Potential Bacterial Antagonists for the Control of Charcoal Rot (Macrophomina phaseolina) in Strawberry

The effect of antagonistic bacteria to control Macrophomina phaseolina was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from Athrocaulon macrostachyum rhizosphere of the Lebrija marsh, were screened...

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Autores principales:	Josefina Viejobueno, Natalia Rodríguez-Berbel, Luis Miranda, Berta de los Santos, María Camacho
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biocontrol <i>Macrophomina phaseolina</i> <i>Brevibacterium</i> strawberry PGPR antagonistic bacteria Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/d82ec549066a4863bbbcbe7c9cf50bb5
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spelling	oai:doaj.org-article:d82ec549066a4863bbbcbe7c9cf50bb52021-11-25T17:47:20ZPotential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry10.3390/horticulturae71104572311-7524https://doaj.org/article/d82ec549066a4863bbbcbe7c9cf50bb52021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/457https://doaj.org/toc/2311-7524The effect of antagonistic bacteria to control <i>Macrophomina phaseolina</i> was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from <i>Athrocaulon macrostachyum</i> rhizosphere of the Lebrija marsh, were screened for their potential against <i>M. phaseolina</i> (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against <i>M. phaseolina</i> in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to <i>Brevibacterium</i> genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to <i>M. phaseolina</i> decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that <i>Brevibacterium</i> sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.Josefina ViejobuenoNatalia Rodríguez-BerbelLuis MirandaBerta de los SantosMaría CamachoMDPI AGarticlebiocontrol<i>Macrophomina phaseolina</i><i>Brevibacterium</i>strawberryPGPRantagonistic bacteriaPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 457, p 457 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biocontrol <i>Macrophomina phaseolina</i> <i>Brevibacterium</i> strawberry PGPR antagonistic bacteria Plant culture SB1-1110
spellingShingle	biocontrol <i>Macrophomina phaseolina</i> <i>Brevibacterium</i> strawberry PGPR antagonistic bacteria Plant culture SB1-1110 Josefina Viejobueno Natalia Rodríguez-Berbel Luis Miranda Berta de los Santos María Camacho Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
description	The effect of antagonistic bacteria to control <i>Macrophomina phaseolina</i> was evaluated under in vitro, growth chamber, greenhouse and field conditions. A total of 177 bacteria, isolated from <i>Athrocaulon macrostachyum</i> rhizosphere of the Lebrija marsh, were screened for their potential against <i>M. phaseolina</i> (causes charcoal rot in strawberry) by dual culture assay. Of these isolates, 14 most promising strains were molecularly identified by the 16S rDNA sequencing method using the EzBioCloud database. These strains were tested for in vitro hydrolytic enzymes, HCN production, and biocontrol against <i>M. phaseolina</i> in strawberry plants. All the 14 strains produced, at least, one hydrolytic enzymatic activity and one of them, which belongs to <i>Brevibacterium</i> genus (Hvs8), showed the lowest records of disease incidence (20%) and severity (0.4). With these results, greenhouse and field trials were carried out with the Hvs8 strain, compared to non-treated control. In the greenhouse assays, Hvs8 strain increased root dry mass by 30%, over the control. In the field trials, production and fruit quality were not significantly different between Hvs8 treatment and non-treated control, but plant mortality and plant mortality associated to <i>M. phaseolina</i> decreased by more than 24% and 65% respectively, in Hvs8 treatment. This study suggests that <i>Brevibacterium</i> sp. Hvs8 strain could be a candidate for controlling charcoal rot in strawberry.
format	article
author	Josefina Viejobueno Natalia Rodríguez-Berbel Luis Miranda Berta de los Santos María Camacho
author_facet	Josefina Viejobueno Natalia Rodríguez-Berbel Luis Miranda Berta de los Santos María Camacho
author_sort	Josefina Viejobueno
title	Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
title_short	Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
title_full	Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
title_fullStr	Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
title_full_unstemmed	Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry
title_sort	potential bacterial antagonists for the control of charcoal rot (<i>macrophomina phaseolina</i>) in strawberry
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d82ec549066a4863bbbcbe7c9cf50bb5
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Potential Bacterial Antagonists for the Control of Charcoal Rot (<i>Macrophomina phaseolina</i>) in Strawberry

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