE1-350 Nejc Thaler Marko Bajc Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
description	Secondary metabolites are organic compounds that can be found in both fungi and plants, where they play an important role as defensive and signal molecules, or provide other kinds of advantage in natural selection, but are not directly involved in normal growth, development and reproduction of an organism. When working with DNA techniques, it is the secondary metabolites that most often affect the efficiency of polymerase chain reaction (PCR), either by hindering cell lysis, causing decomposition of nucleic acids or by direct inhibition of DNA-polymerase or reverse transcriptase amplification. The main limiting factor in the application of the PCR technique in routine diagnosis is the preparation of good quality nucleic acids isolates, free of PCR inhibitors. This is especially true in the case of woody plants (Minafra et al., 1992) and soil samples (Tsai and Olson, 1991). Most standard nucleic acids extraction procedures do not always remove contaminating plant polysaccharides or polyphenolic compounds, which can have direct inhibitory effects on subsequent PCR amplification (Demeke and Adams, 1992). Attempts to overcome these limitations included the development of more elaborate nucleic acids extraction methods and PCR, which employ PCR enhancers to eliminate or attenuate the effects of inhibitors. This review is concentrated on removal or attenuation of effects of plant and fungal secondary metabolites from soil, different plant tissues and decayed wood samples due to the significance of this type of research in forestry and wood science.
format	article
author	Nejc Thaler Marko Bajc
author_facet	Nejc Thaler Marko Bajc
author_sort	Nejc Thaler
title	Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
title_short	Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
title_full	Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
title_fullStr	Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
title_full_unstemmed	Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)
title_sort	vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (pcr)
publisher	Slovenian Forestry Institute
publishDate	2013
url	https://doaj.org/article/d81dd2cfd77b4ac291f76108d3090a3e
work_keys_str_mv	AT nejcthaler vplivglivnihinrastlinskihsekundarnihmetabolitovnaveriznoreakcijospolimerazopcr AT markobajc vplivglivnihinrastlinskihsekundarnihmetabolitovnaveriznoreakcijospolimerazopcr
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Vpliv glivnih in rastlinskih sekundarnih metabolitov na verižno reakcijo s polimerazo (PCR)

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