Substrate properties modulate cell membrane roughness by way of actin filaments

Abstract Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions. In order to know whether membrane roughness is associated with the substrate properties, we employed the non-interferometric wide-field optical profilometry (NIWOP) technique to me...

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Autores principales:	Chao-Hung Chang, Hsiao-Hui Lee, Chau-Hwang Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d82a20de1c914b4984e6bfb6d1f1256a
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spelling	oai:doaj.org-article:d82a20de1c914b4984e6bfb6d1f1256a2021-12-02T12:32:17ZSubstrate properties modulate cell membrane roughness by way of actin filaments10.1038/s41598-017-09618-y2045-2322https://doaj.org/article/d82a20de1c914b4984e6bfb6d1f1256a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09618-yhttps://doaj.org/toc/2045-2322Abstract Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions. In order to know whether membrane roughness is associated with the substrate properties, we employed the non-interferometric wide-field optical profilometry (NIWOP) technique to measure the membrane roughness of living mouse embryonic fibroblasts with different conditions of the culture substrate. By controlling the surface density of fibronectin (FN) coated on the substrate, we found that cells exhibited higher membrane roughness as the FN density increased in company with larger focal adhesion (FA) sizes. The examination of membrane roughness was also confirmed with atomic force microscopy. Using reagents altering actin or microtubule cytoskeletons, we provided evidence that the dynamics of actin filaments rather than that of microtubules plays a crucial role for the regulation of membrane roughness. By changing the substrate rigidity, we further demonstrated that the cells seeded on compliant gels exhibited significantly lower membrane roughness and smaller FAs than the cells on rigid substrate. Taken together, our data suggest that the magnitude of membrane roughness is modulated by way of actin dynamics in cells responding to substrate properties.Chao-Hung ChangHsiao-Hui LeeChau-Hwang LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chao-Hung Chang Hsiao-Hui Lee Chau-Hwang Lee Substrate properties modulate cell membrane roughness by way of actin filaments
description	Abstract Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions. In order to know whether membrane roughness is associated with the substrate properties, we employed the non-interferometric wide-field optical profilometry (NIWOP) technique to measure the membrane roughness of living mouse embryonic fibroblasts with different conditions of the culture substrate. By controlling the surface density of fibronectin (FN) coated on the substrate, we found that cells exhibited higher membrane roughness as the FN density increased in company with larger focal adhesion (FA) sizes. The examination of membrane roughness was also confirmed with atomic force microscopy. Using reagents altering actin or microtubule cytoskeletons, we provided evidence that the dynamics of actin filaments rather than that of microtubules plays a crucial role for the regulation of membrane roughness. By changing the substrate rigidity, we further demonstrated that the cells seeded on compliant gels exhibited significantly lower membrane roughness and smaller FAs than the cells on rigid substrate. Taken together, our data suggest that the magnitude of membrane roughness is modulated by way of actin dynamics in cells responding to substrate properties.
format	article
author	Chao-Hung Chang Hsiao-Hui Lee Chau-Hwang Lee
author_facet	Chao-Hung Chang Hsiao-Hui Lee Chau-Hwang Lee
author_sort	Chao-Hung Chang
title	Substrate properties modulate cell membrane roughness by way of actin filaments
title_short	Substrate properties modulate cell membrane roughness by way of actin filaments
title_full	Substrate properties modulate cell membrane roughness by way of actin filaments
title_fullStr	Substrate properties modulate cell membrane roughness by way of actin filaments
title_full_unstemmed	Substrate properties modulate cell membrane roughness by way of actin filaments
title_sort	substrate properties modulate cell membrane roughness by way of actin filaments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d82a20de1c914b4984e6bfb6d1f1256a
work_keys_str_mv	AT chaohungchang substratepropertiesmodulatecellmembraneroughnessbywayofactinfilaments AT hsiaohuilee substratepropertiesmodulatecellmembraneroughnessbywayofactinfilaments AT chauhwanglee substratepropertiesmodulatecellmembraneroughnessbywayofactinfilaments
_version_	1718394125181517824

Substrate properties modulate cell membrane roughness by way of actin filaments

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