Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation

ABSTRACT Neisseria meningitidis is an inhabitant of the nasopharynx, from which it is transmitted from person to person or disseminates in blood and becomes a harmful pathogen. In this work, we addressed colonization of the nasopharyngeal niche by focusing on the interplay between meningococci and t...

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Autores principales:	Mathilde Audry, Catherine Robbe-Masselot, Jean-Philippe Barnier, Benoit Gachet, Bruno Saubaméa, Alain Schmitt, Sophia Schönherr-Hellec, Renaud Léonard, Xavier Nassif, Mathieu Coureuil
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	airway mucus host-pathogen interaction meningitis nasopharynx Neisseria meningitidis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/d81ef51b21d74508b970f4b356236801
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spelling	oai:doaj.org-article:d81ef51b21d74508b970f4b3562368012021-11-15T15:22:24ZAirway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation10.1128/mSphere.00494-192379-5042https://doaj.org/article/d81ef51b21d74508b970f4b3562368012019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00494-19https://doaj.org/toc/2379-5042ABSTRACT Neisseria meningitidis is an inhabitant of the nasopharynx, from which it is transmitted from person to person or disseminates in blood and becomes a harmful pathogen. In this work, we addressed colonization of the nasopharyngeal niche by focusing on the interplay between meningococci and the airway mucus that lines the mucosa of the host. Using Calu-3 cells grown in air interface culture (cells grown with the apical domain facing air), we studied meningococcal colonization of the mucus and the host response. Our results suggested that N. meningitidis behaved like commensal bacteria in mucus, without interacting with human cells or actively transmigrating through the cell layer. As a result, type IV pili do not play a role in this model, and meningococci did not trigger a strong innate immune response from the Calu-3 cells. Finally, we have shown that this model is suitable for studying interaction of N. meningitidis with other bacteria living in the nasopharynx and that Streptococcus mitis, but not Moraxella catarrhalis, can promote meningococcal growth in this model. IMPORTANCE N. meningitidis is transmitted from person to person by aerosol droplets produced by breathing, talking, or coughing or by direct contact with a contaminated fluid. The natural reservoir of N. meningitidis is the human nasopharynx mucosa, located at the back of the nose and above the oropharynx. The means by which meningococci cross the nasopharyngeal wall is still under debate, due to the lack of a convenient and relevant model mimicking the nasopharyngeal niche. Here, we took advantage of Calu-3 cells grown in air interface culture to study how meningococci colonize the nasopharyngeal niche. We report that the airway mucus is both a niche for meningococcal growth and a protective barrier against N. meningitidis infection. As such, N. meningitidis behaves like commensal bacteria and is unlikely to induce infection without an external trigger.Mathilde AudryCatherine Robbe-MasselotJean-Philippe BarnierBenoit GachetBruno SaubaméaAlain SchmittSophia Schönherr-HellecRenaud LéonardXavier NassifMathieu CoureuilAmerican Society for Microbiologyarticleairway mucushost-pathogen interactionmeningitisnasopharynxNeisseria meningitidisMicrobiologyQR1-502ENmSphere, Vol 4, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	airway mucus host-pathogen interaction meningitis nasopharynx Neisseria meningitidis Microbiology QR1-502
spellingShingle	airway mucus host-pathogen interaction meningitis nasopharynx Neisseria meningitidis Microbiology QR1-502 Mathilde Audry Catherine Robbe-Masselot Jean-Philippe Barnier Benoit Gachet Bruno Saubaméa Alain Schmitt Sophia Schönherr-Hellec Renaud Léonard Xavier Nassif Mathieu Coureuil Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
description	ABSTRACT Neisseria meningitidis is an inhabitant of the nasopharynx, from which it is transmitted from person to person or disseminates in blood and becomes a harmful pathogen. In this work, we addressed colonization of the nasopharyngeal niche by focusing on the interplay between meningococci and the airway mucus that lines the mucosa of the host. Using Calu-3 cells grown in air interface culture (cells grown with the apical domain facing air), we studied meningococcal colonization of the mucus and the host response. Our results suggested that N. meningitidis behaved like commensal bacteria in mucus, without interacting with human cells or actively transmigrating through the cell layer. As a result, type IV pili do not play a role in this model, and meningococci did not trigger a strong innate immune response from the Calu-3 cells. Finally, we have shown that this model is suitable for studying interaction of N. meningitidis with other bacteria living in the nasopharynx and that Streptococcus mitis, but not Moraxella catarrhalis, can promote meningococcal growth in this model. IMPORTANCE N. meningitidis is transmitted from person to person by aerosol droplets produced by breathing, talking, or coughing or by direct contact with a contaminated fluid. The natural reservoir of N. meningitidis is the human nasopharynx mucosa, located at the back of the nose and above the oropharynx. The means by which meningococci cross the nasopharyngeal wall is still under debate, due to the lack of a convenient and relevant model mimicking the nasopharyngeal niche. Here, we took advantage of Calu-3 cells grown in air interface culture to study how meningococci colonize the nasopharyngeal niche. We report that the airway mucus is both a niche for meningococcal growth and a protective barrier against N. meningitidis infection. As such, N. meningitidis behaves like commensal bacteria and is unlikely to induce infection without an external trigger.
format	article
author	Mathilde Audry Catherine Robbe-Masselot Jean-Philippe Barnier Benoit Gachet Bruno Saubaméa Alain Schmitt Sophia Schönherr-Hellec Renaud Léonard Xavier Nassif Mathieu Coureuil
author_facet	Mathilde Audry Catherine Robbe-Masselot Jean-Philippe Barnier Benoit Gachet Bruno Saubaméa Alain Schmitt Sophia Schönherr-Hellec Renaud Léonard Xavier Nassif Mathieu Coureuil
author_sort	Mathilde Audry
title	Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
title_short	Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
title_full	Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
title_fullStr	Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
title_full_unstemmed	Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation
title_sort	airway mucus restricts <named-content content-type="genus-species">neisseria meningitidis</named-content> away from nasopharyngeal epithelial cells and protects the mucosa from inflammation
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/d81ef51b21d74508b970f4b356236801
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Airway Mucus Restricts <named-content content-type="genus-species">Neisseria meningitidis</named-content> Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation

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